Potential roles of hsa_circ_000839 and hsa_circ_0005986 in breast cancer

Abstract

Background Breast cancer (BC) is one of the leading causes of death among women around the world. Circular RNAs (circRNAs) are a newly discovered group of non‐coding RNAs that their roles are being investigated in BC and other cancer types. In this study, we evaluated the association of hsa_circ_0005986 and hsa_circ_000839 in tumor and adjacent normal tissues of BC patients with their clinicopathological characteristics. Materials and methods Total RNA was extracted from tumors and adjacent non‐tumor tissues by the Trizol isolation reagent, and cDNA was synthesized using First Strand cDNA Synthesis Kit (Thermo Scientific). The expression level of hsa_circ_0005986 and hsa_circ_000839 was quantified using RT‐qPCR. Online in silico tools were used for identifying potentially important competing endogenous RNA (ceRNA) networks of these two circRNAs. Results The expression level of hsa_circ_0005986 and hsa_circ_000839 was lower in the tumor as compared to adjacent tissues. The expression level of hsa_circ_0005986 in the patients who had used hair dye in the last 5 years was significantly lower. Moreover, a statistically significant negative correlation between body mass index (BMI) and hsa_circ_000839 expression was observed. In silico analysis of the ceRNA network of these circRNAs revealed mRNAs and miRNAs with crucial roles in BC. Conclusion Downregulation of hsa_circ_000839 and hsa_circ_0005986 in BC tumors suggests a tumor‐suppressive role for these circRNAs in BC, meriting the need for more experimentations to delineate the exact mechanism of their involvement in BC pathogenesis.

Epigenetic modifications in obesity‐associated diseases

“…189 Evidence suggests that downregulated hsa_circ_000839 in patients with BC exhibits a negative correlation with BMI, but the underlying mechanism is still unclear. 190 A transcriptome study of circRNAs was conducted, focusing on obese and postmenopausal patients with EC and revealed the presence of 174 differentially expressed circRNA in EC tissues compared with adjacent normal tissues. 191 Nonetheless, additional functional research is required to determine the circRNAs that can offer diagnostic and prognostic assistance in relation to obesityrelated EC.…”

Hsa_circ_0013561 promotes progression of nasopharyngeal carcinoma by activating JAK2/STAT3 signaling pathway

“…Circular RNAs (circRNAs) are a special class of non-coding RNA molecules that are formed by a non-canonical splicing mechanism of reverse splicing, connecting the 3' end of the exon to the 5' end of the same or upstream exon, forming a closed loop. 7 At present, domestic and foreign studies have confirmed that circRNA can participate in the occurrence and development of laryngeal squamous cell carcinoma, 8 bladder cancer, 9 breast cancer, 10 etc. In Non-Small Cell Lung Cancer (NSCLC), highly expressed circ_ZNF124 promotes the occurrence and development of NSCLC by promoting the activation of JAK2/STAT3 signaling pathway.…”

Evaluation of the Expression of Infection-Related Long Noncoding RNAs among COVID-19 Patients: A Case-Control Study

“…Tese RNAs are categorized into two groups as follows: short ncRNAs with a length <200 nucleotides and long noncoding RNAs (lncRNAs) with a length over 200 nucleotides [13]. Although most of the lncRNAs are not protein-coding, they are involved in modulation of transcription patterns and regulation of diferent protein activities [13][14][15][16]. LncRNAs have a crucial role in infammatory pathways and infammasomes [17].…”