Potential Therapeutic Effects of Melatonin Mediate via miRNAs in Cancer

Pourmohammad, Pirouz; Maroufi, Nazila Fathi; Rashidi, Mohsen; Vahedian, Vahid; Pouremamali, Farhad; Faridvand, Yousef; Ghaffari-Novin, Mahsa; Isazadeh, Alireza; Hajazimian, Saba; Nejabati, Hamid Reza; Nouri, Mohammad

doi:10.1007/s10528-021-10104-4

“…MiRNAs are possible novel therapeutic targets and biomarkers for cancer [16]. Studies have shown that miRNA regulates glioma cell genesis, development, proliferation, invasion, and metastasis [17]. MiR-339-5P expression is linked to lymphatic metastases and may inhibit mammary cancer cell proliferation, invasion, and metastatic potential [18].…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of miR-339-5p on Glioma through PTP4A1/HMGB1 Pathway

Zheng

¹

,

Wang

²

,

Shen

³

et al. 2022

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Objective. Finding miR-339-5p inhibitory functions in glioma through PTP4A1/HMGB1 pathway. Methods. From May 2020 to August 2021, 20 glioblastoma and para cancer tissues were chosen for qRT-PCR analysis. The miR-NC, miR-con, miR-339-5PMIMIC, and miR-con + groups were transfected into human glioma U251 cells. The capacity of cell vascular-like structure construction was found by simulating angiogenesis, and the ability of cell movement was examined by cell scratching. The twofold luciferase reporter gene method determined that miR-339-5p targets PTP4A1, and the protein expression levels of PTP4A1 and HMGB1 were examined using Western blot. Results. MiR-339-5P expression was substantially lower in cancer samples than noncancer samples ( P < 0.05 ). PTP4A1 expression in cancer samples was higher than in healthy controls ( P < 0.05 ). The miR-339-5p group produced significantly less vascular-like structures than the NC and miR-con groups ( P < 0.05 ). The miR-339-5p group lowered the invasive index and migratory rate of U251 cells ( P < 0.05 ). PTP4A1 inhibited the luciferase activity of the pTP4A1-WT reporter gene ( P < 0.05 ) but not the PTP4A1-MUT ( P > 0.05 ). The miR-339-5p group had lower protein levels of PTP4A1 and HMGB1 than the NC and miR-con groups ( P < 0.05 ). The development of vascular-like structures was substantially more significant in the miR-con +PTP4A1 group than in the miR-con and miR-339-5p +PTP4A1 groups ( P < 0.05 ). In terms of migration and invasion index, there was a substantial difference between the miR-339-5p +PTP4A1 and the miR-con +PTP4A1 groups ( P < 0.05 ). The miR-con +PTP4A1 group had a greater migration rate and invasive index than the miR-con and miR-339-5p +PTP4A1 groups ( P < 0.05 ). Conclusion. MiR-339-5P inhibits angiogenic mimicry, migration, and invasion of brain glioma U251 cells by inhibiting the PTP4A1/HMGB1 signal pathway.

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“…Evidence has demonstrated that several miRNAs influence the neurobiological function (synaptic plasticity and neurogenesis) which is important for potential diagnosis and treatment of drug abuse. 10 - 12 In addition, numerous brain-enriched miRNAs play a critical role in drug abuse through several pathways such as synaptic remodeling and dendritic spine morphogenesis. 9 , 13 Therefore, the development of miRNAs that are associated with drug abuse can be important for management of addiction.…”

Section: Discussionmentioning

confidence: 99%

“…Several neurological mediators are introduced as targets of miR-127 and miR-132 that engage in synaptic transmission, angiogenesis, and inflammation. 10 , 11 …”

Section: Introductionmentioning

confidence: 99%

MicroRNA-127 and MicroRNA-132 Expression in Patients with Methamphetamine Abuse in East Azerbaijan, Iran: A Case-Control Study

Moradali

¹

,

Soltanzadeh

²

,

Montazam

³

et al. 2022

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Background: Addiction is a personal and social problem worldwide, and has physical and psychological effects on consumers’ health. Recently, miRNAs have been described as noninvasive biomarkers. Currently, methamphetamine abuse (MA) is mainly diagnosed by chromatography. This study aimed to investigate the expression and diagnostic value of miR-127 and miR-132 in blood samples of patients with MA and non-user healthy controls. Methods: A total of 60 patients with MA (case group) and 60 non-user healthy individuals (control group) were selected from Tabriz, East Azerbaijan, Iran. Peripheral blood was obtained and total RNA was extracted. Then, cDNA synthesis was performed and miR-127 and miR-132 expression was evaluated using real time polymerase chain reaction (PCR) method. Findings: The results of this study demonstrated that miR-127 was significantly lower (0.042-fold change) in patients with MA than in the control group (P<0.05). However, miR-132 was significantly higher (7.1-fold change) in patients with MA than in the control group (P<0.05). Conclusion: In general, expression of miR-127 and miR-132 may alter in patients with MA. Further studies are needed to identify underlying molecular mechanisms in patients with MA.

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“…Evidence suggested that the expression of the several miRNAs in the brain may be involved in memory formation, neuronal morphogenesis, and drug addiction [6,7]. Previous studies provided that miRNAs are involved in the pathogenesis of drugs addiction, such as alcohol, nicotine, cocaine, and other drugs [8,9].…”

Section: Introductionmentioning

confidence: 99%

Investigation of miR-222 as a potential biomarker in diagnosis of patients with methamphetamine abuse disorder

Fathi

¹

,

Soltanzadeh

²

,

Tanomand

³

et al. 2022

Egypt J Med Hum Genet

1

0

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Background Methamphetamine abuse disorder is an important social and health problem worldwide. Diagnosis and confirmation of patients with methamphetamine abuse using serum are important in many fields. MicroRNAs (miRNAs) are small non-coding oligonucleotides and recently suggested as a biomarker for earlier diagnosis of several human disorders. Therefore, in this study, we investigated miR-222 and miR-212 expressions in blood of patients with methamphetamine abuse disorder comparison with healthy control subjects. Results The results revealed that the expression of blood miR-222 is significantly increased (12.9-fold change) in patients with methamphetamine abuse disorders compared to healthy controls (p < 0.05). However, expression of miR-212 is at the same levels in both patients and healthy controls (p > 0.05). Conclusions In general, we suggested that the miR-222 may play a potentially important role in pathogenesis of methamphetamine abuse disorder and can be considered as an applied tool for identifying individuals with methamphetamine abuse disorder.

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Potential Therapeutic Effects of Melatonin Mediate via miRNAs in Cancer

Cited by 17 publications

References 147 publications

Inhibitory Effect of miR-339-5p on Glioma through PTP4A1/HMGB1 Pathway

Inhibitory Effect of miR-339-5p on Glioma through PTP4A1/HMGB1 Pathway

MicroRNA-127 and MicroRNA-132 Expression in Patients with Methamphetamine Abuse in East Azerbaijan, Iran: A Case-Control Study

Investigation of miR-222 as a potential biomarker in diagnosis of patients with methamphetamine abuse disorder

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