Potential protective effect of Nigella sativa crude oils towards fatty liver in rats

Al‐Okbi, Sahar Y.; Mohamed, Doha A.; Hamed, Thanaa E.; Edris, Amr E.

doi:10.1002/ejlt.201200256

Cited by 28 publications

(20 citation statements)

References 78 publications

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“…Nigella sativa oil showed previously to produce a hepatoprotective effect in rats (Al-Suhaimi, 2012) which was confirmed by the work of Al-Okbi et al (2013), who reported its protective effect against steatohepatitis. This effect was ascribed to the volatile oil fraction, particularly thymoquinone and p-cymene and to a lesser extent to the fatty acid profile of Nigella crude oil.…”

Section: Discussionmentioning

confidence: 65%

“…Rats with MHRS showed a significant elevation in all liver fat with dyslipidemia which may be due to feeding high fructose as described by Kawasaki et al (2009) andAl-Okbi et al (2013). Nigella sativa oil showed previously to produce a hepatoprotective effect in rats (Al-Suhaimi, 2012) which was confirmed by the work of Al-Okbi et al (2013), who reported its protective effect against steatohepatitis.…”

Section: Discussionmentioning

confidence: 69%

“…Oil soluble vitamins were given orally in a dose of 0.1 mL/rat/week. Two types of diets were prepared; a balanced and a high fructose diet as described by Kawasaki et al (2009) and Al-Okbi et al (2013).…”

Section: Dietsmentioning

confidence: 99%

“…The rats of groups 3, 4 and 5 were fed a high fructose diet and given a daily oral dose of 265mg·kg −1 rat body weight of N. sativa crude oil, a mixture of N. sativa crude oil and fish oil (1:1) and a mixture of N. sativa crude oil and flaxseed oil (1:1), respectively by stomach tube. The dose of 265 mg·kg −1 was used because N. sativa crude oil was reported previously to improve fatty liver at this dose level (Al-Okbi et al, 2013). On the 34 th day all rats except the normal healthy control (group: 1) were given 1.1 g·kg −1 body weight of galactosamine hydrochloride via intraperitoneal injection as a 200 mg·mL −1 solution in saline to induce hepatorenal syndrome (Saracyn et al, 2012).…”

Section: Biological Experimentsmentioning

confidence: 99%

“…So, in the present research galactosamine hydrochloride was used to induce HRS in Sprague dawley rats according to Saracyn et al (2012). In our previous work, (Al-Okbi et al, 2013) it was revealed that the crude oil of Nigella sativa had a promising protection from steatohepatitis, which is a fatty liver with inflammation in rats.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the therapeutic effect of Nigella sativa crude oil and its blend with omega-3 fatty acid-rich oils in a modified hepatorenal syndrome model in rats

et al. 2015

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SUMMARY:In the present study, the hepato and reno-protective effect of Nigella sativa crude oil and its binary blend with omega-3 fatty acid-rich oils (fish and flaxseed oils) was studied in a modified hepatorenal syndrome model (MHRS) in rats. MHRS was induced through feeding a high fructose diet followed by an intraperitoneal injection of galactosamine hydrochloride. Nigella oil and its different blends were given as a daily oral dose to MHRS rats. Two control groups of MHRS and normal healthy rats were run. Different biochemical and nutritional parameters were assessed. The induction of MHRS produced liver and kidney dysfunction, and elevated oxidative stress, an inflammatory biomarker, endothelin 1, and plasma cholesterol. Reduced plasma high density lipoprotein cholesterol, albumin and Ca and elevated urinary N-acetyl-β-D-Glucosaminidase and liver fats were noticed. The administration of Nigella crude oil that originally had 0.2% total omega-3 fatty acids or its blend with fish oil (17.9% omega-3) or flaxseed oil (42.1% omega-3) significantly improved all biochemical parameters of MHRS. There was no significant difference in the biochemical parameters among the different oil treated groups regardless of the omega-3 fatty acid content. This may point out to the potential profound effect of the volatile oil fraction of Nigella crude oil which may compensates for its low omega-3 content. KEYWORDS: Hepatorenal syndrome; Nigella crude oil; Oil blends; Omega-3 fatty acidsRESUMEN: Evaluación del efecto terapéutico del aceite crudo de Nigella sativa y su mezcla con aceites ricos en ácidos grasos omega-3 en un modelo de síndrome hepatorenal modificado en ratas. En el presente estudio, el efecto hepato-y reno-protector de aceites crudos de Nigella sativa y su mezcla binaria con aceites ricos en ácidos grasos omega-3 (pescado y aceites de linaza) fue estudiado en un modelo modificado de síndrome hepatorenal (MHRS) en ratas. MHRS fue inducido a través de la alimentación de una dieta alta en fructosa seguido de la inyección intraperitoneal de clorhidrato de galactosamina. Diferentes aceites fueron suministrados como dosis oral diaria a ratas con MHRS. Se realizaron dos grupos de control de MHRS y ratas sanas normales. Se evaluaron diferentes parámetros bioquímicos y nutricionales. La inducción de la MHRS produce disfunción hepática y renal, elevado estrés oxidativo y biomarcadores inflamatorios, endotelina 1, y colesterol en plasma. Se observó una reducción del colesterol plasmatico de lipoproteínas de alta densidad, albúmina y Ca, elevación en orina de N-acetil-β-D-glucosaminidasa y de la grasa del hígado. La administración de aceite crudo de Nigella que tiene un 0,2% de ácidos grasos omega-3 totales o su mezcla con aceite de pescado (17,9% de omega-3) o aceite de linaza (42,1% de omega-3) mejoró significativamente todos los parámetros bioquímicos de MHRS. No hubo diferencia significativa en los parámetros bioquímicos entre los diferentes grupos tratados con los aceites ensayados independientemente del contenido de áci...

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Section: Discussionmentioning

confidence: 65%