Potential probiotic attributes of a new strain of Bacillus coagulans CGMCC 9951 isolated from healthy piglet feces

Abstract

A new strain of Bacillus coagulans CGMCC 9551, which has a broad range of antibacterial activities against six main pathogenic bacteria including Escherichia coli O8, Staphylococcus aureus, Salmonella enterica subsp. enterica serovar enteritidis, Streptococcus suis, Listeria monocytogenes and Pasteurella multocida, was isolated from healthy piglet feces. In adhesion assay, the isolate exhibited a stronger adhesion to pig intestinal mucus than that of B. subtilis JT143 and L. acidophilus LY24 respectively isolated from BioPlus(®)2B and FloraFIT(®) Probiotics (P < 0.05). The adhesion activity reached 44.5 ± 3.2, 48.9 ± 2.6, 42.6 ± 3.3 and 37.6 ± 2.4% to jejunum, ileum, transverse colon and sigmoid colon, separately. The survival rate of B. coagulans CGMCC 9551 was reduced by only 20% at 4 h exposure under 0.9% w/v bile salt. The strain was fully resistant to pH 2 for 2 h with 90.1 ± 3.5% survival and susceptible to 15 antibiotics commonly used in veterinary medicine. Additionally, the bacteria showed amylase, protease and cellulase activities. The safety assessment demonstrated the lack of toxicity potential in B. coagulans CGMCC 9551 by ligated rabbit ileal loop assay, acute and subchronic toxicity test. These results implied that that the new strain of B. coagulans CGMCC 9951 isolated from healthy piglet feces has promising probiotic characteristics and offers desirable opportunities for its successful commercialization as one excellent candidate probiotic.

Application of Bacillus coagulans in Animal Husbandry and Its Underlying Mechanisms

“…Such a beneficial effect is probably owed to the promoted secretion of endogenous enzymes which improves the nutrient digestibility [35]. Moreover, B. coagulans also produces some exogenous enzymes and unknown growth-promoting factors which could increase the intestinal peristalsis and the feed digestibility [44,45]. B. coagulans produces α-galactosidase which strongly resists hydrolysis by protease [46].…”

Solid-state fermentation technology and innovation for the production of agricultural and animal feed bioproducts

“…However, the Bacillus genus has been gaining space in the probiotics market, especially due to its spore formation capacity and, therefore, the formation of a product resistant to adverse conditions [157,158]. Bacillus strains with a probiotic character mainly include the B. subtilis, B. licheniformis and B. amyloliquefaciens species [159][160][161], and also some species such as B. coagulans [162] and B. indicus [163].…”

Complete genome sequence of Bacillus velezensis 157 isolated from Eucommia ulmoides with pathogenic bacteria inhibiting and lignocellulolytic enzymes production by SSF

“…have the potential of secreting abundant extracellular enzyme. Bacillus coagulans CGMCC 9951 isolated from healthy piglet feces showed the capacity to hydrolyze protein, starch and cellulose (Gu et al 2015). Bacillus coagulans CMB3 also showed the ability to produce more extracellular enzyme, including α-amylase, protease, pectinase, chitinase and lecithinase (Mishra et al 2009).…”