Potential of MALDI-TOF mass spectrometry as a rapid detection technique in plant pathology: identification of plant-associated microorganisms

Ahmad, Faheem; Babalola, Olubukola Oluranti; Tak, Hamid Iqbal

doi:10.1007/s00216-012-6091-7

Cited by 44 publications

(38 citation statements)

References 71 publications

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“…If Speclust software were used for the data analysis, the three major peaks should be extracted and displayed as a dendrogram. Major peaks observed in MALDI biotyping were hypothesized to be ribosomal proteins in bacteria . Indeed, the major peaks detected in this study were consistent with ribosomal proteins.…”

Section: Resultssupporting

confidence: 77%

“…Major peaks observed in MALDI biotyping were hypothesized to be ribosomal proteins in bacteria. [14][15][16][17] Indeed, the major peaks detected in this study were consistent with ribosomal proteins.…”

Section: Analysis Of Spider Mitessupporting

confidence: 77%

“…As mentioned above, the major peaks observed here were tentatively thought to represent ribosomal proteins according to other MALDI‐biotyping reports about microorganisms. The culture conditions were not affected so much in their analysis . In this study, Tk and Tu were grown on bean leaves, which is considered their best host material .…”

Section: Resultsmentioning

confidence: 99%

“…The databases, which consist of mass fingerprints mainly collected from microorganisms related to medicine, have become commercially available. Thus, the application of MALDI‐TOFMS to agricultural science was also expected . Plant pathogens can be identified by MALDI biotyping analyses using isolated colonies on culture media.…”

mentioning

confidence: 99%

“…Thus, the application of MALDI-TOFMS to agricultural science was also expected. [17] Plant pathogens can be identified by MALDI biotyping analyses using isolated colonies on culture media. Recently, plant bacteria were directly identified on the infected leaves without the culturing process.…”

mentioning

confidence: 99%

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Mass fingerprint analysis of spider mites (Acari) by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry for rapid discrimination

Kajiwara

Hinomoto

Gotoh

2016

Rapid Comm Mass Spectrometry

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Section: Resultssupporting

confidence: 77%

Section: Analysis Of Spider Mitessupporting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Mass fingerprint analysis of spider mites (Acari) by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry for rapid discrimination

Kajiwara

Hinomoto

Gotoh

2016

Rapid Comm Mass Spectrometry

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Propagation and characterization of viable arbuscular mycorrhizal fungal spores within maize plant (Zea mays L.)

2021

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BACKGROUND: The harmful effect of chemical fertilizer application on human health and the environment as a modern method of meeting the food demand of the increasing world population demands an urgent alternative that is environmentally friendly, which will pose no harm to human health and the environment. Arbuscular mycorrhizal fungi (AMF) are beneficial soil microorganisms that provide various ecological functions in increasing soil fertility and enhancing plant growth. This present study aimed to propagate, characterize and examine the effect of viable arbuscular mycorrhizal fungal spores on maize (Zea mays L) hosts using molecular methods. The propagation of AMF in the host plant using sterile soil and vermiculite was conducted in the greenhouse.RESULT: The effect of AMF inoculation revealed a significant difference (P > 0.05) in maize growth, root colonization and AMF spore count when compared with the control. In all the parameters measured in this study, all the AMF spores propagated had a positive effect on the maize plant over the control, with the highest value mostly recorded in Rhizophagus irregularis AOB1. The molecular characterization of the spore using a specific universal primer for Glomeromycota established the success of the propagation process, which enhanced the classification of the AMF species into Rhizophagus irregularis OAB1, Glomus mosseae OAB2 and Paraglomus occultum OAB3. CONCLUSION: This finding will be a starting point in producing arbuscular mycorrhizal inoculum as a biofertilizer to enhance plant growth promotion.

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Concurrent Proteomic Fingerprinting and Molecular Analysis of Cyathostomins

et al. 2019

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Rapid, cost-effective, efficient, and reliable helminth species identification is of considerable importance to understand host-parasite interactions, clinical disease, and drug resistance. Cyathostomins (Nematoda: Strongylidae) are considered to be the most important equine parasites, yet research on this group is hampered by the large number of 50 morphologically differentiated species, their occurrence in mixed infections with often more than 10 species and the difficulties associated with conventional identification methods. Here, MALDI-TOF MS, previously successfully applied to identify numerous organisms, is evaluated and compared with conventional and molecular genetic approaches. A simple and robust protocol for protein extraction and subsequent DNA isolation allowing molecular confirmation of proteomic findings is developed, showing that MALDI-TOF MS can discriminate adult stages of the two closely related cyathostomin species Cylicostephanus longibursatus and Cylicostephanus minutus. Intraspecific variability of proteomic profiles within morphospecies demonstrated an identification of morphospecies with an accuracy of close to 100%. In contrast, three genospecies within C. minutus and sex-specific profiles within both morphospecies could not be reliably discriminated using MALDI-TOF MS. In conclusion, MALDI-TOF MS complemented by the molecular protocol is a reliable and efficient approach for cyathostomin species identification.

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Potential of MALDI-TOF mass spectrometry as a rapid detection technique in plant pathology: identification of plant-associated microorganisms

Cited by 44 publications

References 71 publications

Mass fingerprint analysis of spider mites (Acari) by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry for rapid discrimination

Mass fingerprint analysis of spider mites (Acari) by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry for rapid discrimination

Propagation and characterization of viable arbuscular mycorrhizal fungal spores within maize plant (Zea mays L.)

Concurrent Proteomic Fingerprinting and Molecular Analysis of Cyathostomins

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