Potential use of glycerol as substrate for the production of red pigments by Monascus ruber in submerged fermentation

Meinicke, Rose Marie; Vendruscolo, Francielo; Moritz, Denise Esteves; Oliveira, Débora de; Schmidell, Willibaldo; Samohyl, Robert Wayne; Ninow, Jorge Luiz

doi:10.1016/j.bcab.2012.03.001

Cited by 45 publications

(27 citation statements)

References 19 publications

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“…In previous studies (MEINICKE et al, 2012), maximum production of 7.38 UA of red pigments in flasks using glycerol as the only synthetic substrate was observed. Pastrana et al (1995) studied the production of pigments in a 20 L bioreactor using glucose as a substrate, and they obtained 9.7 UA of red pigment.…”

Section: Resultsmentioning

confidence: 81%

Monascus pigment production in bioreactor using a co-product of biodiesel as substrate

Bühler¹,

Dutra²,

Vendruscolo³

et al. 2013

Ciênc. Tecnol. Aliment.

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Section: Resultsmentioning

confidence: 81%

Monascus pigment production in bioreactor using a co-product of biodiesel as substrate

Bühler¹,

Dutra²,

Vendruscolo³

et al. 2013

Ciênc. Tecnol. Aliment.

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“…The plates were incubated at 28 ±2 o C for 7 days [25]. The red dye-producing colony was picked and purified on the same medium.…”

Section: Isolation and Identification Of The Pigments-producing Fungusmentioning

confidence: 99%

“…Glycerol and glutamate as substrate on the red pigments production by M. ruber affected the production, where it was 40-70 g L -1 and 7-8 g L -1 for glycerol and glutamate, respectively [25]. Effect of different amino acids on bio-pigments production showed that histidine was the most valuable amino acid resulted in the highest production of red pigments and almost completely eliminated the formation of the mycotoxin [26].…”

Section: Introductionmentioning

confidence: 98%

Effect of media composition on citrinin and bio-pigments production by Monascus ruber

2017

J App Biol Biotech

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Monascus species are used for production of red bio-pigments, which are used as food coloring agents. However, presence of the mycotoxin citrinin, which is a secondary metabolite produced in conjunction with the red pigments, prevents the use of these pigments in large scale. In the present study, Monascus ruber Van Tieghem was isolated from the Egyptian soil and identified. Effect of media composition on nine of the pigments produced by this fungus in addition to citrinin was studied. The fungus was grown in four different media containing different carbon sources (glucose, ethanol, yeast malt extract and potato dextrose broth). On the other hand, effect of glucose concentration was also studied. The produced pigments and citrinin were extracted by butanol and analyzed by LC/MS/MS as well as HPLC with fluorescence detection of citrinin. The data revealed that the highest amount of red pigments production (4.3g/l) was obtained at glucose concentration 10g/l. This was combined with complete absence of citrinin. Rubropunctamine (26.1%) and ankuflavine (48.7%) were the most abundant pigments in the glucose and ethanol media, respectively; while Moanascin (61.6%) and N-glutarylrubropunctamine (39.4%) were the most abundant in the PDB and YMB media, respectively. This result enables the large scale production of the red pigments by M. ruber without production of the mycotoxin citrinin.

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“…For comparative issues, the amounts of biomass and red pigment (monascorubramine; C 23 H 27 NO 4 ), at the end of fermentation, were measured according to the methods employed by Meinicke et al 13 The values obtained for the last fermentation for these parameters were of 4.03 ± 0.21 g L −1 and 3.96 ± 0.17 UA 480 (optical density units; 480 nm), respectively. These are satisfactory values for fermentation, considering the type of culture medium low enriched as the rice powder.…”

Section: Performance Of the Fermentationsmentioning

confidence: 99%

“…13 Three fermentations (Ferm.01, 02 and 03) were performed within periods of five, eight and ten days, respectively, all with the same fermenter and in the same conditions. The fermenter was made of glass (Dist, Florianópolis, Brazil), with a coupling plug at the top for fitting hoses and an air outlet directed to a collector cartridge with "XAD-2".…”

mentioning

confidence: 99%

Study of Viability of Solid-Phase Microextraction, in vivo, in the Extraction of Microbial Volatile Organic Compounds Associated to the Pigment Production Process by the Monascus Fungus, in Submerged Fermentation

Schacker

Moritz

Miguel

et al. 2016

J. Braz. Chem. Soc.

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Microbial volatile organic compounds (MVOCs) are present in several production processes in biotechnology. In this study it is evaluated the viability of use of a method involving solidphase microextraction (SPME), in vivo SPME, in the extraction of MVOCs, associated with the pigment production process by the Monascus fungus. PDMS/Car/DVB (50/30 μm) fiber in the headspace mode (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) were used to fermentation broth study. Seventeen compounds were found associated with the presence of the fungus, being the main ones ethanol, 2-methyl-propanol, 3-methyl-butanol, 2-methyl-butanol and 2-phenyl-ethanol. These compounds have maximum concentration of 313.87, 5.51, 2.37, 0.95 and 0.70 mg L −1 . The values of biomass and red pigment found for fermentation for ten days were 4.03 ± 0.21 g L −1 and 3.96 ± 0.17 (UA 480 ). Regular MVOC productions were observed in this study, which were useful in the prediction and control of fermentation by Monascus fungus.

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Potential use of glycerol as substrate for the production of red pigments by Monascus ruber in submerged fermentation

Cited by 45 publications

References 19 publications

Monascus pigment production in bioreactor using a co-product of biodiesel as substrate

Monascus pigment production in bioreactor using a co-product of biodiesel as substrate

Effect of media composition on citrinin and bio-pigments production by Monascus ruber

Study of Viability of Solid-Phase Microextraction, in vivo, in the Extraction of Microbial Volatile Organic Compounds Associated to the Pigment Production Process by the Monascus Fungus, in Submerged Fermentation

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