Potential use of lymphocyte blastogenic responses in diagnosis of bovine tuberculosis☆

Thoen, Charles O.; Jarnagin, Jerald L.; Muscoplat, Charles C; Cram, L.S.; Johnson, Donald W.; Harrington, R

doi:10.1016/0147-9571(80)90012-0

Cited by 17 publications

(9 citation statements)

References 3 publications

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“…Cattle, humans and some other anitnal species preferentially mount a strong cellular response to mycobacterial infections [9]. In vitro cellular tests such as lymphocyte proliferation have been shown to be of potential use in diagnosing tuberculosis in cattle [10,11] although they are expensive and labour intensive to perform. Recently, the interferon-y (IFN-y) assay has been described as an in vitro diagnostic test for tuberculosis in cattle [12.…”

Section: Introductionmentioning

confidence: 99%

Cellular and Humoral Immune Responses of Cattle to Purified Mycobacterium bovis Antigens

et al. 1994

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Cellular responses to several purified antigens of Mycobacterium bovis were examined in experimentally infected cattle over a period of 36 months, using in vitro cellular proliferation and interferon-gamma assays. These antigens (12, 19, 22a, b, 24, 25, 30, 32, 39, 65 and 70 kDa) included the majority of M. bovis protein antigens described to date and are highly homologous to those purified from M. tuberculosis. Cellular responses were examined at 3-month time intervals during the 36-month course of infection. All purified antigens induced cellular immune responses in the infected animals. The onset and magnitude of response to individual antigens varied among the animals. At any specific time during the period of infection one or more antigens appeared to be immunodominant but the immunodominance profile changed as the infection progressed. Humoral immune responses were low or absent in the first half of the infection period, but increased substantially for some of the antigens during the second half. Variation was observed among the different animals as to which antigens they recognized.

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Section: Introductionmentioning

confidence: 99%

Cellular and Humoral Immune Responses of Cattle to Purified Mycobacterium bovis Antigens

et al. 1994

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“…In vitro lymphocyte proliferation under PPD stimulation after in vivo sensitization has been generally considered a good parameter of cell immune reactivity also with regard to cattle. It has been previously used to study or to identify tuberculosis infection (T hoen et al 1980; M uscoplat et al 1979; R ossi et al 1978; W oodard et al 1979; B ergman 1980). Phagocytosis has been demonstrated as central activity in immune response to tuberculosis (L owrie et al 1985).…”

Section: Introductionmentioning

confidence: 99%

BoLA class I polymorphism and in vitro immune response to M. bovis antigens

Longeri

Polli

Ponti

et al. 1993

J Animal Breeding Genetics

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From a sample of 119 Friesian calves, serologically typed for BoLA class I, 47 subjects were chosen expressing 9 different MHC types (A6, A6.9, A10, A11, A14, A15, A30, W16, M103) with the same age and reared in the same farm conditions. The animals were s.c. injected with a water in oil suspension of killed M. bovis and the treatment was repeated two days later. Before the treatment and 21 days later, calves were bled and on PBM (peripheral blood mononuclear leucocytes) were performed the following tests: 1. Lymphocyte Stimulation with bovine and avian PPDs (Purified protein derivative of Mycobacterium bovis and Mycobacterium avium, respectively). 2. Phagocytic activity towards M. bovis. 3. Class II molecules expression on cell surface. 4. Percentage of leucocyte populations and subpopulations. In the in vitro Lymphocyte Stimulation test, all the animals and classes were responders. Animals bearing A10 BoLA class I presented c.p.m. (counts per minute) and index values higher than the other cattle; these values were significantly positively related both to bovine and avian PPDs (P < .01). By variance analysis A14 BoLA type showed a slight positive significant correlation with more efficient phagocytic activity. BoLA class I type did not seem to significantly affect percentage of class II positive cells and leucocyte percentages on PBM. ZUSAMMENFASSUNG: Der BoLA Klasse I Polymorphismus und in vitro immunologische Antwort gegen die Antigene von M. bovis Aus einer Stichprobe von 119 für BoLA Klasse I serologisch typisierten Friesian Kälber, wurden 47 Subjekte ausgewählt, die 9 verschiedene MHC Typen ausdrückten (A6, A6.9, A10, A11, A14, A15, A30, W16, M103). Alle waren gleich alt und in gleichen Haltungsbedingungen. Die Tiere wurden mit einer Wasser-in-Ol Suspension abgetöteter M. bovis subkutan injiziert und die Behandlung nach zwei Tagen wiederholt. Vor und 21 Tage nach Behandlung wurden die folgenden Tests ausgeführt: 1. Lymphozyten-Stimulationstest mit bovinen und Geflügel PPDs. 2. Phagozyten Aktivität gegen M. bovis. 3. Zeil-Oberflächen, Expression der Klasse II Moleküle. 4. Anteile der Lymphozyten Populationen und Subpopulationen. Im in vitro Lymphozyten-Stimulationstest waren alle Tiere und Klassen responder. Tiere mit A10 BoLA I zeigten höhere c.p.m. und Indexwerte als die anderen; diese Werte waren in signifikant positiver Beziehung mit der PPD von M. bovis und auch mit M. avium (P < .01). BoLA Typ A14 zeigte leicht signifikant positive Korrelation mit wirksamerer Phagozyten Aktivität. BoLA Klasse I Typ scheint nicht den Prozentsatz der positiven Zellen der Klasse II und der Leukozyten der PBM signifikant zu beeinflussen. RESUMEN: Polimorfismo de BoLA clase I y immunidad a los antigenos del M. bovis Se escojeron 47 novillos dentro de un grupo de 119 animales que segun analisis previamente hecha tenian BoLA de clase I. Estos 47 novillos fueron escojidos de manera que tuvieran 9 distintos tipos de MHC (A6, A6.9, A10, A11, A14, A15, A30, W16, M103), la misma edad, las mismas condiciones de cria. Estos animales...

