Potential role of intraspecific and interspecific cloning in the conservation of wild mammals

Borges, Alana Azevedo; Pereira, Alexsandra Fernandes

doi:10.1017/s0967199419000170

Cited by 20 publications

(8 citation statements)

References 57 publications

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“…2020 ). Oocyte maturation is required prior to fusion, with the optimum conditions varying between species ( Borges & Pereira 2019 ). Following artificial activation, for example, with ionomycin and 6-dimethylaminopurine, which has been successful in many different species including bovines ( Bhak et al 2006 ), camelids ( Wani et al 2017 ), porcines ( Borges et al 2020 ) and primates ( Liu et al 2018 ), the egg develops to an early embryo in vitro and is then implanted into the uterus of suitable recipient female.…”

Section: Somatic Cell Cryopreservation and Advanced Assisted Reproduc...mentioning

confidence: 99%

Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking

Bolton¹,

Mooney²,

Pettit

et al. 2022

Reproduction and Fertility

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Biodiversity: the variety of all living organisms on Earth is essential for human survival. However, anthropogenic activities are causing the sixth mass extinction, threatening even our own species. For many animals, dwindling numbers are becoming fragmented populations with low genetic diversity, threatening long-term species viability. With extinction rates 1,000-10,000 times greater than is natural, ex situ and in situ conservation programmes need additional support to save species. The indefinite storage of cryopreserved (-196oC) viable cells and tissues (cryobanking), followed by assisted or advanced assisted reproductive technology (ART: utilisation of oocytes and spermatozoa to generate offspring; aART: utilisation of somatic cell genetic material to generate offspring), may be the only hope for species long-term survival. As such, cryobanking should be considered a necessity for all future conservation strategies. Following cryopreservation, ART/aART can be used to reinstate lost genetics back into a population, resurrecting biodiversity. However, for this to be successful, species-specific protocol optimisation and increased knowledge of basic biology for many taxa is required. Current ART/aART is primarily focused on mammalian taxa, however, this needs to be extended to all, including to some of the most endangered: amphibians. Gamete, reproductive tissue and somatic cell cryobanking can fill the gap between losing genetic diversity today, and future technological developments. This review explores species prioritisation for cryobanking and the successes and challenges of cryopreservation and multiple ARTs/aARTs. We discuss the value of cryobanking before more species are lost and the potential of advanced reproductive technologies not only to halt, but reverse biodiversity loss.

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Section: Somatic Cell Cryopreservation and Advanced Assisted Reproduc...mentioning

confidence: 99%

Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking

Bolton¹,

Mooney²,

Pettit

et al. 2022

Reproduction and Fertility

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“…This mismatch of species results in greater inefficiencies still. To date, only a few attempts at iSCNT have been successful at producing healthy clones (Borges and Pereira 2019). iSCNT is a useful and often used experimental design in developmental biology and biomedicine, with rapid strides being made at overcoming these inefficiencies and developmental mismatches.…”

Section: Cloning In Animalsmentioning

confidence: 99%

New developments in the field of genomic technologies and their relevance to conservation management

et al. 2021

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Recent technological advances in the field of genomics offer conservation managers and practitioners new tools to explore for conservation applications. Many of these tools are well developed and used by other life science fields, while others are still in development. Considering these technological possibilities, choosing the right tool(s) from the toolbox is crucial and can pose a challenging task. With this in mind, we strive to inspire, inform and illuminate managers and practitioners on how conservation efforts can benefit from the current genomic and biotechnological revolution. With inspirational case studies we show how new technologies can help resolve some of the main conservation challenges, while also informing how implementable the different technologies are. We here focus specifically on small population management, highlight the potential for genetic rescue, and discuss the opportunities in the field of gene editing to help with adaptation to changing environments. In addition, we delineate potential applications of gene drives for controlling invasive species. We illuminate that the genomic toolbox offers added benefit to conservation efforts, but also comes with limitations for the use of these novel emerging techniques.

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“…(2007), in more than a quarter of iSCNT studies, rabbit oocytes have been used as recipients for donor nuclei from multiple species (i.e., cat, ibex, camel, antelope, human). Whereas rabbit oocytes have been still frequently exploited (Lagutina et al., 2011, 2013; Yamochi et al., 2013; Yelisetti et al., 2016), bovine oocytes are also considered attractive surrogate sources of oocyte and egg cytoplasm for iSCNT (Borges & Pereira, 2019; Dominko et al., 1999; Hua et al., 2008; Lagutina et al., 2011; Lorthongpanich et al., 2008; Tecirlioglu et al., 2006). Both species oocytes demonstrated availability as well as their capacity to reprogramme somatic cells and support pre‐implantation development of embryos derived from nuclei of several species (Borges & Pereira, 2019; Chung et al., 2009; Cordova et al., 2017; Hua et al., 2008; Kwon et al., 2011; Lagutina et al., 2011, 2013; Tecirlioglu et al., 2006; Yamochi et al., 2013).…”

Section: Achievements and Limitations Of Iscntmentioning

confidence: 99%

The perspective of the incompatible of nucleus and mitochondria in interspecies somatic cell nuclear transfer for endangered species

Mrowiec

Bugno‐Poniewierska

Młodawska

2020

Reprod Domestic Animals

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Taking into account the latest Red List of the International Union for Conservation of Nature in which 25% of all mammals are threatened with extinction, somatic cell nuclear transfer (SCNT) could be a beneficial tool and holds a lot of potential for aiding the conservation of endangered, exotic or even extinct animal species if somatic cells of such animals are available. In the case of shortage and sparse amount of wild animal oocytes, interspecies somatic cell nuclear transfer (iSCNT), where the recipient ooplasm and donor nucleus are derived from different species, is the alternative SCNT technique. The successful application of iSCNT, resulting in the production of live offspring, was confirmed in several combination of closely related species. When nucleus donor cells and recipient oocytes have been used in many other combinations, very often with a very distant taxonomical relation iSCNT resulted only in the very early stages of cloned embryo development. Problems encountered during iSCNT related to mitochondrial DNA (mtDNA)/genomic DNA incompatibility, mtDNA heteroplasmy, embryonic genome activation of the donor nucleus by the recipient oocyte and availability of suitable foster mothers for iSCNT embryos. Implementing assisted reproductive technologies, including iSCNT, to conservation programmes also raises concerns that the production of genetically identical populations might cause problems with inbreeding. The article aims at presenting achievements, limitations and perspectives of iSCNT in maintaining animal biodiversity.

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Potential role of intraspecific and interspecific cloning in the conservation of wild mammals

Cited by 20 publications

References 57 publications

Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking

Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking

New developments in the field of genomic technologies and their relevance to conservation management

The perspective of the incompatible of nucleus and mitochondria in interspecies somatic cell nuclear transfer for endangered species

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