Potential role of dietary chitosan nanoparticles against immunosuppression, inflammation, oxidative stress, and histopathological alterations induced by pendimethalin toxicity in Nile tilapia

Elbahnaswy, Samia; Elshopakey, Gehad E.; Ibrahim, Iman; Habotta, Ola A.

doi:10.1016/j.fsi.2021.09.015

Cited by 13 publications

(3 citation statements)

References 71 publications

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“…This helpful association of curcumin with the metabolism and nutrients contained in Nile tilapia's blood has been reported previously (Fazio 2019 [31,33,38,63,92]. [1,93].…”

Section: Discussionsupporting

confidence: 77%

Investigating the Potential of dietary additives, Spirulina platensis and Curcumin Nanoparticles in Nile Tilapia Fingerlings: A Comprehensive Analysis of Growth, Health, and Immune Function

Eissa,

Khattab,

Elbahnaswy

et al. 2023

Preprint

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A total of 180 Nile tilapia fingerlings (10 ± 0.5 g) were separated into three groups (n = 60), including a control group and two experimental groups supplemented with Spirulina platensis (SP; 5 g.kg-1) and curcumin nanoparticles (Cu-NPs; 30 mg.kg-1), respectively. After a 56-day feeding study, an evaluation of growth performance, hematological factors, antioxidant activity, biochemical and immunological characteristics, and histological modifications were conducted. The results revealed that the SP- and Cu-NPs supplements significantly improved growth indices, carcass features and biochemical features in both experimental groups compared to the control. Furthermore, the activities of digestive enzymes, comprising amylase, lipase, and protease, were significantly increased in the SP and Cu-NPs groups, unlike the control group. Additionally, supplementation with Cu-NPs and SP significantly enhanced the antioxidant state, as exhibited by increased glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity and decreased activity of malondialdehyde (MDA). The immune response was also significantly improved, with higher serum IgM and lysozyme activity in the SP and Cu-NPs treatments. Therefore, this study provides evidence that dietary supplementation with Cu-NPs and SP efficiently enhances the activity of digestive enzymes, antioxidant capacity, growth and performance, hepatoprotective capabilities, histopathology, and immune responses of Nile tilapia fingerlings.

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“…This helpful association of curcumin with the metabolism and nutrients contained in Nile tilapia's blood has been reported previously (Fazio 2019 [31,33,38,63,92]. [1,93].…”

Section: Discussionsupporting

confidence: 77%

Investigating the Potential of dietary additives, Spirulina platensis and Curcumin Nanoparticles in Nile Tilapia Fingerlings: A Comprehensive Analysis of Growth, Health, and Immune Function

Eissa,

Khattab,

Elbahnaswy

et al. 2023

Preprint

Self Cite

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“…Following the manufacturer’s instructions, 1 µg of total RNA was used to synthesize complementary DNA (cDNA) using a TOPscript™ RT DryMIX(dT18) cDNA Synthesis Kit (Enzynomics Co. Ltd., Daejeon, Republic of Korea), according to the manufacturer's protocol. The specific primers used to amplify the selected genes of Nile tilapia with stress marker genes, heat shock protein-70 ( HSP-70 ), proinflammatory genes, tumor necrosis factor-alpha ( TNF-α ), anti-inflammatory genes, Transforming Growth Factor-β ( TGF-β1 ), and Interleukin-10 ( IL-10 ), in addition to β-actin as a housekeeping gene, have been previously described [ 31 , 32 ]. Solg™ 2X Real-Time PCR Smart mix (Including SYBR® Green) was used in conjunction with the QuantStudio1™ Real-Time PCR System (Applied Biosystems™ Thermo Fisher Scientific, USA) to quantitatively analyze gene expression (SolGent Co., Ltd. Yuseong-gu, Daejeon, Korea).…”

Section: Methodsmentioning

confidence: 99%

Dietary Pediastrum boryanum microalgal extract improves growth, enhances immunity, and regulates immune-related genes in Nile tilapia

