Potential Suppressive Effect of Nicotine on the Inflammatory Response in Oral Epithelial Cells: An In Vitro Study

An, Na; Holl, Jasmin; Wang, Xuekui; Rausch, Marco Aoqi; Andrukhov, Oleh; Rausch‐Fan, Xiaohui

doi:10.3390/ijerph18020483

Cited by 12 publications

(3 citation statements)

References 71 publications

(43 reference statements)

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“…Nicotine is a common ingredient in tobacco and e-cigarettes, and it is present in tobacco and most e-cigarettes. Studies have shown that nicotine may inhibit the production of inflammatory factors in oral epithelial cells, while other studies have shown that nicotine can also be produce cytotoxicity to human periodontal cells and induce human periodontal cells to produce IL-1β and IL-8 . E-cigarettes are cytotoxic to a variety of cells, including gingival fibroblasts, gingival mesenchymal stem cells, and gingival epithelial cells, and affect cell proliferation, but e-cigarettes have less effect on cell proliferation and viability than tobacco .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on Human Gingival Epithelial Cells

Liu

Yue

et al. 2023

ACS Omega

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Smoking increases the risk of a number of diseases, including cardiovascular, oral, and lung diseases. E-cigarettes are gaining popularity among young people as an alternative to cigarettes, but there is debate over whether they are less harmful to the mouth than e-cigarettes. In this study, human gingival epithelial cells (HGECs) were treated with four commercially available e-cigarette aerosol condensates (ECAC) or commercially available generic cigarette smoke condensates (CSC) with different nicotine concentrations. Cell viability was determined by MTT assay. Cell apoptosis was observed by acridine orange (AO) and Hoechst33258 staining. The levels of type I collagen, matrix metalloproteinase (MMP-1, MMP-3), cyclooxygenase 2, and inflammatory factors were detected by ELISA and RT-PCR. Finally, ROS levels were analyzed by ROS staining. The different effects of CSC and ECAC on HGECs were compared. The results showed that higher nicotine concentration of CS significantly reduced the activity of HGECs. By contrast, all ECAC had no significant effect. The levels of matrix metalloproteinase, COX-2, and inflammatory factors were higher in HGECs treated with CSC than those treated with ECAC. In contrast, the level of type I collagen was higher in HGECs treated with ECAC than those treated with CSC. In conclusion, all four flavors of e-cigarettes were less toxic to HGE cells than tobacco, but further clinical studies are needed to determine whether e-cigarettes are less harmful to oral health than conventional cigarettes.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on Human Gingival Epithelial Cells

Liu

Yue

et al. 2023

ACS Omega

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“…Nicotine inhibited the expression of β-defensin. Nicotine may have an inhibitory effect on the production of inflammatory factors in oral epithelial cells [9]. Studies have shown that nicotine also restrains the proliferation and cell cycle of human periodontal ligament cells by upregulating miR-30a expression and downregulating cell cycle protein E2 expression, thereby disrupting the regeneration of periodontal tissue [10].…”

Section: Composition Analysis Of Electronic Cigarettes and Traditiona...mentioning

confidence: 99%

Electronic cigarettes, traditional cigarettes, and oral health

Liu

Yue²,

Zhao³

et al. 2022

International Conference on Biomedical and Intelligent Systems (IC-BIS 2022)

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Background: Electronic cigarettes are increasingly preferred by young people today, and most people use them to quit smoking. However, the impact of e-cigarettes on human oral health is still controversial. It is debatable whether e-cigarettes are as safe, regulated, and harmless as one might believe, and whether they have potential side effects on the human body. Objective: In this paper, we reviewed the literature related to traditional cigarettes and e-cigarettes and oral health in recent years, and investigate the impact of e-cigarettes and traditional cigarettes on oral health from four aspects: analysis of the composition of e-cigarettes and traditional cigarettes, effects on oral cells, oral flora, and clinical oral health. Conclusion: Both e-cigarettes and traditional cigarettes have an impact on a person's oral health. However, extensive experimental data is needed to prove whether e-cigarette aerosol is more harmful to the oral cavity than cigarette smoke (CS).

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“…Using microarrays representative of the P. gingivalis genome, it is revealed that CSE-exposure resulted in differential regulation of 6.8% of P. gingivalis genes, including detoxification and oxidative stress-related genes, DNA repair genes and multiple genes related to P. gingivalis virulence [ 61 ]. More studies demonstrated the multiple regulatory effects of smoking on expression or function of virulence factors of P. gingivalis , including the long fimbriae FimA [ 52 , 61 ], capsular polysaccharides [ 52 ], outer membrane proteins RagA and RagB [ 61 ], Kgp and Rgp gingipain [ 62 ] and LPS [ 63 ] ( Figure 1 ).…”

Section: Impact Of Smoking On Pathogenicity Of Periodontopathogensmentioning

confidence: 99%

The Impact of Smoking on Subgingival Plaque and the Development of Periodontitis: A Literature Review

Zhang

Dou

et al. 2021

Front. Oral. Health

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Smoking seriously affects oral health and causes a variety of oral diseases. Numerous clinical data show that smoking significantly increases the risk of periodontitis, and the duration and amount of smoking are positively correlated with the severity of periodontitis. In fact, smoking creates an environment conducive to the colonization of periodontopathogens, which affects the process of periodontitis. Since subgingival plaque which harbors periodontopathogens is the initiation factor of periodontitis, it is critical to study the impact of smoking on subgingival microbiota for understanding the relationship between smoking and periodontitis. Continuous advances have been made on the understanding of effects of smoking on subgingival plaque and the development of periodontitis. Smoking is observed to enhance the pathogenicity of periodontopathogens, especially the red complex microorganisms, via promoting their colonization and infection, and regulating the expression and function of multiple virulence factors. Furthermore, smoking has a negative impact on periodontal microecological homeostasis, which is reflected in the decrease of commensal bacteria and the increase of periodontopathogens, as well as the changes in the interaction between periodontopathogens and their commensal microbes in subgingival biofilm, thus influencing the pathogenicity of the subgingival plaque. In summary, the mechanism of smoking on subgingival plaque microorganisms represented by the red complex and its effect on the periodontal microecology still need to be further explored. The relevant research results are of great significance for guiding the periodontal clinical treatment of smoking population. This review summarizes the effects and relevant mechanisms of smoking on subgingival plaque and the development of periodontitis.

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Potential Suppressive Effect of Nicotine on the Inflammatory Response in Oral Epithelial Cells: An In Vitro Study

Cited by 12 publications

References 71 publications

Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on Human Gingival Epithelial Cells

Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on Human Gingival Epithelial Cells

Electronic cigarettes, traditional cigarettes, and oral health

The Impact of Smoking on Subgingival Plaque and the Development of Periodontitis: A Literature Review

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