Potential roles for pattern molecule of PAMP-triggered immunity in improving crop cold tolerance

Jin, Yongfeng; Tuang, Za Khai; Wang, Yizhong; Wu, Zhenjiang; Yang, Wei

doi:10.1007/s00299-021-02811-4

Cited by 2 publications

(3 citation statements)

References 34 publications

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“…Moreover, under freezing temperature, much more genes were found to be very highly significantly enriched in very different pathways from cold acclimation (Figure 5). These enriched pathways have also been found involving in responses to freezing stress in previous studies, such as the "MAPK signaling pathwayplant" pathway for cold signal transduction (Guo et al, 2018), the "plant-pathogen interaction" pathway for defense responses (Zarattini et al, 2021;Jin et al, 2022), and the "phenylpropanoid biosynthesis" pathway for the synthesis of important secondary metabolites (Dong and Lin, 2021). Most of the genes significantly enriched in these pathways were up-regulated to the highest levels of expression under freezing temperature in this study (Figure S7).…”

Section: Differences In Genes Associated With Cold Acclimation and Fr...mentioning

confidence: 59%

“…A recent study showed that pathways activated by flg22 could not only help plants defend against pathogen infection, but also induce expression of cold tolerance related genes to alleviate cold injury and enhance cold tolerance (Jin et al, 2022). In plant cells, flg22 is recognized by FLS2, and activates a mitogen-activated protein kinase (MAPK) cascade to promote the expression of downstream related genes (Zipfel et al, 2004), such as WRKY33 and PR1.…”

Section: Important Roles Of Flg22 Ca 2+ and Gibberellin Signal Transd...mentioning

confidence: 99%

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Field plus lab experiments help identify freezing tolerance and associated genes in subtropical evergreen broadleaf trees: A case study of Camellia oleifera

Xie

Zhang²,

Cheng³

et al. 2023

Front. Plant Sci.

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The molecular mechanisms of freezing tolerance are unresolved in the perennial trees that can survive under much lower freezing temperatures than annual herbs. Since natural conditions involve many factors and temperature usually cannot be controlled, field experiments alone cannot directly identify the effects of freezing stress. Lab experiments are insufficient for trees to complete cold acclimation and cannot reflect natural freezing-stress responses. In this study, a new method was proposed using field plus lab experiments to identify freezing tolerance and associated genes in subtropical evergreen broadleaf trees using Camellia oleifera as a case. Cultivated C. oleifera is the dominant woody oil crop in China. Wild C. oleifera at the high-elevation site in Lu Mountain could survive below −30°C, providing a valuable genetic resource for the breeding of freezing tolerance. In the field experiment, air temperature was monitored from autumn to winter on wild C. oleifera at the high-elevation site in Lu Mountain. Leave samples were taken from wild C. oleifera before cold acclimation, during cold acclimation and under freezing temperature. Leaf transcriptome analyses indicated that the gene functions and expression patterns were very different during cold acclimation and under freezing temperature. In the lab experiments, leaves samples from wild C. oleifera after cold acclimation were placed under −10°C in climate chambers. A cultivated C. oleifera variety “Ganwu 1” was used as a control. According to relative conductivity changes of leaves, wild C. oleifera showed more freezing-tolerant than cultivated C. oleifera. Leaf transcriptome analyses showed that the gene expression patterns were very different between wild and cultivated C. oleifera in the lab experiment. Combing transcriptome results in both of the field and lab experiments, the common genes associated with freezing-stress responses were identified. Key genes of the flg22, Ca2+ and gibberellin signal transduction pathways and the lignin biosynthesis pathway may be involved in the freezing-stress responses. Most of the genes had the highest expression levels under freezing temperature in the field experiment and showed higher expression in wild C. oleifera with stronger freezing tolerance in the lab experiment. Our study may help identify freezing tolerance and underlying molecular mechanisms in trees.

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Section: Differences In Genes Associated With Cold Acclimation and Fr...mentioning

confidence: 59%

Section: Important Roles Of Flg22 Ca 2+ and Gibberellin Signal Transd...mentioning

confidence: 99%

Field plus lab experiments help identify freezing tolerance and associated genes in subtropical evergreen broadleaf trees: A case study of Camellia oleifera

Xie

Zhang²,

Cheng³

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Flagellin 22 (flg22), as a conserved molecule pathogen-associated pattern, can trigger innate immune responses in plants [ 58 ]. After flg22 treatment, the accumulation of ROS and callose deposition in Col-WT leaves were continuously increased from 24 h to 72 h, and these responses were even stronger in the HbRPW8-a overexpression lines ( Figure 6 a–d).…”

Section: Resultsmentioning

confidence: 99%

Ectopic Expression of HbRPW8-a from Hevea brasiliensis Improves Arabidopsis thaliana Resistance to Powdery Mildew Fungi (Erysiphe cichoracearum UCSC1)

Liu

et al. 2022

IJMS

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The RPW8s (Resistance to Powdery Mildew 8) are atypical broad-spectrum resistance genes that provide resistance to the powdery mildew fungi. Powdery mildew of rubber tree is one of the serious fungal diseases that affect tree growth and latex production. However, the RPW8 homologs in rubber tree and their role of resistance to powdery mildew remain unclear. In this study, four RPW8 genes, HbRPW8-a, b, c, d, were identified in rubber tree, and phylogenetic analysis showed that HbRPW8-a was clustered with AtRPW8.1 and AtRPW8.2 of Arabidopsis. The HbRPW8-a protein was localized on the plasma membrane and its expression in rubber tree was significantly induced upon powdery mildew infection. Transient expression of HbRPW8-a in tobacco leaves induced plant immune responses, including the accumulation of reactive oxygen species and the deposition of callose in plant cells, which was similar to that induced by AtRPW8.2. Consistently, overexpression of HbRPW8-a in Arabidopsis thaliana enhanced plant resistance to Erysiphe cichoracearum UCSC1 and Pseudomonas syringae pv. tomato DC30000 (PstDC3000). Moreover, such HbRPW8-a mediated resistance to powdery mildew was in a salicylic acid (SA) dependent manner. Taken together, we demonstrated a new RPW8 member in rubber tree, HbRPW8-a, which could potentially contribute the resistance to powdery mildew.

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Potential roles for pattern molecule of PAMP-triggered immunity in improving crop cold tolerance

Cited by 2 publications

References 34 publications

Field plus lab experiments help identify freezing tolerance and associated genes in subtropical evergreen broadleaf trees: A case study of Camellia oleifera

Field plus lab experiments help identify freezing tolerance and associated genes in subtropical evergreen broadleaf trees: A case study of Camellia oleifera

Ectopic Expression of HbRPW8-a from Hevea brasiliensis Improves Arabidopsis thaliana Resistance to Powdery Mildew Fungi (Erysiphe cichoracearum UCSC1)

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