Potential of Probiotic Lactobacillus plantarum 2621 for the Management of Infertility

Bhandari, Praveen; Rishi, Praveen; Prabha, Vijay

doi:10.9734/bmrj/2014/12129

Cited by 4 publications

(5 citation statements)

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“…L. plantarum 2621 was able to colonize the mouse vagina efficiently. This showed association between the colonization observed in vivo with our in vitro studies of Lactobacillus attachment to the vaginal epithelium 10 . Other investigators 20 21 have used a large number of doses to ensure the colonization of Lactobacillus .…”

Section: Discussionsupporting

confidence: 70%

“…Carefully designed trials using well characterized probiotic strains and treatment regimens are required to evaluate the effect of probiotics on fertility outcome. L. plantarum 2621 has been shown to display in vitro properties relevant to colonization as assessed by adherence to vaginal epithelial cells 10 . The present study was undertaken to determine the capability of L. plantarum 2621 to overcome vaginal colonization and infertility induced by spermagglutinating E. coli , in a mouse model.…”

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confidence: 99%

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Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model

Bhandari¹,

Prabha²

2015

Indian J Med Res

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Background & objectives:Urogenital infections of bacterial origin have a high incidence among the female population at reproductive age, affecting the fertility. Strains of Escherichia coli can colonize the vagina and replace natural microflora. Lactobacillus the predominant vaginal microorganism in healthy women, maintains the acidic vaginal pH which inhibits pathogenic microorganisms. Studies on Lactobacillus have shown that these can inhibit E. coli growth and vaginal colonization. An alternative therapeutic approach to antimicrobial therapy is to re-establish Lactobacillus in this microbiome through probiotic administration to resurge fertility. Therefore, the aim of the present study was to determine the capability of L. plantarum 2621 strain with probiotic properties, to prevent the vaginal colonization of E. coli causing agglutination of sperms and to evaluate its profertility effect in a murine model.Methods:Screened mice were divided into five groups i.e. control group, E. coli group, Lactobacillus group, prophylactic and therapeutic groups. The control group was infused with 20 µl PBS, E.coli group was administered with 106 cfu/20 µl E. coli, and probiotic group was administered with Lactobacillus (108 cfu/20 µl) for 10 consecutive days. In prophylactic group, the vagina was colonized with 10 consecutive doses of Lactobacillus (108 cfu/20 µl). After 24 h, it was followed by 10 day intravaginal infection with E. coli (106 cfu/20 µl) whereas for the therapeutic group vagina was colonized with (106 cfu/20 µl) E. coli for 10 consecutive days, followed by 10 day intravaginal administration with Lactobacillus after 24 h.Results:Upon mating and completion of gestation period, control, probiotic and the therapeutic groups had litters in contrast to the prophylactic group and the group administered with E. coli.Interpretation & conclusions:Results indicated that Lactobacillus intermitted colonization of pathogenic strains that resulted in reinforcement of natural microflora and resurge fertility.

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Section: Discussionsupporting

confidence: 70%

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confidence: 99%

Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model

Bhandari¹,

Prabha²

2015

Indian J Med Res

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“…As beneficial reproductive effects of lactobacilli are more commonly reported than Bifidobacterium , most studies focus on strains of lactobacilli. The intrauterine administration of L. buchneri was associated with improved reproductive performance in cows and reduced inflammatory cytokines ( 244 ), while vaginal inoculation with L. plantarum restored fertility in mice that had previously been inoculated with a sperm agglutinating strain of E. coli ( 245 , 246 ). Additionally, vaginal treatment with L. plantarum was able to negate infertility caused by the intravaginal administration of LPS, indicating that probiotics can ameliorate inflammation-associated infertility, at least in a mouse model ( 247 ).…”

Section: Diagnostics and Therapeuticsmentioning

confidence: 99%

The female reproductive tract microbiotas, inflammation, and gynecological conditions

