Potential of rare actinomycetes in the production of metabolites against multiple oxidant agents

Abstract

Context: Actinobacteria are a precious source of novel bioactive metabolites with potential pharmaceutical applications.Objectives: Representatives of 11 genera of rare Actinobacteria were selected for the evaluation of antioxidant activity.Material and methods: Fermentation broths of the Actinobacteria were extracted and dosage of 10 to 2000 µg/mL were applied for in vitro antioxidant-related bioassays. Cytotoxicity was assessed at the concentration of 2.5–20 µg/mL.Results: In the DPPH scavenging activity, 15 out of 52 extracts showed 17.0–26.8% activity in quantitative evaluation. Metabolites of five prominent antioxidant producing strains protected the DNA (pUC19) against UV-induced photolyzed H2O2-oxidative degradation. The potent antioxidant extracts inhibited two oxidative enzymes of xanthine oxidase in the range of 17.5–45.2% (three extracts had IC50 less than allopurinol) and lipoxygenase in the range of 36–55% (all five extracts had IC50 values less than daidzein). All these extracts could also protect eythrocytes from iron-induced hemolysis with ED50 values in a range of 0.014–1.25 mg/mL. Growth restoration of the yeast cells lacking the sod1 gene was observed by the antioxidant metabolite of Saccharothrix ecbatanensis UTMC 537 at the concentration of 1 mg/mL.Conclusions: The presence of nonidentical metabolites might be responsible for antioxidant and enzyme inhibitory activities of S. ecbatanensis, newly described actinobacterium in family Pseudonocardiaceae. The scavenging of the free electrons, protection of DNA and model yeast cells against oxidative stress, in addition to the inhibition of the oxidating enzymes are the main mechanisms of the antioxidant effect of the introduced resource in this study.

Potentially pathogenic bacteria isolated from diverse habitats in Spitsbergen, Svalbard

“…Actinobacteria species are generally regarded as an abundant source of metabolites such as antibiotics, antioxidants, and hemolytic as well as anti-hemolytic compounds (Goodfellow et al 2009;Suthindhiran and Kannabiran 2009;Pang et al 2016). We observed members of the genera Micromonospora, Streptomyces, and Oerskovia (isolates S3, S10, N18, N28, and N41) that exhibited hemolytic activity, supporting previous reports of species in these genera that might be considered opportunistic pathogens (McNeil and Brown 1994;Pang et al 2016;Mohammadipanah and Momenilandi 2018;Takahashi and Nakashima 2018).…”

Salinity Stress Enhances the Antioxidant Capacity of Bacillus and Planococcus Species Isolated From Saline Lake Environment

“…The study of natural sources of antioxidants revealed that numerous antioxidant compounds have been obtained from plants, mainly fruits, vegetables, nuts, and whole grains, as well as seafood, meat, and poultry (Shahidi and Zhong, 2010;Mohammadipanah and Momenilandi, 2018). Higher plants are considered the richest source of antioxidants and have been studied extensively (Kähkönen et al, 1999;Krishnaiah et al, 2011).…”

Introduction of marine-derivedStreptomycessp. UTMC 1334 as a source of pyrrole derivatives with anti-acetylcholinesterase activity

“…The anti‐oxidant activity of varying concentrations of the most efficient extract (10, 25, 50, 100 and 200 μ g ml −1 ) was measured on the basis of the scavenging activity of the stable 1,1‐diphenyl 2‐picrylhyorazyl (DPPH) (Sigma‐Aldrich)‐free radicals as described before (Brand‐Williams et al . ; Mohammadipanah and Momenilandi ). The sample solution (10 μ l) was mixed with 190 μ l DPPH (0·1 mmol l −1 ).…”