Potential Roles for Two Human Homeodomain Containing Proteins in the Proliferation and Differentiation of Human Hematopoietic Progenitors

Kehrl, John H.; Deguchi, Yasuo

doi:10.3109/10428199309145879

Cited by 16 publications

(19 citation statements)

References 18 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both genes share terms such as bone, marrow, lineage, hemopoietic, and CD34, suggesting their involvement in hemopoiesis. Indeed, both Hlx and Hoxc4 are expressed in myeloid and lymphoid cells at various stages of development (63,64). Hlx is also involved in the signaling pathway that leads to type 1 cytokine production in T cells (65) and plays an important role in T cell development (66), suggesting that it could similarly regulate DC lineage determination.…”

Section: Discussionmentioning

confidence: 99%

Relationships Among Murine CD11chigh Dendritic Cell Subsets as Revealed by Baseline Gene Expression Patterns

Edwards

Chaussabel

Tomlinson

et al. 2003

The Journal of Immunology

120

119

View full text Add to dashboard Cite

The functional relationships and properties of different subtypes of dendritic cells (DC) remain largely undefined. To better characterize these cells, we used global gene analysis to determine gene expression patterns among murine CD11chigh DC subsets. CD4+, CD8α+, and CD8α− CD4− (double negative (DN)) DC were purified from spleens of normal C57/BL6 mice and analyzed using Affymetrix microarrays. The CD4+ and CD8α+ DC subsets showed distinct basal expression profiles differing by >200 individual genes. These included known DC subset markers as well as previously unrecognized, differentially expressed CD Ags such as CD1d, CD5, CD22, and CD72. Flow cytometric analysis confirmed differential expression in nine of nine cases, thereby validating the microarray analysis. Interestingly, the microarray expression profiles for DN cells strongly resembled those of CD4+ DC, differing from them by <25 genes. This suggests that CD4+ and DN DC are closely related phylogenetically, whereas CD8α+ DC represent a more distant lineage, supporting the historical distinction between CD8α+ and CD8α− DC. However, staining patterns revealed that in contrast to CD4+ DC, the DN subset is heterogeneous and comprises at least two subpopulations. Gene Ontology and literature mining analyses of genes expressed differentially among DC subsets indicated strong associations with immune response parameters as well as cell differentiation and signaling. Such associations offer clues to possible unique functions of the CD11chigh DC subsets that to date have been difficult to define as rigid distinctions.

show abstract

Section: Discussionmentioning

confidence: 99%

Relationships Among Murine CD11chigh Dendritic Cell Subsets as Revealed by Baseline Gene Expression Patterns

Edwards

Chaussabel

Tomlinson

et al. 2003

The Journal of Immunology

120

119

View full text Add to dashboard Cite

show abstract

“…Moreover, HLX inactivation reduces the levels of c-myc, c-fos, cyclin B, and p34 cdc2 mRNA expression, 24 which are cell cycle regulator genes implicated in trophoblast cell function. 25 Therefore, c-myc, c-fos, cyclin B, and p34 cdc2 were the first identified targets of HLX.…”

Section: Hlx Inactivation Impairs Cd34mentioning

confidence: 99%

“…Furthermore, the PCR array chosen in this study contained several cell cycle regulatory genes, including CCNB1, MYC, CDKN1C, and JUN, which have been previously identified to be the targets of HLX in hematopoietic progenitor cells. 24 Procedures were performed according to the manufacturer's protocol. Briefly, diluted cDNA prepared using the RT 2 First-Strand kit (as described above) was added to a master mix containing the fluorescent SYBR green/ ROX dye.…”

Section: Pcr Arraymentioning

confidence: 99%

See 1 more Smart Citation

Downstream Targets of Homeobox Gene HLX Show Altered Expression in Human Idiopathic Fetal Growth Restriction

Rajaraman

Murthi

Pathirage

et al. 2010

The American Journal of Pathology

View full text Add to dashboard Cite

Fetal growth restriction (FGR) , a clinically significant pregnancy disorder , is poorly understood at the molecular level. This study investigates idiopathic FGR associated with placental insufficiency. Previously, we showed that the homeobox gene HLX is expressed in placental trophoblast cells and that HLX expression is significantly decreased in human idiopathic FGR. Here , we used the novel approach of identifying downstream targets of HLX in cell culture to detect potentially important genes involved in idiopathic FGR. Downstream targets were revealed by decreasing HLX expression in cultured trophoblast cells with HLX-specific small interfering RNAs to model human idiopathic FGR and comparing these levels with controls using a real-time PCR-based gene profiling system. Changes in candidate HLX target mRNA levels were verified in an independent trophoblast cell line, and candidate target gene expression was assessed in human idiopathic FGR-affected placentae (n ‫؍‬ 25) compared with gestation-matched controls (n ‫؍‬ 25). The downstream targets RB1 and MYC , cell cycle regulatory genes , showed significantly increased mRNA levels in FGR-affected tissues compared with gestation-matched controls , whereas CCNB1 , ELK1 , JUN , and CDKN1 showed significantly decreased mRNA levels (n ‫؍‬ 25 , P < 0.001 , t-test). The changes for RB1 and CDKN1C were verified by Western blot analysis in FGR-affected placentae compared with gestation-matched controls (n ‫؍‬ 6). We conclude that cell cycle regulatory genes RB1 , MYC , CCNB1 , ELK1 , JUN , and CDKN1C , which control important trophoblast cell functions , are targets of HLX. (Am J

show abstract

“…The homeobox transcription factor HLX1 is essential in haematopoietic development (Kennedy et al, 1994) and there is convincing evidence implicating HLX1 in immature and stem cell biology (Deguchi et al, 1992;Kehrl & Deguchi, 1993). A function of the transcription factor could therefore be to help establish the frequency of stem cells during early development.…”

Section: Hlx1mentioning

confidence: 99%

Advances in the understanding of susceptibility to treatment‐related acute myeloid leukaemia

Seedhouse

Russell

2007

Br J Haematol

View full text Add to dashboard Cite

SummaryTreatment-related acute myeloid leukaemia (t-AML) is a devastating complication following exposure to the cytotoxic and genotoxic agents used to treat a primary malignancy. Whilst the incidence of t-AML is rising, it still only occurs in a minority of patients who have received chemotherapy and/or radiotherapy treatment and hence it is important to identify factors that may confer susceptibility to the development of the condition. This paper reviews the literature and discusses the advances and limitations in our understanding of susceptibility factors to t-AML. In particular, it concentrates upon genetic polymorphisms in detoxification genes and in genes belonging to the major DNA repair pathways. This review also considers more novel susceptibility factors, such as those proposed to determine stem cell number. Increased understanding of t-AML susceptibility may enable steps to be taken to prevent its development and increase the effectiveness of treatment of the disease.

show abstract

Potential Roles for Two Human Homeodomain Containing Proteins in the Proliferation and Differentiation of Human Hematopoietic Progenitors

Cited by 16 publications

References 18 publications

Relationships Among Murine CD11chigh Dendritic Cell Subsets as Revealed by Baseline Gene Expression Patterns

Relationships Among Murine CD11chigh Dendritic Cell Subsets as Revealed by Baseline Gene Expression Patterns

Downstream Targets of Homeobox Gene HLX Show Altered Expression in Human Idiopathic Fetal Growth Restriction

Advances in the understanding of susceptibility to treatment‐related acute myeloid leukaemia

Contact Info

Product

Resources

About