Potential Toxicity of Phthalic Acid Esters Plasticizer: Interaction of Dimethyl Phthalate with Trypsin in Vitro

Abstract

Dimethyl phthalate (DMP) is widely used as a plasticizer in industrial processes and has been reported to possess potential toxicity to the human body. In this study, the interaction between DMP and trypsin in vitro was investigated. The results of fluorescence, UV–vis, circular dichroism, and Fourier transform infrared spectra along with cyclic voltammetric measurements indicated that the remarkable fluorescence quenching and conformational changes of trypsin resulted from the formation of a DMP–trypsin complex, which was driven mainly by hydrophobic interactions. The molecular docking and trypsin activity assay showed that DMP primarily interacted with the catalytic triad of trypsin and led to the inhibition of trypsin activity. The dimensions of the individual trypsin molecules were found to become larger after binding with DMP by atomic force microscopy imaging. This study offers a comprehensive picture of DMP–trypsin interaction, which is expected to provide insights into the toxicological effect of DMP.

Comparative studies on the interaction of nitrofuran antibiotics with bovine serum albumin

“…The secondary structure compositions of BSA and its nitrofuran complexes were estimated by applying the curve-tted process to t the Gaussian line shapes to the amide I band of the FT-IR spectral data. 18 2.4. Circular dichroism (CD) and resonance light scattering (RLS) studies for the interaction The CD spectra of BSA (2.5 Â 10 À7 mol L À1 ) in the presence of nitrofurans were recorded in the range of 200-250 nm under constant nitrogen ush.…”

Antihyperuricemic effect of dietary polyphenol sinapic acid commonly present in various edible food plants

“…The XOD solution was uniformly distributed via various intermolecular TA B L E 1 Effect of sinapic acid on body, liver, and kidney weight of PO-induced hyperuricemic mice (each value represents mean ± STD, n = 8) forces. Following the addition of SA into the XOD solution, there was no longer a uniform distribution and a new XOD structure was formed, as shown by the various sizes of circles ( Figure 4B,b) (Wang, Zhang, & Wang, 2014).…”

Positive Association between Urinary Concentration of Phthalate Metabolites and Oxidation of DNA and Lipid in Adolescents and Young Adults

“…However, DMP is one of the most frequently used phthalates. The impact of this chemical on the environment and its toxicity to living organisms is of great concern43. In animals and humans, increasing evidence suggests that lower molecular weight phthalates, such as DMP and its metabolites, may interfere with the development and reproductive systems4445.…”