Potential use of embalmed cadavers to study mast cell presence

MacBride, Robert G.

doi:10.1002/(sici)1097-0185(199801)250:1<117::aid-ar12>3.0.co;2-r

Cited by 4 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Embalmed cadaveric material has generally been avoided for histological purposes, as the fixation achieved through the process of whole body embalming has been considered inadequate for histological specimens (MacBride 1998). The quality of embalmed tissue is affected by the time that elapses between death and the embalming process, the patency of the peripheral blood vessels, and the age and cause of death of the individual.…”

Section: Introductionmentioning

confidence: 99%

A comparison of different embalming fluids on the quality of histological preservation in human cadavers

Nicholson¹,

Samalia²,

Gould³

et al. 2006

European Journal of Morphology

View full text Add to dashboard Cite

There are significant problems in obtaining normal human material for histology for teaching or research purposes. This study shows that tissue from cadavers embalmed for teaching can be used for routine histology. Twelve cadavers embalmed with four different formalin-containing embalming fluids were used (n = 3 per fluid): (1) formalin mix (10% formalin); (2) Dunedin mix (an alcohol-based fluid containing phenol); (3) Michigan mix (a water-based fluid); and (4) phenoxyethanol mix (an alcohol-based fluid containing phenoxyethanol). Tissue blocks of liver, heart, kidney, skin and skeletal muscle were taken from each cadaver, paraffin embedded, sectioned and stained with haematoxylin and eosin (H & E), Periodic Acid Schiff (PAS), or Mallory trichrome (Malt). Each section was assigned an overall score based on the histological quality of the cellular components of the tissue. Sections were scored from 1 to 3 (1 = poor, 2 = satisfactory, 3 = good). Satisfactory sections were obtained from all cadavers except those embalmed with the Dunedin mix. The Michigan and phenoxyethanol fluids resulted in consistently good quality sections. No significant differences in tissue morphology were observed between the different stains. The clearest morphology was observed in the skin and skeletal muscle sections, and in tissues embalmed with fluids which do not contain phenol.

show abstract

Section: Introductionmentioning

confidence: 99%

A comparison of different embalming fluids on the quality of histological preservation in human cadavers

Nicholson¹,

Samalia²,

Gould³

et al. 2006

European Journal of Morphology

View full text Add to dashboard Cite

show abstract

“…Firstly, the study was carried out on muscle tissue from cadavers treated with a Formaldehyde based embalming fluid as per Glasgow University embalming protocols. This may result in the tissues becoming hard, rigid and often difficult to dissect [ 26 ]. MacBride [ 27 ] also suggested that studies using embalmed cadavers were often avoided because the fixation process was poor, making tissues less suitable for histological examination.…”

Section: Discussionmentioning

confidence: 99%

Preliminary observations of muscle fibre cross sectional area of flexor digitorum brevis in cadaver feet with and without claw toes

Locke

Baird

Frankis

2010

Journal of Foot and Ankle Research

View full text Add to dashboard Cite

BackgroundIn order to facilitate normal gait, toes require to be in a rectus position during the propulsive phase. This requires a correct balance and sequence of activity of the intrinsic musculature of the feet. Alteration of this balance and sequence may lead to the development of claw toes. Atrophy of the lumbricals occurs in the development of claw toes, but it is not known if changes occur in any other intrinsic muscles, including flexor digitorum brevis. This study set out to investigate whether hypertrophic changes were evident in flexor digitorum brevis in feet with claw toes.MethodsFour cadaver feet were investigated, two with rectus toes and two with claw toes. Flexor digitorum brevis was removed from each, and seven anatomically significant tissue sections from each muscle were routinely processed, cut and stained. One hundred and sixty muscle fibre cross sectional areas were measured from each section.ResultsThe mean age of the donors was 81.5 years, and three of the four were female. Results showed that the cross sectional area of fibres from feet with claw toes was 417 μg2 significantly greater (p < 0.01) than the cross sectional area of fibres from feet with rectus toes, which was 263 μg2.ConclusionsAlthough this study has several limitations, preliminary observations reveal that flexor digitorum brevis muscle fibre cross sectional area is significantly reduced in feet with claw toes. This would indicate a relationship between muscle fibre atrophy of flexor digitorum brevis and clawing of the lesser toes.

show abstract

“…Electron-microscopic studies have demonstrated that avidin binds highly preferentially to individual mast cell granules rather than to other cellular structures (Bussolati and Gugliotta 1983; Jones et al 1987; Tharp et al 1985), and rodent or human mast cells are known to be readily stained with avidin conjugated to horseradish peroxidase (Bussolati and Gugliotta 1983; Kasper and Tharp 1987) or to certain fluorochrome dyes (Tharp et al 1985; Markey et al 1989; MacBride 1998). Based on the results, we decided to use an adivin gold (AvG) complex as a cationic probe for detection of anionic sites in mast cell granules and other structures.…”

Section: Introductionmentioning

confidence: 99%

Localization of anionic constituents in mast cell granules of brachymorphic (bm/bm) mice by using avidin-conjugated colloidal gold

et al. 2010

View full text Add to dashboard Cite

We used the egg avidin gold complex as a polycationic probe for the localization of negatively charged sites in the secretory granules of mouse mast cells. We compared the binding of this reagent to mast cell granules in wild-type mice and in congenic brachymorphic mice in which mast cell secretory granules contained undersulfated proteoglycans. We localized anionic sites by post-embedding labeling of thin sections of mouse skin and tongue tissues fixed in Karnovsky's fixative and OsO4 and embedded in Araldite. Transmission electron microscopy revealed that the mast cell granules of bm/bm mice had a lower optical density than those of wild-type mice (P<0.001) and a lower adivin gold binding density (by approximately 50%, P<0.001). The latter result provides additional evidence that the contents of mast cell granules in bm/bm mice were less highly sulfated than in those of wild type mice. In both wild type and bm/bm mast cells, the distribution of granule equivalent volumes was multimodal, but the unit granule volume was approximately 19% lower in bm/bm cells than in wild type cells (P < 0.05). Thus, bm/bm mast cells develop secretory granules that differ from those of wild type mice in exhibiting a lower optical density and slightly smaller unit granules, however the processes that contribute to granule maturation and granule-granule fusion in mast cells are operative in the bm/bm cells.

show abstract

Potential use of embalmed cadavers to study mast cell presence

Cited by 4 publications

References 7 publications

A comparison of different embalming fluids on the quality of histological preservation in human cadavers

A comparison of different embalming fluids on the quality of histological preservation in human cadavers

Preliminary observations of muscle fibre cross sectional area of flexor digitorum brevis in cadaver feet with and without claw toes

Localization of anionic constituents in mast cell granules of brachymorphic (bm/bm) mice by using avidin-conjugated colloidal gold

Contact Info

Product

Resources

About