Potential testicular toxicity of sodium nitrate in adult rats

Aly, Hamdy A.A.; Mansour, Ahmed M.; Abo-Salem, Osama M.; Abd-Ellah, Hala F.; Abdel-Naim, Ashraf B.

doi:10.1016/j.fct.2009.11.034

Cited by 38 publications

(27 citation statements)

References 44 publications

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“…Our results showed that nitrate or insulin administration to diabetic rats ameliorated the histopathological alterations towards control rats. Unlike our finding, it has been shown that sodium nitrate treatment at doses of 100–200 mg kg −1 for 60 days caused histopathological alterations in rat testis (Aly, Mansour, Abo‐Salem, Abd‐Ellah, & Abdel‐Naim, ). However, another study has shown no histopathological disorders following nitrate administration (90, 200, 500 and 700 ppm) to control mice for 35 days (Pant & Sirivastava, 2002).…”

Section: Discussioncontrasting

confidence: 99%

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

Oghbaei

Alipour

Hamidian

et al. 2018

Experimental Physiology

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Inorganic nitrate supplementation is a possible therapeutic agent in diabetes. The aim of this study was to evaluate the effect of nitrate on the reproductive system in streptozotocin-induced diabetic male rats. Fifty male Wistar rats were allocated randomly to five groups: control (C), control plus nitrate (CN), diabetic (D), diabetic plus insulin (DI) and diabetic plus nitrate (DN). Sodium nitrate was administered for 2 months in the drinking water (100 mg l ) of the CN and DN groups. Insulin was injected at 2-4 U daily in the DI group. Serum glucose level and body weight were measured at the beginning of the study and at regular intervals. At the end of the study, serum levels of glucose, insulin, nitrogen oxides (NOx), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone were assessed as well as sperm parameters, testis morphometry and histology, and testicular miR-34b and p53 mRNA expression. Nitrate treatment in diabetic rats significantly improved sperm parameters, epididymal weight, spermatogenesis and testicular histology as well as decreasing serum glucose and testicular p53 gene and miR-34b expression, although it had no effect on serum LH and FSH levels. In diabetic rats, serum insulin and NOx, body weight, testicular and epididymal weight, sperm count and motility, testis morphology, spermatogenesis indices, Johnsen's score, and testosterone were significantly lower than in controls. Nitrate administration increased serum insulin, NOx and testosterone levels in the DN group. Consuming water supplemented with sodium nitrate could improve diabetes-induced testicular functional and structural disorders; these favourable effects may be related to increased serum insulin and decreased serum glucose, as well as modulation of apoptosis in testis.

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Section: Discussioncontrasting

confidence: 99%

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

Oghbaei

Alipour

Hamidian

et al. 2018

Experimental Physiology

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“…Several reports have linked its protective action to anti-inflammatory and anti-infectious activities of this plant (Srinivas, et al,1992). The protective effect of curcumin may be explained by the fact that it prevents cellular damage occurring as a result of oxidative stress (Aly et al, 2009). In the present study, microscopic examination of the kidney of rats treated with sodium nitrite after five weeks showed alterations of kidney parenchyma.…”

