Potential role of Methoprene-tolerant (Met) in methyl farnesoate-mediated vitellogenesis in the Chinese mitten crab (Eriocheir sinensis)

Li, Xilei; Chen, Tiantian; Han, Yang; Huang, Mengting; Jiang, Hong; Huang, Jiawei; Tao, Minhui; Xu, Ruijuan; Xie, Qiming; Su, Shiping

doi:10.1016/j.cbpb.2020.110524

Cited by 13 publications

(3 citation statements)

References 47 publications

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“…In M. nipponense, as well as other crustaceans [60][61][62], females lay eggs after molting off the exoskeleton under favorable conditions, which means that the reproductive cycle occurs synchronously with the molting cycle. Many genes related to both ecdysteroid-and juvenile hormone signaling have been studied and proved to be involved in reproduction in crustaceans [63][64][65][66]. Thus, our results supported the important role of ecdysteroid and juvenile hormone signaling pathways in the hepatopancreatic regulation of ovarian maturation.…”

Section: Discussionsupporting

confidence: 82%

Hepatopancreas transcriptome analyses provide new insights into the molecular regulatory mechanism of fast ovary maturation in Macrobrachium nipponense

et al. 2022

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Background Macrobrachium nipponense is an economically and ecologically important freshwater prawn that is widely farmed in China. In contrast to other species of marine shrimp, M. nipponense has a short sexual maturity period, resulting in not only high stocking densities, but also a reduced survival rate and increased risk of hypoxia. Therefore, there is an urgent need to study the molecular mechanisms underlying fast ovary maturation in this species. Results Comparative transcriptome analysis was performed using hepatopancreatic tissue from female M. nipponense across five ovarian maturation stages to explore differentially expressed genes and pathways involved in ovarian maturation. In total, 118.01 Gb of data were generated from 15 transcriptomes. Approximately 90.46% of clean reads were mapped from the M. nipponense reference genome. A comprehensive comparative analysis between successive ovarian maturation stages generated 230–5814 differentially expressed genes. Gene Ontology (GO) enrichment was highly concentrated in the “biological process” category in all four comparison groups, and mainly focused on energy synthesis and accumulation, energy decomposition and transport. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment results showed that, among 20 significantly enriched KEGG pathways, nine were involved in the synthesis, degradation, and metabolism of carbohydrates, lipids, and other nutrient intermediates, suggesting that the hepatopancreas has an important role in energy supply during ovarian maturation. Furthermore, the “Insect hormone biosynthesis” pathway was found to have a dominant role in the development of the ovary from immaturity to maturity, supporting the hypothesis that ecdysteroid- and juvenile hormone-signaling pathways have an important role in hepatopancreas regulation of ovarian maturation. Conclusion Taken together, this study sheds light on the role of the hepatopancreas in the molecular regulation of ovary maturation in M. nipponense. The present study provided new insights for understanding the mechanisms of reproductive regulation in crustaceans.

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Section: Discussionsupporting

confidence: 82%

Hepatopancreas transcriptome analyses provide new insights into the molecular regulatory mechanism of fast ovary maturation in Macrobrachium nipponense

et al. 2022

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“…One of the endocrine factors is methyl farnesoate (MF), a sesquiterpenoid hormone that is secreted by mandibular organs (51). Previous research has shown that MF can stimulate ovarian maturation and vitellogenesis in crustaceans (52). In this study, a dietary krill oil was more effective than soybean lecithin and egg yolk lecithin in promoting the secretion of MF in L. vannamei, thereby promoting ovarian maturation and vitellogenesis.…”

Section: Discussionmentioning

confidence: 78%

Effects and Mechanism of Different Phospholipid Diets on Ovary Development in Female Broodstock Pacific White Shrimp, Litopenaeus vannamei

et al. 2022

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“…Differences in RXR isoforms mostly correspond to insertions in the DNA-binding and ligand-binding domains for Crustacea, while in insects they are generally located in the amino-terminal activating region (Techa & Chung, 2013;Eichner et al, 2015;Cheong et al, 2015;Hyde et al, 2019a). Over time, Met has been isolated in other insect model species as well as branchiopods and malacostracans (Jindra et al, 2015;Hyde et al, 2019a;Belles, 2019;Truman, 2019;Li et al, 2021b), but efforts to better characterise Met in other arthropods remain rare. Of note, even though insects and crustaceans use different sesquiterpenoid molecules as the master moulting regulator, JH and MF respectively (Figure 3), they are believed to target the same receptor.…”

