Potential of Serratia plymuthica IV-11-34 strain for biodegradation of polylactide and poly(ethylene terephthalate)

Dąbrowska, Grażyna B.; Tylman-Mojżeszek, Wioleta; Mierek‐Adamska, Agnieszka; Richert, Agnieszka; Hrynkiewicz, Katarzyna

doi:10.1016/j.ijbiomac.2021.10.063

Cited by 7 publications

(4 citation statements)

References 63 publications

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“…The expression levels of the following genes were analyzed: chiA , budA , hcnC , iucA , entB , pchB , katG , sodB , and mtlR . The genes gyrA and gyrB were used as an internal controls, as commonly used standards in the study of gene expression of bacteria belonging to various genera, including those of Serratia [ 100 , 101 , 102 ]. The expression of internal genes was stable (CT = 15) across the treatments.…”

Section: Methodsmentioning

confidence: 99%

Genetic Determinants of Antagonistic Interactions and the Response of New Endophytic Strain Serratia quinivorans KP32 to Fungal Phytopathogens

Chlebek

Grebtsova

Piński

et al. 2022

IJMS

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Fungal phytopathogens are challenging to control due to their penetration into plant tissues. Therefore, plant-colonizing bacteria could serve as an excellent weapon in fighting fungal infections. In this study, we aim to determine the biocontrol potential of the new endophytic strain Serratia quinivorans KP32, isolated from the roots of Petroselinum crispum L.; identify the related mechanisms; and understand the basis of its antagonistic interaction with taxonomically diverse fungi at the molecular level. The KP32 strain presented biological activity against Rhizoctonia solani, Colletotrichum dematium, Fusarium avenaceum, and Sclerotinia sclerotiorum, and its ability to inhibit the growth of the phytopathogens was found to be mediated by a broad spectrum of biocontrol features, such as the production of a number of lytic enzymes (amylases, chitinases, and proteases), siderophores, volatile organic and inorganic compounds, salicylic acid, and N-acyl-homoserine lactones. The higher expression of chitinase (chiA) and genes involved in the biosynthesis of hydrogen cyanide (hcnC), enterobactin (entB), and acetoin (budA) in bacteria exposed to fungal filtrates confirmed that these factors could act in combination, leading to a synergistic inhibitory effect of the strain against phytopathogens. We also confirm the active movement, self-aggregation, exopolysaccharide production, and biofilm formation abilities of the KP32 strain, which are essential for effective plant colonization. Its biological activity and colonization potential indicate that KP32 holds tremendous potential for use as an active biopesticide and plant growth promoter.

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Section: Methodsmentioning

confidence: 99%

Genetic Determinants of Antagonistic Interactions and the Response of New Endophytic Strain Serratia quinivorans KP32 to Fungal Phytopathogens

Chlebek

Grebtsova

Piński

et al. 2022

IJMS

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“…PET degradation methods mainly include physical methods [8][9][10][11][12][13] and chemical methods. [14][15][16][17][18][19][20] Physical methods are used to recycle waste polyester bottles by means of crushing and melting without changing the chemical structure, and only low value-added products can be obtained in this way due to reduced performance. Chemical methods, on the other hand, mainly rely on hydrolysis and alcoholysis.…”

Section: Introductionmentioning

confidence: 99%

Preparation of functionalised heteropolyacid ionic liquids and their application in catalytic degradation of bottle-grade polyester

Liu,

Liao

et al. 2023

New J. Chem.

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“…Bacterial species under the genus Serratia, belonging to the family Enterobacteriaceae, has been identified for its numerous applications in biodegradation (Majumdar et al, 2020 ; An et al, 2021 ; Dabrowska et al, 2021 ). Serratia proteamaculans , a gram-negative, non-pigmented strain under this genus, was first reported to cause a leaf spot disease and is the only identified phytopathogen under the genus Serratia (Paine and Standsfield, 1919 ; Mahlen, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of a New Serratia proteamaculans Strain That Naturally Produces Significant Amount of Extracellular Laccase

et al. 2022

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Natural biodegradation processes hold promises for the conversion of agro-industrial lignocellulosic biomaterials into biofuels and fine chemicals through lignin-degrading enzymes. The high cost and low stability of these enzymes remain a significant challenge to economic lignocellulosic biomass conversion. Wood-degrading microorganisms are a great source for novel enzyme discoveries. In this study, the decomposed wood samples were screened, and a promising γ-proteobacterial strain that naturally secreted a significant amount of laccase enzyme was isolated and identified as Serratia proteamaculans AORB19 based on its phenotypic and genotypic characteristics. The laccase activities in culture medium of strain AORB19 were confirmed both qualitatively and quantitatively. Significant cultural parameters for laccase production under submerged conditions were identified following a one-factor-at-a-time (OFAT) methodology: temperature 30°C, pH 9, yeast extract (2 g/l), Li+, Cu2+, Ca2+, and Mn2+ (0.5 mM), and acetone (5%). Under the selected conditions, a 6-fold increase (73.3 U/L) in laccase production was achieved when compared with the initial culturing conditions (12.18 U/L). Furthermore, laccase production was enhanced under alkaline and mesophilic growth conditions in the presence of metal ions and organic solvents. The results of the study suggest the promising potential of the identified strain and its enzymes in the valorization of lignocellulosic wastes. Further optimization of culturing conditions to enhance the AORB19 strain laccase secretion, identification and characterization of the purified enzyme, and heterologous expression of the specific enzyme may lead to practical industrial and environmental applications.

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Potential of Serratia plymuthica IV-11-34 strain for biodegradation of polylactide and poly(ethylene terephthalate)

Cited by 7 publications

References 63 publications

Genetic Determinants of Antagonistic Interactions and the Response of New Endophytic Strain Serratia quinivorans KP32 to Fungal Phytopathogens

Genetic Determinants of Antagonistic Interactions and the Response of New Endophytic Strain Serratia quinivorans KP32 to Fungal Phytopathogens

Preparation of functionalised heteropolyacid ionic liquids and their application in catalytic degradation of bottle-grade polyester

Identification and Characterization of a New Serratia proteamaculans Strain That Naturally Produces Significant Amount of Extracellular Laccase

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