Potential use of chitosan nanoparticles for oral delivery of DNA vaccine in Asian sea bass (Lates calcarifer) to protect from Vibrio (Listonella) anguillarum

Kumar, Saurav; Ahmed, V.P. Ishaq; Parameswaran, V.; Sudhakaran, R.; Babu, V. Sarath; Hameed, A.S. Sahul

doi:10.1016/j.fsi.2007.12.004

Cited by 179 publications

(44 citation statements)

References 43 publications

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“…The health status of the fish used in this study was determined by collecting a representative random sample of 12 fish that were used for bacteriology, pathology, and histopathology examination. Bacterial pathogens examined were Aeromonas hydrophila and E. tarda , shown to infect different fish species in India [16,17,31] and the organs examined included the head kidney, spleen, pancreas, liver, gill, heart, and muscle. No bacterial infections, pathology, or histopathological changes were detected in any organs examined from the sampled fish.…”

Section: Methodsmentioning

confidence: 99%

Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)

et al. 2016

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The use of oral vaccination in finfish has lagged behind injectable vaccines for a long time as oral vaccines fall short of injection vaccines in conferring protective immunity. Biodegradable polymeric nanoparticles (NPs) have shown potential to serve as antigen delivery systems for oral vaccines. In this study the recombinant outer membrane protein A (rOmpA) of Edwardsiella tarda was encapsulated in chitosan NPs (NP-rOmpA) and used for oral vaccination of Labeo fimbriatus. The rOmpA purity was 85%, nanodiameter <500 nm, encapsulation efficiency 60.6%, zeta potential +19.05 mV, and there was an in vitro release of 49% of encapsulated antigen within 48 h post incubation in phosphate-buffered saline. Empty NPs and a non-formulated, inactivated whole cell E. tarda (IWC-ET) vaccine were used as controls. Post-vaccination antibody levels were significantly (p = 0.0458) higher in the NP-rOmpA vaccinated fish (Mean OD450 = 2.430) than in fish vaccinated with inactivated whole cell E. tarda (IWC-ET) vaccine (Mean OD450 = 1.735), which corresponded with post-challenge survival proportions (PCSP) of 73.3% and 48.28% for the NP-rOmpA and IWC-ET groups, respectively. Serum samples from NP-rOmpA-vaccinated fish had a higher inhibition rate for E. tarda growth on tryptic soy agar (TSA) than the IWC-ET group. There was no significant difference (p = 0.989) in PCSPs between fish vaccinated with empty NPs and the unvaccinated control fish, while serum from both groups showed no detectable antibodies against E. tarda. Overall, these data show that the NP-rOmpA vaccine produced higher antibody levels and had superior protection over the IWC-ET vaccine, showing that encapsulating OmpA in chitosan NPs confer improved protection against E. tarda mortality in L. fimbriatus. There is a need to elucidate the possible adjuvant effects of chitosan NPs and the immunological mechanisms of protective immunity induced by OMPs administered orally to fish.

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Section: Methodsmentioning

confidence: 99%

Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)

et al. 2016

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“…The microparticles protect the encapsulated DNA from degradation by DNase (Rajesh Kumar et al, 2008) and from shear forces . Notably, the DNA vaccine can transfect fi sh cells and present very low levels of cytotoxicity (Rajesh Kumar et al, 2008). Thanks to these characteristics, chitosans can be used to administer DNA and vaccines of diverse natures to fi sh orally or by injection.…”

Section: Chitosanmentioning

confidence: 99%

“…Three weeks after the vaccination, there was a low level of stimulation of antibodies of the fi sh. The protection against Vibrio anguillarum caused a 46% RPS (Rajesh Kumar et al, 2008). Also, when chitosan/ alginate microparticles were used to encapsulate Vibrio anguillarum or BSA (bovine serum albumine) it was found that more antigen is released with lower molecular weight of chitosan and that as with alginate, there is more antigen release in a basic (pH 9) rather than an acid environment (pH 3, Polk et al, 1994).…”

Section: Bacterin Antigens Encapsulated In Chitosanmentioning

confidence: 99%

Nanoparticles and microparticles of polymers and polysaccharides to administer fish vaccines

2013

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Aquaculture has become an important economic sector worldwide, but is faced with an ongoing threat from infectious diseases. Vaccination plays a critical role in protecting commercially raised fi sh from bacterial, viral and parasitic diseases. However, the production of eff ective vaccines is limited by the scarcity of knowledge about the immune system of fi sh. Improving vaccines implies using antigens, adjuvants and employing methods of administration that are more eff ective and less harmful to the fi sh. In this context, in recent year there have studies of methods of encapsulating antigens in matrices of diff erent types to apply in fi sh vaccines. This work reviews the new methods to improve fi sh vaccines by encapsulating them in polymers and polysaccharides.

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“…At present there are no direct competitors for commercial fish-vaccines other than universities (Dipangka & Dibyendu, 2012;Harikrishnan, Balasundaram, & Heo, 2012;Maiti et al, 2012;Rajesh Kumar et al, 2008) and research institutes (Behera & Swain, 2012;Swain et al, 2010) which provide experimental vaccines. The most likely competitors in India are substitute manufacturers such as Hindustan Ciba-Geigy Ltd, Glaxo Laboratories, etc., who are supplying chemotherapeutic agents for aquaculture enterprises (Pathak, Ghosh, & Palanisamy, 2000).…”

Section: The Competitive Landscape In the Fish Vaccines Segment In Indiamentioning

confidence: 99%

Strategic Macro-Environmental Factor Analysis for Entry into the Fish Vaccines Market in India

Pallapothu

Krause

2013

IJBM

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Globally, the aquaculture segment continues to be the fastest growing food-fish producing segment and for India, the second largest food-fish producing country, it was forecasted that the segment would grow at an average annual growth rate of approximately 7.0% to reach a total production target of 8-10 million tons by 2020. Currently no commercial fish vaccines are available in India; therefore, the macro-environmental factors with respect to the competitive, political, economic, socio-cultural, technological, and legal landscapes, which influence a potential entrant's strategic business decisions to set up a fish vaccines company, are evaluated. The results indicate that although the macro-environmental factors in India are improving there are still some bureaucratic hurdles to be overcome by an entrant to assure success.

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Potential use of chitosan nanoparticles for oral delivery of DNA vaccine in Asian sea bass (Lates calcarifer) to protect from Vibrio (Listonella) anguillarum

Cited by 179 publications

References 43 publications

Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)

Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)

Nanoparticles and microparticles of polymers and polysaccharides to administer fish vaccines

Strategic Macro-Environmental Factor Analysis for Entry into the Fish Vaccines Market in India

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