Section: Factors Involved In Hormonal Stimulation Responsesmentioning

confidence: 99%

The moulting arthropod: a complete genetic toolkit review

Campli,

Volovych,

Kim

et al. 2024

Preprint

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Exoskeletons are a defining character of all arthropods that provide physical support for their segmented bodies and appendages as well as protection from the environment and predation. This ubiquitous yet evolutionarily variable feature has been instrumental in facilitating the adoption of a variety of lifestyles and the exploitation of ecological niches across all environments. Throughout the radiation of Arthropoda that produced the more than one million described modern species, the adaptability afforded by segmentation and exoskeletons has led to a diversity that is unrivalled amongst animals. However, because exoskeletons cannot grow, they must be periodically shed and replaced with new larger ones to accommodate the growing individuals encased within. Arthropods must therefore undergo periodic moulting events, which follow a series of steps from the preparatory pre-moult phase to ecdysis itself and the post-moult maturation of the new exoskeleton. Each event represents a particularly vulnerable period in an arthropod’s life cycle, so the process must be tightly regulated and meticulously executed to ensure successful transitions for normal growth and development. Decades of research in representative arthropods provide a foundation of understanding of the mechanisms involved. Building on this, studies continue to develop and test hypotheses on the presence and function of molecular components, including neuropeptides, hormones, and receptors, as well as the so-called early, late, and fate genes, across arthropod diversity. Here, we review the literature to develop a comprehensive overview of the current status of accumulated knowledge of the genetic toolkit governing arthropod moulting. From the biosynthesis and regulation of ecdysteroid and sesquiterpenoid hormones, to the factors involved in hormonal stimulation responses and exoskeleton remodelling, we identify commonalities and differences, as well as highlighting major knowledge gaps, across arthropod groups. We examine the available evidence supporting current models of how components operate together to prepare for, execute, and recover from ecdysis, comparing reports from Chelicerata, Myriapoda, Crustacea, and Hexapoda. Evidence is generally highly taxonomically imbalanced, with most reports based on insect study systems. Biases are also evident in the research on different moulting phases and processes, with the early triggers and the late effectors generally being the least well explored. Our synthesis contrasts knowledge based on reported observations with reasonably plausible assumptions given current taxonomic sampling, and exposes weak assumptions or major gaps that need addressing. Encouragingly, advances in genomics are driving a diversification of tractable study systems by facilitating the cataloguing of putative genetic toolkits in previously under-explored taxa. Analysis of genome and transcriptome data supported by experimental investigations have validated the presence of an “ultra-conserved” core of arthropod moulting genes. The molecular machinery has likely evolved with elaborations on this conserved pathway backbone, but more taxonomic exploration is needed to characterise lineage-specific changes and novelties. Furthermore, linking these to transformative innovations in moulting processes across Arthropoda remains hampered by knowledge gaps and hypotheses based on untested assumptions. Promisingly however, emerging from the synthesis is a framework that highlights research avenues from the underlying genetics to the dynamic molecular biology through to the complex physiology of moulting.

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Potential role of Methoprene-tolerant (Met) in methyl farnesoate-mediated vitellogenesis in the Chinese mitten crab (Eriocheir sinensis)

Cited by 13 publications

References 47 publications

Hepatopancreas transcriptome analyses provide new insights into the molecular regulatory mechanism of fast ovary maturation in Macrobrachium nipponense

Hepatopancreas transcriptome analyses provide new insights into the molecular regulatory mechanism of fast ovary maturation in Macrobrachium nipponense

Effects and Mechanism of Different Phospholipid Diets on Ovary Development in Female Broodstock Pacific White Shrimp, Litopenaeus vannamei

The moulting arthropod: a complete genetic toolkit review

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