Potential role of MRN-100, an iron-based compound, in upregulating production of cytokine IL-10 in human dendritic cells to promote an anti-inflammatory response in vitro

Ghoneum, Mamdooh; Gimzewski, James K.; Ghoneum, Aya D; Agrawal, Sudhanshu

doi:10.1177/2058738419844932

Cited by 3 publications

(1 citation statement)

References 44 publications

(61 reference statements)

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“…Our results showed that patients with diabetes presented higher IL-10 and TNF-α gene expression compared to those without diabetes, suggesting an increased inflammatory activity. As HbA1c levels decrease, there is a change in a cytokinetic profile with a decrease in the expression of IL-10, showing a compensatory cytokine response of defense [ 33 ]. Therefore, the cytokine pattern found in this study would be a response to the glycemic damage, varying according to the metabolic state and consequent cell aggression [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression

et al. 2023

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Type 1 diabetes mellitus (T1DM) is one of the major chronic diseases in children worldwide. This study aimed to investigate interleukin-10 (IL-10) gene expression and tumor necrosis factor-alpha (TNF-α) in T1DM. A total of 107 patients were included, 15 were T1DM in ketoacidosis, 30 patients had T1DM and HbA1c ≥ 8%; 32 patients had T1DM and presented HbA1c < 8%; and 30 were controls. The expression of peripheral blood mononuclear cells was performed using the reverse transcriptase–polymerase chain reaction in real time. The cytokines gene expression was higher in patients with T1DM. The IL-10 gene expression increased substantially in patients with ketoacidosis, and there was a positive correlation with HbA1c. A negative correlation was found for IL-10 expression and the age of patients with diabetes, and the time of diagnosis of the disease. There was a positive correlation between TNF-α expression with age. The expression of IL-10 and TNF-α genes showed a significant increase in DM1 patients. Once current T1DM treatment is based on exogenous insulin, there is a need for other therapies, and inflammatory biomarkers could bring new possibilities to the therapeutic approach of the patients.

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Section: Discussionmentioning

confidence: 99%

The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression

et al. 2023

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Therapeutic potential of induced iron depletion using iron chelators in Covid-19

Naseef

Elayadeth-Meethal

et al. 2022

Saudi Journal of Biological Sciences

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Ferritin, which includes twenty-four light and heavy chains in varying proportions in different tissues, is primarily responsible for maintaining the body's iron metabolism. Its normal value is between 10 and 200 ngmL -1 in men and between 30-300 ngmL -1 in women. Iron is delivered to the tissue via them, and they act as immunomodulators, signaling molecules, and inflammatory markers. When ferritin level exceeds 1000 µgL -1 , the patient is categorized as having hyperferritinemia. Iron chelators such as deferiprone, deferirox, and deferoxamine are currently FDA approved to treat iron overload. The inflammation cascade and poor prognosis of COVID-19 may be attributed to high ferritin levels. Critically ill patients can benefit from deferasirox, an iron chelator administered orally at 20-40 mgkg -1 once daily, as well as intravenous deferoxamine at 1000 mg initially followed by 500 mg every 4 to 12 hours. It can be combined with monoclonal antibodies, antioxidants, corticosteroids, and lactoferrin to make iron chelation therapy effective for COVID-19 victims. In this article, we analyze the antiviral and antifibrotic activity of iron chelators, thereby promoting iron depletion therapy as a potentially innovative treatment strategy for COVID-19.

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Anti-radiation effect of MRN-100: a hydro-ferrate fluid, in vivo

Ghoneum,

Badr El-Din,

Alaa El-Dein

2024

Journal of Radiation Research

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Ionizing radiation (IR) severely harms many organs, especially the hematopoietic tissue, mandating the development of protective nutraceuticals. MRN-100, a hydro-ferrate fluid, has been shown to protect γ-radiated fish against hematopoietic tissue damage and lethality. The current study aimed to examine MRN-100’s protective effect against irradiated mice and explore the mechanisms underlying its effect. Mice received a single acute, sub-lethal, 5 Gy, whole body dose of X-ray IR. MRN-100 treatment was administered daily for 2-weeks pre-irradiation until 1-week post-irradiation. Spleen and blood were analysed for oxidative stress, hematological, histological and biochemical parameters. Radiation exposure markedly decreased complete blood count (CBC) parameters including hemoglobin, hematocrit, red blood cells, platelets, white blood cells and lymphocytes, and significantly increased neutrophils. In contrast, MRN-100 supplementation to irradiated mice ameliorated all CBC parameters and protected against DNA damage in both splenic cells and serum. It also had an antioxidant effect, increasing the levels of glutathione, superoxide dismutase, catalase and total antioxidant capacity, which were otherwise decreased by irradiation. MRN-100 intake reduced the oxidative stress biomarker levels of nitric oxide, protein carbonyl, malondialdehyde, reactive oxygen species and 8-hydroxydeoxyguanosine, a marker specific to DNA damage. Furthermore, MRN-100 enhanced serum iron and reversed the radiation-induced elevations of liver enzymes. Finally, MRN-100 protected splenic tissue from irradiation as observed by histology. We conclude that MRN-100 consumption may protect against oxidative stress generated by radiation exposure, suggesting that it may be employed as an adjuvant treatment to prevent radiation’s severe damage to important organs.

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Potential role of MRN-100, an iron-based compound, in upregulating production of cytokine IL-10 in human dendritic cells to promote an anti-inflammatory response in vitro

Cited by 3 publications

References 44 publications

The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression

The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression

Therapeutic potential of induced iron depletion using iron chelators in Covid-19

Anti-radiation effect of MRN-100: a hydro-ferrate fluid, in vivo

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