Gholiof

Luca

Wessels

2022

Front. Reprod. Health

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The intricate interactions between the host cells, bacteria, and immune components that reside in the female reproductive tract (FRT) are essential in maintaining reproductive tract homeostasis. Much of our current knowledge surrounding the FRT microbiota relates to the vaginal microbiota, where ‘health’ has long been associated with low bacterial diversity and Lactobacillus dominance. This concept has recently been challenged as women can have a diverse vaginal microbial composition in the absence of symptomatic disease. The structures of the upper FRT (the endocervix, uterus, Fallopian tubes, and ovaries) have distinct, lower biomass microbiotas than the vagina; however, the existence of permanent microbiotas at these sites is disputed. During homeostasis, a balance exists between the FRT bacteria and the immune system that maintains immune quiescence. Alterations in the bacteria, immune system, or local environment may result in perturbances to the FRT microbiota, defined as dysbiosis. The inflammatory signature of a perturbed or “dysbiotic” FRT microbiota is characterized by elevated concentrations of pro-inflammatory cytokines in cervical and vaginal fluid. It appears that vaginal homeostasis can be disrupted by two different mechanisms: first, a shift toward increased bacterial diversity can trigger vaginal inflammation, and second, local immunity is altered in some manner, which disrupts the microbiota in response to an environmental change. FRT dysbiosis can have negative effects on reproductive health. This review will examine the increasing evidence for the involvement of the FRT microbiotas and inflammation in gynecologic conditions such as endometriosis, infertility, and endometrial and ovarian cancer; however, the precise mechanisms by which bacteria are involved in these conditions remains speculative at present. While only in their infancy, the use of antibiotics and probiotics to therapeutically alter the FRT microbiota is being studied and is discussed herein. Our current understanding of the intimate relationship between immunity and the FRT microbiota is in its early days, and more research is needed to deepen our mechanistic understanding of this relationship and to assess how our present knowledge can be harnessed to assist in diagnosis and treatment of gynecologic conditions.

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“…bacteria. This finding reflects a substantial role of L. plantarum in infertility management [21,50]. The previous findings were supported by another study of the same group who used the mice model to assess the probiotics effect as a therapeutic alternative to restore fertility.…”

Section: Uses Of Probiotics As Alternative Therapy In Fertility Presementioning

confidence: 56%

“…Probiotics mechanisms of action Lactobacilli spp., as the best-studied probiotic bacteria, display several mechanisms to protect vaginal environment from pathogens. The most significant ones are as follows: [16] production of lactic acid that provides pathogenic exclusion ability by lowering pH to 3.5-4.7 and contribution to indirect pathogenic inhibition by efficient acid cervico-vaginal human mucus ( [17,18] production of bacteriocins which are antimicrobial peptides (AMP) and proteins, produced as a response of imbalance of vaginal microbiome, to protect the host against microbial invasion ( [19,20] blocking the colonization of vaginal pathogens as a result of displacement and exclusion competition by Lactobacilli bacteria [17]; and [21] promotion of immunomodulation mechanisms by provoking innate immunity system, producing H 2 O 2 , and stimulating anti-inflammatory action [22].…”

Section: Main Textmentioning

confidence: 99%

Probiotics and the envisaged role in treating human infertility

Younis

Mahasneh

2020

Middle East Fertil Soc J

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Background Considerable attention is being directed nowadays towards using probiotics as an alternative therapy in treating several human diseases including gastrointestinal tract diseases especially colorectal cancers, cardiovascular diseases, hyperlipidemia, and blood pressure cases. However, infertility as affected by the microbiome and the probable role of probiotics in alleviating infertility problems did not receive the deserved attention, especially in IVF patients with male or female factors. Main body In this review, we tried to draw the attention of researchers in the medical settings to the importance of the forthcoming role of probiotics use in elucidating the role of the microbiome in infertile patients. The hope is to attain the best performance of both male and female reproductive systems and to shed some light on infertility problems. Conclusion More in vivo experiments are still needed to address many aspects of probiotics like proper administration, exact functional strains, required dose, application method, duration of treatment, and combination with antibiotics before considering probiotics as an alternative treatment.

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Potential of Probiotic Lactobacillus plantarum 2621 for the Management of Infertility

Cited by 4 publications

References 28 publications

Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model

Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model

The female reproductive tract microbiotas, inflammation, and gynecological conditions

Probiotics and the envisaged role in treating human infertility

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