Potential of Streptomyces sp. in preventing the in vitro growth of Colletotrichum acutatum, the causative agent of infection in Capsicum annum L.

Putri, Rochmalia Juniarti; Kawuri, Retno; Darmadi, Anak Agung Ketut; Narayani, Inna

doi:10.24843/jbiounud.2021.v25.i02.p12

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…isolates. and Nocardia (Putri et al, 2021). Acid-resistant bacteria in this staining method will produce a pink color, while non-AFB bacteria (acid-resistant bacteria) will display a blue or green color (Misnarliah et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Exploration Streptomyces sp. on the Soil of Several Vegetations in the Forest Zone Area of Alas Purwo Banyuwangi National Park and Tests of its Inhibitory on Escherichia coli O157:H7

Insani,

R. Kawuri,

Muksin

et al. 2023

EEC

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Problems related to antibiotic resistance are getting higher every time. Therefore, studies related to the exploration of new sources of antibiotics are very important to do. One source of antibiotics that has the potential to be utilized is derived from Streptomyces sp. This bacteria belongs to the Gram-positive bacteria of the Streptomycetaceae family which has the ability to produce enzyme and antibiotic is often found in natural soils. This study aims to explore the bacteria Streptomyces sp. in grassland vegetation and tropical rain forests in the jungle zone in the Alas Purwo National Park Area East Java Indonesia and tested its ability to inhibit the growth of Escherichia coli O157:H7. Sampling in this study was carried out in two different vegetation areas of the Alas Purwo National Park. The first vegetation is Sadengan grassland and the second is forest in the jungle zone. Soil samples were taken for each type of vegetation at five points. To isolate Streptomyces sp. used pour plate method with selective media YEMA (Yeast Extract Malt Agar). Bacterial isolates suspected as Streptomyces sp. and different from one another are separated to be re-isolated and identified macroscopically and microscopically Seung and Good fellow (2002). Twenty Streptomyces sp. isolates were successfully isolated from 2 vegetations. The highest inhibition was Streptomyces sp. O (2.725 cm) against Escherchia coli O157:H7, while the one with the lowest inhibition was Streptomyces sp .G (0.542 cm). In the future, after carrying out molecular tests, Streptomyces sp.O can be used as an alternative new antibiotics to combat pathogenic bacteria.

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Section: Discussionmentioning

confidence: 99%

Exploration Streptomyces sp. on the Soil of Several Vegetations in the Forest Zone Area of Alas Purwo Banyuwangi National Park and Tests of its Inhibitory on Escherichia coli O157:H7

Insani,

R. Kawuri,

Muksin

et al. 2023

EEC

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“…Isolates that have grown will be identified on the macroscopic and microscopic shape of Streptomyces spp. (Putri et al, 2021).…”

Section: Streptomyces Spp Isolation and Identificationmentioning

confidence: 99%

Study of Streptomyces spp. to control purple blotch disease caused by Alternaria porri in shallot plant

Risdiyanti¹,

Rahmadhini²,

Suryaminarsih³

et al. 2023

CROPSAVER

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Alternaria porri is a fungal pathogen that causes purple blotch on shallots, this fungus can cause crop yield loss of 3 – 57%. A. porri was obtained from the Sumber Brantas onion farm and then isolated by planting infected tissue. Streptomyces spp. is a filamentous bacterium that is abundant in the soil and can be used as a biological agent, decomposer and plant growth promote. Streptomyces spp. was obtained one isolate from shallot land location in Pare, Kediri, East Java (BMP: Bawang Merah Pare) and three isolates from Sidera, Palu, Central Sulawesi (BMS: Bawang Merah Sidera) (BMS1, BMS2, BMS3). The purpose of this study was to determine the efficacy of Streptomyces spp. to control A porri consist of in vitro and In vivo antagonist test. Antagonist test was carried out by dual culture method and the Streptomyces spp. which can inhibit the development of A porri would be used antagonist test in vivo. The study was designed using a Factorial Complete Randomized Design with two factors, candidate isolates of biological control agents and concentrations of Streptomyces spp. be diluted into 5%, 10%, and 15%, it will applied on shallot plant Bauji variety. The results of the In vitro test shown the highest inhibition zone from BMP 17.75% while BMS1 13.75%, BMS2 8,75%, and 8.50%. in vivo test shown lowest disease severity value in BMP 15% concentration was 1.13% while diseases severity of BMS1 was 1.27%, and BMS2 was 1.80%. Therefore, Streptomyces spp. has potency as an alternative pesticide for sustainable agriculture.

show abstract

Potential of Streptomyces sp. in preventing the in vitro growth of Colletotrichum acutatum, the causative agent of infection in Capsicum annum L.

Cited by 2 publications

References 4 publications

Exploration Streptomyces sp. on the Soil of Several Vegetations in the Forest Zone Area of Alas Purwo Banyuwangi National Park and Tests of its Inhibitory on Escherichia coli O157:H7

Exploration Streptomyces sp. on the Soil of Several Vegetations in the Forest Zone Area of Alas Purwo Banyuwangi National Park and Tests of its Inhibitory on Escherichia coli O157:H7

Study of Streptomyces spp. to control purple blotch disease caused by Alternaria porri in shallot plant

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