Potential of the endogenous and artificially inserted CRISPR-Cas system for controlling virulence and antimicrobial resistance of food pathogens

Polyphenols and CRISPR as Quorum Quenching Agents in Antibiotic-Resistant Foodborne Human Pathogens (Salmonella Typhimurium, Campylobacter jejuni and Escherichia coli 0157:H7)

“…Verified the variation in the reduction of mobility that exists in flagellum phenotypes [123] Costigan et al ( 2022) were able to validate the repression of the astA gen which encodes for arilsulfatase using the CRISPR interference (CRISPRi) in Campylobacter jejuni [122]. The same tool was applied to a deleted M1Cam strain showing that the Cas9 endogenous system did not affect the CRISPRi system.…”

“…Cas9 from C. jejuni is toxic to human cells, so the C. jejuni Cas9 gene, transcript and protein are a perfect target to control or regulate the infectious processes caused by this bacterium. For Campylobacter, as well as for other bacteria, once confirmed that it carries a CRISPR-Cas9 type system, it is recommended that, in addition to using other combined CRISPR systems of the type Cas3, dCas9, Cas13a, dCas13 or others, all this is used for the system to be effective (Figure 2) [123]. Like S. typhi, C. jejuni expresses genes from the LuxS family [119,120]; therefore, it is also considered a good target to develop CRISPR-type regulation tools.…”