Potential regulatory role of circular RNA in idiopathic pulmonary fibrosis

Li, Rongrong; Wang, Youlei; Song, Xiaodong; Sun, Wenjing; Zhang, Jinjin; Liu, Yuxia; Li, Hongbo; Meng, Chao; Zhang, Jie; Zheng, Qingyin; Lv, Changjun

doi:10.3892/ijmm.2018.3892

Cited by 44 publications

(58 citation statements)

References 51 publications

(51 reference statements)

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“…By contrast, lncRNAs such as lncCHRF, n341773 and H19 contribute to lung fibrosis by acting as miRNA sponges . Based on our previous studies, competing endogenous RNAs are present not only in lncRNAs but also in circRNAs . In this study, we showed the existence of competing endogenous RNAs under drug action.…”

Section: Discussionmentioning

confidence: 61%

“…Each bar represents the mean ± SD, n = 6, *P < .05, **P < .01 circRNAs. 19,43 In this study, we showed the existence of competing 47 Lee et al analysed the transcriptome complexity through RNA sequencing in normal and failing murine hearts. 48 However, few studies have identified the profiles of differentially expressed circRNAs under drug action using RNA sequencing in progressive lung fibrosis.…”

Section: Discussionmentioning

confidence: 63%

“…32,33 A recent study from our laboratory based on a circRNA microarray analysis identified 67 significantly dysregulated circRNAs in IPF patients, indicating the fundamental roles of circRNAs in pathological processes of lung fibrosis. 19 In this study, 145 differentially expressed circRNAs were identified under astilbin treatment using RNA sequencing.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study, we found that ncRNAs play key roles in fibrosis and can be used as therapeutic targets for pulmonary fibrosis using microarray technology . However, microarray technology lacks sensitivity for low‐abundance RNA and may miss RNAs related to disease risk and progression.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, we found that ncRNAs play key roles in fibrosis and can be used as therapeutic targets for pulmonary fibrosis using microarray technology. [17][18][19] However, microarray technology lacks sensitivity for low-abundance RNA and may miss RNAs related to disease risk and progression. Moreover, compared with miRNA and lncRNA, circRNA is a novel class of RNA transcript and a new clinical diagnostic and prognostic biomarker.…”

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confidence: 99%

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Regulatory network of two circRNAs and an miRNA with their targeted genes under astilbin treatment in pulmonary fibrosis

Zhang

Liu

et al. 2019

J Cellular Molecular Medi

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Circular RNAs (circRNAs) are becoming new therapeutic drug targets. However, their profiles under astilbin treatment have not been reported yet. In this study, we analysed the global reprogramming of circRNA transcriptome and a regulatory network of circRNAs with their targeted genes under astilbin treatment in pulmonary fibrosis. A total of 145 circRNAs were differentially expressed in the astilbin‐treated group compared with the bleomycin‐treated group using RNA sequencing. In the bleomycin‐ and astilbin‐treated groups, 29 coexpressed circRNAs were found. The maximum number of circRNAs was distributed on chromosome two, and their length varieties were mainly within 1000 bp. Four differentially expressed circRNAs (circRNA‐662, 949, 394 and 986) were tested to validate the RNA sequencing data, and their targeted microRNAs and genes were analysed by qRT‐PCR, Western blot, Pearson correlation coefficient, a dual‐luciferase reporter system and anti‐AGO2 RNA immunoprecipitation. The results showed that circRNA‐662 and 949 can act as “miR‐29b sponges” targeting Gli2 and STAT3 to exert their functions. Our work suggests that the transcriptome complexity at the circRNA level under astilbin treatment. These circRNAs may be potential molecular targets for drug action.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Regulatory network of two circRNAs and an miRNA with their targeted genes under astilbin treatment in pulmonary fibrosis

Zhang

Liu

et al. 2019

J Cellular Molecular Medi

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Exosome‐transmitted circ_MMP2 promotes hepatocellular carcinoma metastasis by upregulating MMP2

et al. 2020

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Exosomes released by tumor cells have been recently identified as important determinants of tumor progression. They often carry circular RNAs that are differentially expressed in tumors and may regulate tumorigenesis and metastasis. Here, we showed that supernatant of 97H hepatocellular carcinoma (HCC) cell line could promote metastasis in L02 human liver cells and HCC cell lines. Moreover, we determined that circ_MMP2 (has_-circ_0039411) could be delivered by 97H-or LM3 cell-derived exosomes to L02 and HepG2 cell cultures. High expression of circ_MMP2 led to the upregulation of its host gene matrix metallopeptidase 2 (MMP2) via the sponging of miR-136-5p. Rescue assays demonstrated that miR-136-5p and MMP2 were two essential participants in HCC metastasis. Finally, high level of circ_MMP2 or MMP2, as well as low level of miR-136-5p, was correlated with low overall survival of HCC patients. Our study highlights a novel molecular pathway in HCC cell-derived exosomes.

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Retracted: Circular RNA ANKRD36 attends to lipopolysaccharide‐aroused MRC‐5 cell injury via regulating microRNA‐31‐3p

2019

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Background Some circular RNAs (circRNAs) are reported to attend to the pathogenesis of pneumonia. This study tested the impact of circRNA ankyrin repeat domain 36 (circANKRD36) on human embryonic lung fibroblast MRC‐5 cell injury irritated by lipopolysaccharide (LPS). Methods After LPS irritation, viability, apoptosis, ROS, protein, and cytokines, along with circANKRD36 were tested by CCK‐8, Annexin V‐FITC, DCFH‐DA, and ELISA or Western blot. si‐circANKRD36 and microRNA‐31‐3p/5p (miR‐31‐3p/5p) inhibitor were applied to silence circANKRD36 and miR‐31‐3p/5p. miR‐31‐3p/5p mimic was utilized to upregulate miR‐31‐3p/5p. RT‐qPCR was used to detect miRNAs. The relationship between miRNAs and MyD88 or IL‐34 was analyzed by luciferase activity reporter assay. Results LPS aroused a decrease in viability, increases in apoptosis, ROS, and IL‐6, IL‐8, and TNF‐α, along with circANKRD36, and activation of NF‐κB pathway. Silencing circANKRD36 weakened the above‐mentioned influences of LPS. Moreover, silencing circANKRD36 hoisted miR‐31‐3p expression. Silencing miR‐31‐3p mitigated the impacts of circANKRD36 silence on LPS‐irritated MRC‐5 cells. Besides, MyD88 was a downstream target of miR‐31‐3p, and 3′UTR of IL‐34 mRNA was targeted by miR‐31‐5p. LPS induced the accumulation of MyD88. Silencing MyD88 was constructive to maintain cell viability, retard apoptosis and inhibit adverse oxidation and inflammation. Conclusion This research verified that silencing circANKRD36 could weaken LPS‐irritated MRC‐5 cell injury via regulating miR‐31/MyD88‐mediated repression of NF‐κB pathway.

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Potential regulatory role of circular RNA in idiopathic pulmonary fibrosis

Cited by 44 publications

References 51 publications

Regulatory network of two circRNAs and an miRNA with their targeted genes under astilbin treatment in pulmonary fibrosis

Regulatory network of two circRNAs and an miRNA with their targeted genes under astilbin treatment in pulmonary fibrosis

Exosome‐transmitted circ_MMP2 promotes hepatocellular carcinoma metastasis by upregulating MMP2

Retracted: Circular RNA ANKRD36 attends to lipopolysaccharide‐aroused MRC‐5 cell injury via regulating microRNA‐31‐3p

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