Section: Disscussionmentioning

confidence: 48%

Comparative Studies on Turmeric and Vitamin C on Sodium Nitrite treated Rats

Hassan¹,

Zaglool²,

El-shamy³

2018

AJVS

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Key words:Sodium nitrite, Turmeric, Vitamin C, Lipid peroxidation, Superoxidedismutase, Glutathione reductase,The current study was performed to investigate the comparative effect of Turmeric and vitamin C on the oxidative damage caused by sodium nitrite in rats. Sixty albino rats were used, divided into four equal groups, group (1) considered as a control group, group (2) received sodium nitrite (NaNO 2 ) 30mg/kg.bw., group (3) received (NaNO2) 30mg/kg.bw. and vitamin C 200 mg /kg bw., group (4) received (NaNO 2 ) 30mg/kg.bw. and turmeric 0.5g/ kg.bw. Animals were daily treated for five weeks. Sodium nitrite treated Rats showed a microcytic hypochromic anemia , significant increase (p ≤ 0.05) in total leukocytes (WBCs), total billirubin , lipid peroxidase(LPO), superoxidedismutase (SOD), significant decrease in glutathione reductase (GR), globulin ( β, γ globulin) and vitamin C value as compared to control group. (NaNO 2 ) with Vitamin C treated Rats showed significant increase in RBCs count, HB value, globulin (β, α) and vitamin C value ,significant decrease in total billirubin, (LPO)and (SOD)as compared to (NaNO 2 ) treated group. (NaNO 2 ) with turmeric treated Rats showed slight ameliorating effect through significant increase in RBCs count but hemoglobin value was declined, significant decrease in total billirubin, (LPO)and (SOD), significant increase in globulin ( γ globulin) and (GR) as compared to (NaNO 2 ) treated group. Histopathological changes of sodium nitrite treated rats after five weeks exhibited cytoplasmic vacuolization of hepatocytes, dilated congested veins and mononuclear cells infiltration. The damage also extended to kidney represented by shrinkage glomerular tuft, distention of Bowman's space, degeneration of lining tubular epithelial, The results declared that vitamin C preceded the turmeric in ameliorate the toxic effect of sodium nitrite on rats.

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“…Aly et al showed that treatment of animals with 100 to 200 mg/kg B.W with sodium nitrite for 60 days caused a significant reduction in rat testicular weight and along with histopathological changes including atrophy, piknosis, and spermatogenesis disorder. They concluded that chronic exposure to sodium nitrate could damage the testicles and these effects are probably due to oxidative stress [9]. Panet et al…”

Section: Discussionmentioning

confidence: 99%

“…NO, which is a metabolite of nitrite, has a dual role in the synthesis of testosterone in culture media [8]. The concentration of nitrite in water should not be exceed 3 mg/L (ion) otherwise, it may cause oxidative stress associated with male infertility [9,10]. On the other hand, some studies demonstrated that ECM proteins can modulate steroidogenesis in vitro [11].…”

Section: Introductionmentioning

confidence: 99%

Effect of chronic sodium nitrite administration on the expression of fibronectin in interstitial tissue of mice testis: An immunohistochemical study

Amini¹,

Nikravesh²,

Jalali³

et al. 2018

biomedicalresearch

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Background and Objectives: Nitrite and nitrate as common derivatives of nitrogen distribute throughout the body after consumption which consequently increases the content of their metabolites such as nitric oxide in biological fluids. High concentrations of nitrite and nitrate affect the fertility of men by changing the parameters of sperm. Half of the infertility problems are related to men. No study has been conducted on the possible effects of water contaminants on the expression of fibronectin in testicular interstitial tissue. Therefore, the present study aims to investigate the effects of chronic administration of sodium nitrite on the expression of fibronectin in interstitial tissue of mice testis using immunohistochemical assessments. Methods: This was an animal laboratory study conducted on male mice (n=24) who were divided into three groups: a control group and two treatment groups I and II. For 60 consecutive days, the control group received water containing no additives, and the treatment groups I and II water containing 3 and 50 mg/L of sodium nitrite, respectively. After sacrificing the animals, their testes were removed from both sides, and the right testicle was used for the immunohistochemical study and the left for real time polymerase chain reaction (RT-PCR) assessment. Results: The amount of fibronectin mRNA was 0.92 fold in treatment group I and 0.8 fold in treatment group II, which showed no significant differences between the groups. Moreover, the qualitative expression of this protein in the interstitial tissue reduced insignificantly in treatment group II compared to the other groups (p=0.094). Conclusion: Consuming water contaminated with sodium nitrite could not affect spermatogenesis through changing the expression of fibronectin in the interstitium of testis.

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Potential testicular toxicity of sodium nitrate in adult rats

Cited by 38 publications

References 44 publications

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

Comparative Studies on Turmeric and Vitamin C on Sodium Nitrite treated Rats

Effect of chronic sodium nitrite administration on the expression of fibronectin in interstitial tissue of mice testis: An immunohistochemical study

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