Growth of Zea mays L. cv Potro roots was inhibited by the herbicide metsulfuron methyl (MSM) at the lowest concentration tested: 5 nanomoles per liter. Pretreatment of corn seeds with commercial 1,8-naphthalic anhydride (NA) at 1% (w/w) partially reversed MSM-induced root growth inhibition. MSM at a concentration of 52 nanomoles per liter was taken up rapidly by roots and accumulated in the corn tissue to concentrations three times those in the extemal medium; the safener NA increased MSM uptake up to 48 hours. The protective effect of NA was related to the ability of the safener to increase the metabolism of MSM; tenfold increases in the metabolic rates of MSM were observed in NA-pretreated corn seedlings grown for 48 hours on 52 nanomolar[14C]MSM solution. DNA synthesis determined by measurement of [3H]thymidine incorporation into DNA was inhibited by root MSM applications; after a 6-hour application period, 13 nanomolar MSM solution reduced DNA synthesis by 64%, and the same reduction was also observed with NA-pretreated seedlings. Pretreatment of corn seeds with safener NA did not increase the acetolactate synthase activity in the roots and did not change, up to 13 micromoles per liter, the in vitro sensitivity of roots to MSM.MSM' is the active ingredient in Du Pont "Ally"' weed killer. This new sulfonylurea herbicide is active on most vascular plants and is used for weed control in small grains. An important feature of this compound is its high herbicidal activity at extremely low application rates; recommended amounts for weed control in wheat are between 6 and 8 g ai/ha. 'Abbreviations: MSM, metsulfuron methyl (methyl 2-[(I[(4-methoxy-6-methyl-1,3,5-triazin-2-yl) amino] carbonyl} amino) sulfonyl] benzoate); ai, active ingredient; ALS, acetolactate synthase, used in the more general sense of acetohydroxyacid synthase;