Potential of non-mulberry silk protein fibroin blended and grafted poly(Є-caprolactone) nanofibrous matrices for in vivo bone regeneration

Bhattacharjee, Promita; Naskar, Deboki; Maiti, Tapas K.; Bhattacharya, Debasis; Das, Piyali; Nandi, Samit Kumar; Kundu, Subhas C.

doi:10.1016/j.colsurfb.2016.03.058

Cited by 31 publications

(15 citation statements)

References 35 publications

(44 reference statements)

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“…Another promising ECM for better bone formation would be the inclusion of non-mulberry silk fibroin (NSF) from the tropical tasar silkworm into PCL nanofibers. To study the effectiveness, Bhattacharjee et al investigated the matrices of blended and grafted NSF into and onto electrospun PCL substrates, as an in vivo implant over 60 days [ 27 ]. At the bone defect site in the distal metaphysis region of the rabbits’ femur, the implant showed early bone formation, infiltration, and strong bonding at the bone–implant interface.…”

Section: Silk Materials For Tissue Regenerationsmentioning

confidence: 99%

Tissue Regeneration: A Silk Road

Jao

Mou

2016

JFB

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Silk proteins are natural biopolymers that have extensive structural possibilities for chemical and mechanical modifications to facilitate novel properties, functions, and applications in the biomedical field. The versatile processability of silk fibroins (SF) into different forms such as gels, films, foams, membranes, scaffolds, and nanofibers makes it appealing in a variety of applications that require mechanically superior, biocompatible, biodegradable, and functionalizable biomaterials. There is no doubt that nature is the world’s best biological engineer, with simple, exquisite but powerful designs that have inspired novel technologies. By understanding the surface interaction of silk materials with living cells, unique characteristics can be implemented through structural modifications, such as controllable wettability, high-strength adhesiveness, and reflectivity properties, suggesting its potential suitability for surgical, optical, and other biomedical applications. All of the interesting features of SF, such as tunable biodegradation, anti-bacterial properties, and mechanical properties combined with potential self-healing modifications, make it ideal for future tissue engineering applications. In this review, we first demonstrate the current understanding of the structures and mechanical properties of SF and the various functionalizations of SF matrices through chemical and physical manipulations. Then the diverse applications of SF architectures and scaffolds for different regenerative medicine will be discussed in detail, including their current applications in bone, eye, nerve, skin, tendon, ligament, and cartilage regeneration.

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Section: Silk Materials For Tissue Regenerationsmentioning

confidence: 99%

Tissue Regeneration: A Silk Road

Jao

Mou

2016

JFB

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“…These screws demonstrated good efficacy with no screws failing during implantation and no postoperative adverse events or screw displacements. Histological results demonstrated osteoclast activity, suggesting early resorption of the silk screws; and osteogenesis of the surrounding tissues, indicating continuous bone ingrowth; demonstrating the potential application of silk protein as a bone substitute material [18–20].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional finite element analysis of silk protein rod implantation after core decompression for osteonecrosis of the femoral head

Huang

Chen

Wang

et al. 2019

BMC Musculoskelet Disord

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BackgroundSeveral methods are available for the treatment of early-stage osteonecrosis of the femoral head. Core decompression with implantation is a widely-used treatment. However, no single implant is recognized as the most effective way to prevent disease progression. Silk has high strength and resiliency. This study explored the possibility of a strong and resilient silk protein biomaterial as a new alternative implant.MethodsWe investigated the biomechanical properties of the silk protein material by regular compression, torsion, and three-point bending tests. We established three-dimensional finite element models of different degrees of femoral head osteonecrosis following simple core decompression, fibula implantation, porous tantalum rod implantation, and silk protein rod implantation. Finally, we compared the differences in displacement and surface stress under load at the femoral head weight-bearing areas between these models.ResultsThe elastic modulus and shear modulus of the silk protein material was 0.49GPa and 0.66GPa, respectively. Three-dimensional finite element analyses demonstrated less displacement and surface stress at the femoral head weight-bearing areas following silk protein rod implantation compared to simple core decompression (p < 0.05), regardless of the extent of osteonecrosis. No differences were noted in the surface deformation or surface stress of the femoral head weight-bearing areas following silk protein rod, fibula or tantalum rod implantation (p > 0.05).ConclusionsWhen compared with simple core decompression, silk protein rod implantation demonstrated less displacement and surface stress at the femoral head weight-bearing area, but more than fibula or tantalum rod implantation. Similar effects on the surface stress of the femoral head between the silk rod, fibula and tantalum rod implantations, combined with additional modifiable properties support the use of silk protein as a suitable biomaterial in osteonecrosis surgery.

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“…It also has excellent mechanical property and structural initegrity [20][21][22][23] . These properties of SF make SF-based scaffold have the potential to serve as a bone construct which has already attracted many researchers [23][24][25] . Recently, silk fibers were used as a template for mimicking biomineralization 26) .…”

Section: Introductionmentioning

confidence: 99%

Silk Fibroin Regulates Osteoconduction of Hydroxyapatite in Rat Spine Fusion Model

Wang

Yan

et al. 2019

J. Hard Tissue Biology.

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The purpose of this study was to use a rat spinal fusion model to determine whether osteoconduction of HA is improved by the addition of SF. The SF/HA composite was derived by mixing HA sols with SF sols. In an in vitro study, water absorption testing, cell compatibility and compression test were done. In an in vivo study, 30 S-D male rats had surgery for posterolateral lumbar fusion. Group I rats were implanted with SF/HA composites; Group II rats were implanted with HA; Group III rats were implanted with an autograft. Fusion was evaluated by water absorption test, manual palpation, radiology and histology. The results showed that water absorption was significantly higher with the SF/HA composite compared with HA. Manual palpation and radiology showed a higher rate of fusion in the SF/HA group. Histology also showed better results for the SF/HA group. In, conclusion, SF can modify the osteoconduction of HA. SF/HA composites make good bone graft alternatives in spinal fusion, although additional research needs to be done.

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Potential of non-mulberry silk protein fibroin blended and grafted poly(Є-caprolactone) nanofibrous matrices for in vivo bone regeneration

Cited by 31 publications

References 35 publications

Tissue Regeneration: A Silk Road

Tissue Regeneration: A Silk Road

Three-dimensional finite element analysis of silk protein rod implantation after core decompression for osteonecrosis of the femoral head

Silk Fibroin Regulates Osteoconduction of Hydroxyapatite in Rat Spine Fusion Model

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