Al-Wakeel,

Elbahnaswy,

Risha

et al. 2024

BMC Vet Res

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Background Identifying alternative sustainable feed sources with high nutritional values is crucial for the future of environmentally and socially responsible aquaculture. In this regard, microalgae have been proven to have positive effects on fish health, which overwhelmed our interest in this study. Methods Pediastrum boryanum (P. boryanum) was incorporated into Nile tilapia feed at concentrations of 0, 0.75, and 1.5 mg/kg, as control, PbExt0.75, and PbExt1.5 groups to assess its effects on growth and biochemical indices, oxidant/antioxidant activities, immune and stress-related gene expression, and intestinal morphology. Results After 8 weeks, fish fed P. boryanum supplemented feed exhibited significant increases in final weight, length, condition factor, body weight gain, and specific growth rate, while the spleen-somatic index (SSI) and hepatosomatic index (HSI) showed no significant differences compared to the control group. Dietary P. boryanum supplementation also enhanced IgM levels and lysozyme activity, along with no marked effect on markers of liver function enzymes (alanine aminotransferase/ALT and aspartate aminotransferase/AST) or protein status (total protein and albumin). Furthermore, P. boryanum addition increased the activity of superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) enzymes, highlighting its antioxidant potential, whereas malondialdehyde (MDA) concentrations showed no significant differences among the groups. Gene expression analysis revealed that tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), and transforming growth factor-β1 (TGF-β1) expression notably increased in groups fed P. boryanum containing feed, while no significant difference was observed in hepatic Heat Shock Protein 70 (HSP70) mRNA expression. Histopathological examination revealed no adverse effects of P. boryanum supplementation on the liver, spleen, or intestinal tissues. Villous height and villous surface area were notably increased in the high P. boryanum supplementation group, suggesting improved intestinal integrity and nutrient absorption. Conclusion Dietary P. boryanum supplementation can potentially improve growth performance, immune response, antioxidant status, and intestinal health of Nile tilapia, making it a promising candidate for sustainable aquaculture.

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“…Synthetic or natural immunostimulants (such as, glucans, probiotics, trace elements, cytokines, hormones, and products derived from animals, algae, and herbs) can effectively stimulate fish growth, immunological response, and resistance to environmental stressors [16]. However, the administration of hormones, vitamins, and chemical products is frequently not recommended because they could cause adverse reactions in fish health and generate potentially hazardous residues for consumers [17][18][19]. Set against this, plant-based constituents might be successful vaccinations instead of common drugs and antibiotics since they provide physiologically active secondary metabolites that are easily accessible, cheap, and nontoxic [20,21].…”

Section: Introductionmentioning

confidence: 99%

The effects of dietary Spirulina platensis or curcumin nanoparticles on performance, body chemical composition, blood biochemical, digestive enzyme, antioxidant and immune activities of Oreochromis niloticus fingerlings

Eissa,

Khattab,

Elbahnaswy

et al. 2024

BMC Vet Res

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Context Recently, prioritize has been given to using natural phytogenic or nano compounds as growth promoters and immunostimulants in fish diets as an alternative to antibiotics. Aims The main propose of this trial was to determine the impact of supplementing diets with spirulina or curcumin nanoparticles on the performance and health indicators of Nile tilapia fingerlings. Methods In a 56-day feeding trial, 180 tilapia fingerlings were assigned into three main groups, as follows: 1st, control group, 2nd, Spirulina platensis (SP; 5 g kg-1 diet) and 3rd, curcumin nanoparticles (CUR-NPs; 30 mg kg-1 diet). Key results Incorporating tilapia diets with SP or CUR-NPs significantly improved performance, body chemical analysis, blood biochemical and hematological indices, digestive enzyme activities, and antioxidant and immunostimulant features compared to the control. Conclusion Fortified tilapia diets with CUR-NPs or SP efficiently boost the productivity and health of Nile tilapia fingerlings. Implications The research introduces new practical solutions for applying safe feed additives as alternatives to antibiotics in tilapia farming.

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Potential role of dietary chitosan nanoparticles against immunosuppression, inflammation, oxidative stress, and histopathological alterations induced by pendimethalin toxicity in Nile tilapia

Cited by 13 publications

References 71 publications

Investigating the Potential of dietary additives, Spirulina platensis and Curcumin Nanoparticles in Nile Tilapia Fingerlings: A Comprehensive Analysis of Growth, Health, and Immune Function

Investigating the Potential of dietary additives, Spirulina platensis and Curcumin Nanoparticles in Nile Tilapia Fingerlings: A Comprehensive Analysis of Growth, Health, and Immune Function

Dietary Pediastrum boryanum microalgal extract improves growth, enhances immunity, and regulates immune-related genes in Nile tilapia

The effects of dietary Spirulina platensis or curcumin nanoparticles on performance, body chemical composition, blood biochemical, digestive enzyme, antioxidant and immune activities of Oreochromis niloticus fingerlings

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