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“…Since M. bovis is an intracellular pathogen, diagnostic assays based on the conserved cell-mediated immune (CMI) response have shown greater promise for detecting a spectrum of M. bovis-infected animals. The lymphocyte proliferation assay (LPA) was the first-reported in vitro correlate of the in vivo delayed-type hypersensitivity response from M. bovis-infected animals (20) and was shown to be more sensitive than serological assays for a variety of species (31). Such proliferation assays, however, are generally re-garded as unsuitable outside research applications because of requirements for expensive equipment, long incubation periods in complex tissue culture media, and the use of radioactive nucleotides and because they may also measure B-lymphocyte proliferation.…”

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confidence: 99%

Development and Evaluation of a Real-Time Reverse Transcription-PCR Assay for Quantification of Gamma Interferon mRNA To Diagnose Tuberculosis in Multiple Animal Species

Harrington

Surujballi

Waters

et al. 2007

Clin Vaccine Immunol

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Tuberculosis of free-ranging and captive wildlife, including species implicated in the maintenance and transmission of Mycobacterium bovis, is a difficult disease to diagnose and control. Historically, diagnosis of tuberculosis has relied largely upon assays of cell-mediated immunity (CMI), such as tuberculin skin testing. This approach, however, is problematic or impractical for use with many wildlife species. Increasingly, in vitro diagnostic tests, including gamma interferon (IFN-␥)-based assays, are replacing or complementing skin testing of cattle and humans. Analogous assays are unavailable for most wildlife because of a lack of species-specific immunological reagents. This report describes the development and validation of a wholeblood assay to quantify antigen-specific IFN-␥ mRNA expression by quantitative real-time reverse transcription-PCR. Oligonucleotide primers and probes were designed and tested for reactivity towards several susceptible species of interest with respect to tuberculosis infection. The assay was subsequently optimized to quantify the IFN-␥ mRNA expression in elk and red deer (Cervus elaphus) and was evaluated for its ability to detect mycobacterial antigen-specific responses of experimentally tuberculosis-infected animals. The assay was a simple, rapid, and sensitive measure of antigen-specific CMI. The IFN-␥ mRNA responses correlated well with IFN-␥ protein production and showed performance in determining an animal's infection status superior to that of either lymphocyte proliferation or IFN-␥ protein enzyme-linked immunosorbent assay methods. An additional advantage is the ease with which the assay can be modified to reliably quantify IFN-␥ expression by using consensus sequences of closely related species or of other species for which IFN-␥ sequence information is available.Mycobacterium bovis, the etiological agent of bovine tuberculosis (TB), has an extraordinarily broad mammalian host range that includes humans, domestic livestock, and wildlife. Globally, several wildlife species have been implicated in the maintenance and transmission of M. bovis: the badger (Meles meles) in Great Britain and Ireland (1), the brushtail possum (Trichosurus vulpecula) in New Zealand (2), white-tailed deer (Odocoileus virginianus) in Michigan (28), elk (Cervus elaphus) in Manitoba, Canada (17), and buffalo (Syncerus caffer) and kudu (Tragelaphus strepsiceros) in southern Africa (15). These wildlife hosts are difficult to control, and the transmission of M. bovis to domestic livestock may result in regulatory action, movement restrictions, or isolation of infected individuals and may become a significant barrier to the success of national eradication and control programs. Transmission among freeranging and captive wildlife also creates problems in the management of zoological collections; the risk of infection to other valuable animals is increased, directly affecting the value of the collection, and in some cases, the value of tourism or related economic benefits to a country (15).New control str...

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Potential use of lymphocyte blastogenic responses in diagnosis of bovine tuberculosis☆

Cited by 17 publications

References 3 publications

Cellular and Humoral Immune Responses of Cattle to Purified Mycobacterium bovis Antigens

Cellular and Humoral Immune Responses of Cattle to Purified Mycobacterium bovis Antigens

BoLA class I polymorphism and in vitro immune response to M. bovis antigens

Development and Evaluation of a Real-Time Reverse Transcription-PCR Assay for Quantification of Gamma Interferon mRNA To Diagnose Tuberculosis in Multiple Animal Species

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