Potential of Organic Solvent Tolerant Bacillus sp. BCNU 5006

Abstract

1) In the screening process of organic solvent tolerant bacteria showing good growth in media containing several kinds of organic solvents, one strain was isolated and identified as Bacillus sp. BCNU 5006. The strain was able to tolerate many organic solvents including benzene, toluene, xylene, octane, dodecane, butanol and ethylbenzene. Likewise, it could also utilize these solvents as the sole source of carbon with significant enzyme production. The lipolytic enzyme stability of Bacillus sp. BCNU 5006 was studied in the presence of several kinds of solvents at a 25% (v/v) concentration. The highest enzyme stability was observed in the presence of octane (107%), followed by ethylbenzene (88%), decane (86%), and chloroform (85%). Especially, BCNU 5006 lipase was determined to be more stable than immobilized enzyme (Novozyme 435) in the presence of octane, chloroform and xylene. This organic solvent tolerant Bacillus sp. BCNU 5006 could be expected as a potential bioremediation agent and biocatalyst for biodegradation and provide on organicsolvent-based enzymatic synthetic method in industrial chemical processes.

Solvent Tolerant Bacteria and Their Potential Use

“…BCNU 5005와 Bacillus sp. BCNU 5006도 보고되었다 [8,9]. 이들 국내 토착 유기용매 내성세균 중 P. putida BCNU171는 거의 포화상태의 유기용매 즉 cyclohexane 9 M, xylene 8 M, toluene 9 M, benzene 0.02 M, chloroform 0.04 M 그리고 phenol 0.008 M 에서도 생존 가능 하다고 보고되고 있으며 [7], P. putida BCNU106 는 cyclo- [54].…”