Potential role of salicylic acid in modulating diacylglycerol homeostasis in response to freezing temperatures in <i>Arabidopsis</i>

Tan, Wei‐Juan; Xiao, Shi; Chen, Qinfang

doi:10.1080/15592324.2015.1082698

Cited by 2 publications

(2 citation statements)

References 29 publications

(35 reference statements)

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“…The pedicel of Gr and UD at the full-flowering stage was selected for RNA extracting, subjected to three separate biological replicates and then the transcriptome sequencing was performed using the Illumina platform by BMKCloud, Beijing, China (http://www.biomarker.com.cn (accessed on 8 January 2021) following the manufacturer's instructions. Clean reads were achieved by removing adapters and then mapped to the common buckwheat genome (http://buckwheat.kazusa.or.jp/ (accessed on 8 January 2021)) using Hisat2 [32], and the gene expression levels were estimated by the FPKM (fragments per kilobase of exon per million mapped fragments) method, and DEGs were determined using the criteria FDR ≤ 0.01 and |log2 (Gr_FPKM/UD_FPKM)| ≥ 1. The GO term and KEGG pathway analysis results were considered significant when the Bonferroni (Q-value)-corrected p-value was ≤0.05.…”

Section: Rna Sequencing and Differential Expression Analysismentioning

confidence: 99%

Joint QTL Mapping and Transcriptome Sequencing Analysis Reveal Candidate Seed-Shattering-Related Genes in Common Buckwheat

Chen

Zhang

Liu

et al. 2023

IJMS

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Common buckwheat (Fagopyrum esculentum M.) is an important traditional miscellaneous grain crop. However, seed-shattering is a significant problem in common buckwheat. To investigate the genetic architecture and genetic regulation of seed-shattering in common buckwheat, we constructed a genetic linkage map using the F2 population of Gr (green-flower mutant and shattering resistance) and UD (white flower and susceptible to shattering), which included eight linkage groups with 174 loci, and detected seven QTLs of pedicel strength. RNA-seq analysis of pedicel in two parents revealed 214 differentially expressed genes DEGs that play roles in phenylpropanoid biosynthesis, vitamin B6 metabolism, and flavonoid biosynthesis. Weighted gene co-expression network analysis (WGCNA) was performed and screened out 19 core hub genes. Untargeted GC-MS analysis detected 138 different metabolites and conjoint analysis screened out 11 DEGs, which were significantly associated with differential metabolites. Furthermore, we identified 43 genes in the QTLs, of which six genes had high expression levels in the pedicel of common buckwheat. Finally, 21 candidate genes were screened out based on the above analysis and gene function. Our results provided additional knowledge for the identification and functions of causal candidate genes responsible for the variation in seed-shattering and would be an invaluable resource for the genetic dissection of common buckwheat resistance-shattering molecular breeding.

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Section: Rna Sequencing and Differential Expression Analysismentioning

confidence: 99%

Joint QTL Mapping and Transcriptome Sequencing Analysis Reveal Candidate Seed-Shattering-Related Genes in Common Buckwheat

Chen

Zhang

Liu

et al. 2023

IJMS

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“…The cold stress-induced stimulation of both PLD and PLC/DGK in Arabidopsis cell suspensions, and the expression of separate DGK gene clusters, revealed that DGK genes are upstream of different lipid signaling pathways in plant cold stress responses [162]. While the DGK network operates either simultaneously or as a supplementary mechanism to that of PLDs to trigger PA generation in response to cold stress [65,163].…”

Section: Cold Stressmentioning

confidence: 99%

The Emerging Roles of Diacylglycerol Kinase (DGK) in Plant Stress Tolerance, Growth, and Development

et al. 2020

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Diacylglycerol kinase (DGK) is recognized as the key enzyme of the lipid signaling pathway, which involves the transduction of messages from hormones, neurotransmitters, and immunologic and growth factors. Regarding their essential role in animal physiology, many plant biologists have predicted a similar enzymatic influence in plants. However, a small number of recent studies have revealed the complexity of the involvement of DGK genes in the modulation of plant growth, development, and adaptation in both biotic and abiotic stress conditions. Here, we describe recent discoveries on the role of DGK genes in the plants’ responses to biotic or abiotic stressors. Moreover, we discuss how DGK enzymes regulate plant cellular activities during the adaptation of plants to a readily changing environment. DGK is an enzyme that plays a pivotal role in plant lipid signaling, by catalyzing the phosphorylation of the diacylglycerol (DAG) to phosphatidic acid (PA), which is a crucial molecule in a plant’s metabolic network, leading to its response to various external stresses. DGK enzymes are the principal moderators of PA generation in plant cells; this consequently affects its derived products—hence, enabling their activities in lipid signaling networks and cell homeostasis. Thus, understanding the DGK operational mode and interactions between the production and accumulation of PA would constitute a significant advancement in investigating the mechanism of stress adaptation in plants.

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Potential role of salicylic acid in modulating diacylglycerol homeostasis in response to freezing temperatures in Arabidopsis

Cited by 2 publications

References 29 publications

Joint QTL Mapping and Transcriptome Sequencing Analysis Reveal Candidate Seed-Shattering-Related Genes in Common Buckwheat

Joint QTL Mapping and Transcriptome Sequencing Analysis Reveal Candidate Seed-Shattering-Related Genes in Common Buckwheat

The Emerging Roles of Diacylglycerol Kinase (DGK) in Plant Stress Tolerance, Growth, and Development

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