Potential Toxicity of Differential Functionalized Multiwalled Carbon Nanotubes (MWCNT) in Human Cell Line (BEAS2B) and<i>Caenorhabditis elegans</i>

Chatterjee, Nivedita; Yang, Jaesung; Kim, Hyun-Mi; Jo, Eunhye; Kim, Philje; Choi, Kyunghee; Choi, Jinhee

doi:10.1080/15287394.2014.951756

Cited by 70 publications

(33 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While several studies indicated that COOH functionalization significantly decreased CNT bioactivities, e.g. fibrogenic responses and pulmonary inflammation49505152, we and some others observed an increase in cytogenic, genotoxic and fibrogenic activities by COOH functionalization5354. In the present study, f-MWCNT elicited more robust effects on CAF-like cells and CSCs as compared to MWCNT.…”

Section: Discussioncontrasting

confidence: 52%

Induction of cancer-associated fibroblast-like cells by carbon nanotubes dictates its tumorigenicity

Luanpitpong

Wang

Castranova

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Tumor microenvironment has been recognized as a key determinant of tumor formation and metastasis, but how tumor microenvironment is affected by nanomaterials is essentially unknown. Here, we investigated whether carbon nanotubes (CNTs), a widely used nanomaterial with known carcinogenic potential, can affect cancer-associated fibroblasts (CAFs), which are a key component of tumor microenvironment that provides necessary support for tumor growth. We show for the first time that single-walled CNT and to a lesser extent multi-walled and its COOH-functionalized form induced CAF-like cells, which are non-tumorigenic in animals, but promote tumor growth of human lung carcinoma and CNT-transformed lung epithelial cells. The mechanism by which CNT-induced CAF-like cells promote tumor growth involved the acquisition of cancer stem cells (CSCs) in cancer population. Gene knockdown experiments showed that an expression of podoplanin on CAF-like cells is essential for their effects, indicating the functional role of CAF-like cells and podoplanin in CNT tumorigenic process. Our findings unveil a novel mechanism of CNT-induced carcinogenesis through the induction of CAF-like cells that support CSCs and drive tumor formation. Our results also suggest the potential utility of podoplanin as a mechanism-based biomarker for rapid screening of carcinogenicity of CNTs and related nanomaterials for their safer design.

show abstract

Section: Discussioncontrasting

confidence: 52%

Induction of cancer-associated fibroblast-like cells by carbon nanotubes dictates its tumorigenicity

Luanpitpong

Wang

Castranova

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…As an example, nonfunctionalised nanotubes are shown to be more toxic to cells and animals compared to well‐functionalised dispersed CNTs (Liu, Tabakman, Welsher, & Dai, ). However contrasting results about biosafety of different CNT preparations have been reported (Allegri et al., ; Chatterjee et al., ; Hamilton, Wu, Mitra, Shaw, & Holian, ; Liu et al., ; Ursini et al., ).…”

Section: Discussionmentioning

confidence: 99%

In vitroreprotoxicity of carboxyl-functionalised single- and multi-walled carbon nanotubes on human spermatozoa

Aminzadeh

Jamalan²,

Chupani

et al. 2016

Andrologia

View full text Add to dashboard Cite

Reproductive toxicity of carboxyl-functionalised carbon nanotubes (CNT-COOH), as the most commonly used form of water-soluble CNTs, is not clearly studied. The aim of this study was to investigate in vitro toxicity of carboxylated single-walled and multi-walled CNTs (SWCNT-COOH and MWCNT-COOH) against human spermatozoa. Sperm cells from healthy donors were incubated with 0.1-100 μg/ml of SWCNT-COOH or MWCNT-COOH at 37°C for up to 5 hr. Viability of sperm cells was assessed using MTT test, and sperm motility was evaluated following World Health Organization guideline. Production of reactive oxygen species (ROS) and nitric oxide (NO) in sperm was also assessed. We showed that both MWCNT-COOH and SWCNT-COOH following incubation in vitro with human spermatozoa did not exert negative effect on viability while motility was significantly (p < .05) dropped in a dose-dependent manner. Moreover, there was no significant effect of the type, dose and exposure time of the CNT-COOH on NO production. Exposure of sperm cells to both examined types of CNTs at concentrations as low as 0.1 μg/ml caused a significant increase in ROS levels. In conclusion, carboxylated forms of CNTs seem to be harmful for human spermatozoa. Further studies, especially using in vivo models, are needed to decide about reprotoxicity of carboxylated forms of CNTs.

show abstract

“…Significantly, we find that the physical form of carbon has a major impact on toxicity. CNTs are more toxic than similar chemical amounts of carbon in amorphous carbon black, which is quite nontoxic even at the highest concentration (0.75 mg/kg) (Chatterjee et al, ). Thus, the molecular structure and topology is essential for the clastogenicity/genotoxicity of carbonaceous nanomaterials.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have reported the genotoxicity, cytotoxicity, pulmonary, and skin toxicity of SWCNTs (Smart et al, ; Naya et al, ; Reddy at al., ; Lindberg et al, ; Xu et al, ; Yu et al, ; Chatterjee et al, ; Farombi et al, ). Lindberg et al () in their genotoxicity study with human bronchial epithelial cells, observed an increase in DNA damage when exposed to a mixture of SWCNT and other carbon nanotubes.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic evaluation of functionalized single-walled carbon nanotube in mice bone marrow cells

et al. 2015

View full text Add to dashboard Cite

With their unique structure and physico-chemical properties, single walled carbon nanotubes (SWCNTs) have many potential new applications in medicine and industry. However, there is lack of detailed information concerning their impact on human health and the environment. The aim of this study was to assess the effects, after intra peritoneal injection of functionalized SWCNTs (f-SWCNT) on the induction of reactive oxygen species (ROS), frequency of structural chromosomal aberrations (SCA), frequency of micronuclei (MN) induction, mitotic index (MI) and DNA damage in Swiss-Webster mice. Three doses of f-SWCNTs (0.25, 0.5 and 0.75 mg/Kg) and two controls (negative and positive) were administered to mice, once a day for five days. Bone marrow and peripheral blood samples were collected 24 hours after the last treatment following standard protocols. F-SWCNT exposure significantly enhanced ROS, increased (p<0.05) the number of SCA and the frequency of micro-nucleated cells, increased DNA damage, and decreased the mitotic index in exposed groups compared to negative control. The scientific findings reported here suggest that purified f-SWCNT have the potential to induce oxidative stress mediated genotoxicity in Swiss-Webster mice at higher level of exposure. Further characterization of their systemic toxicity, genotoxicity, and carcinogenicity is also essential.

show abstract

Potential Toxicity of Differential Functionalized Multiwalled Carbon Nanotubes (MWCNT) in Human Cell Line (BEAS2B) andCaenorhabditis elegans

Cited by 70 publications

References 34 publications

Induction of cancer-associated fibroblast-like cells by carbon nanotubes dictates its tumorigenicity

Induction of cancer-associated fibroblast-like cells by carbon nanotubes dictates its tumorigenicity

In vitroreprotoxicity of carboxyl-functionalised single- and multi-walled carbon nanotubes on human spermatozoa

Cytogenetic evaluation of functionalized single-walled carbon nanotube in mice bone marrow cells

Contact Info

Product

Resources

About