Potential of lactic acid bacteria at regulating Escherichia coli infection and inflammation of bovine endometrium

Genís, Sandra; Bach, À.; Fàbregas, F.; Arı́s, Anna

doi:10.1016/j.theriogenology.2015.09.054

Cited by 34 publications

(37 citation statements)

References 38 publications

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“…In our recent work, various individual strains of LAB were shown to protect against E. coli infection in vitro and reduce inflammation in epithelial cells (Genís et al, 2016). The hypothesis of the present work is that the combination of LAB strains tested in our previous work (Genís et al, 2016) could reduce inflammation and E. coli infection in bovine endometrium. Ex vivo experiments offer a good opportunity to evaluate these effects considering the combination of several cell types and their interactions.…”

Section: Introductionmentioning

confidence: 74%

“…Cells were quantified using a hemocytometer and incubated at 160,000 cells/cm 2 in culture flasks. Epithelial cell phenotype was confirmed by immunofluorescence labeling against anticytokeratin (monoclonal anti-cytokeratin pan antibody produced in mouse, 1:500 dilution; Sigma-Aldrich) of at least 90% of isolated cells as previously shown (Genís et al, 2016). For the immunofluorescence, endometrial primary cells were grown on coverslips and fixed with 4% paraformaldehyde in PBS.…”

Section: Endometrial Primary Culturementioning

confidence: 99%

“…Cells were incubated with 6 replicates O/N with 4 LAB combinations (Table 1) and positive controls (P. acidilactici at a ratio of 25 bacteria/epithelial cell, in the model without basal inflammation, and with L. reuteri at ratio of 2 bacteria/endometrial cell, in the presence of basal inflammation). The basal inflammation was induced with 10 μg/μL of IL-1β as described elsewhere (Genís et al, 2016). Cells were then challenged with 4 × 10 6 cfu/well of E. coli for 6 h. Infection without LAB was used as an infection control and incubation with medium instead of E. coli as a negative control.…”

Section: In Vitro Experiments: Lab Treatment Inflammation and E Comentioning

confidence: 99%

“…Because the traditional antimicrobial treatment applied for metritis may lack efficacy and does not typically improve reproductive performance of the treated animals (Gilbert and Schwark, 1992;Otero et al, 2006), the use of probiotic strains of bacteria has been proposed as an alternative to prevent postpartum uterine infections and inflammation (Ametaj et al, 2014;Deng et al, 2015). In our recent work, various individual strains of LAB were shown to protect against E. coli infection in vitro and reduce inflammation in epithelial cells (Genís et al, 2016). The hypothesis of the present work is that the combination of LAB strains tested in our previous work (Genís et al, 2016) could reduce inflammation and E. coli infection in bovine endometrium.…”

Section: Introductionmentioning

confidence: 99%

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A combination of lactic acid bacteria regulates Escherichia coli infection and inflammation of the bovine endometrium

Genís

Sánchez-Chardi²,

Bach

et al. 2017

Journal of Dairy Science

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Uterine function in cattle is compromised by bacterial contamination and inflammation after calving. The objective of this study was to select a combination of lactic acid bacteria (LAB) to decrease endometrium inflammation and Escherichia coli infection. Primary endometrial epithelial cells were cultured in vitro to select the most favorable LAB combination modulating basal tissue inflammation and E. coli infection. Supernatants were obtained to determine expression of pro-inflammatory cytokines, and E. coli infection was evaluated after harvesting the tissue and plate counting. The selected LAB combination was tested in uterus explants to assess its capacity to modulate basal and acute inflammation (associated with E. coli infection). The combination of Lactobacillus rhamnosus, Pediococcus acidilactici, and Lactobacillus reuteri at a ratio of 25:25:2, respectively, reduced E. coli infection in vitro with (89.77%) or without basal tissue inflammation (95.10%) compared with single LAB strains. Lactic acid bacteria treatment reduced CXCL8 and IL1B expression 4.7- and 2.2-fold, respectively, under acute inflammation. Ex vivo, the tested LAB combination reduced acute inflammation under E. coli infection, decreasing IL-8, IL-1β, and IL-6 up to 2.2-, 2.5-, and 2.2-fold, respectively. In the total inflammation model, the LAB combination decreased IL-8 1.6-fold and IL-6 1.2-fold. Ultrastructural evaluation of the tissue suggested no direct interaction between the LAB and E. coli, although pathological effects of E. coli in endometrial cells were greatly diminished or even reversed by the LAB combination. This study shows the promising potential of LAB probiotics for therapeutic use against endometrial inflammation and infection.

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Section: Introductionmentioning

confidence: 74%

Section: Endometrial Primary Culturementioning

confidence: 99%

Section: In Vitro Experiments: Lab Treatment Inflammation and E Comentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A combination of lactic acid bacteria regulates Escherichia coli infection and inflammation of the bovine endometrium

Genís

Sánchez-Chardi²,

Bach

et al. 2017

Journal of Dairy Science

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“…Supplementation with different sources of selenium in diet before and after calving did not reduce the incidence of SCE at 30 DIM but cows with SCE had lower fertilization rate than healthy cows (Cerri et al, 2009). Some lactic acid bacteria (LAB) have shown to decrease the infection and inflammation in cultured endometrial cells, but more research using in vivo models is needed to see if the uterine infusion of LAB can improve uterine health status (Genis et al, 2016).…”

Section: Treatment Of Uterine Diseasementioning

confidence: 99%

Uterine inflammation affects the reproductive performance of dairy cows: A review

Molina‐Coto

Lucy

2018

Mesoam. Agron.

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Incidence of post-partum uterine disease is an important concern in dairy cattle, because it affects its reproduction. Therefore, the objective of this review of literature was to generate a multifactorial overview about uterine diseases, and the reproductive performance of dairy cows, from a zootechnical approach. Dairy cows face multiple challenges around parturition. Immune suppression around calving, exposition to trauma and uterine bacterial contamination, metabolic diseases, lactation, and changes in management make dairy cows susceptible to uterine diseases. Most cows are able to eliminate uterine infection after calving, however, some cows keep uterine disease. Uterine disease may show clinical signs, but also silent signs that affect fertility as well. Poor reproductive performance is not caused by those signs by themselves, but due to alterations in ovarian and uterine function. Also, the problem of this silent signs is that farmers become aware of the disease when it has already caused negative effects on the reproductive performance. Sometimes, uterine disease is still present at the moment of the first service after calving. Uterine disease make it harder for cows to get pregnant because it affects the establishment and maintenance of pregnancy, being another cause for infertility, increasing the cull rate and decreasing incomes from the dairy industry. ResumenEl ganado lechero, entre otros desafíos, presenta enfermedades uterinas post parto que afectan su reproducción, por tanto, el objetivo de esta revisión de literatura fue generar una visión desde múltiples aristas acerca de las enfermedades uterinas y el desempeño reproductivo de la vaca lechera desde un enfoque zootécnico. La vaca lechera enfrenta múltiples retos en el momento del parto. Inmunosupresión alrededor del parto, exposición a trauma y contaminación uterina, enfermedades metabólicas, lactancia y cambios en el manejo, hacen a la vaca lechera un animal vulnerable de sufrir algún tipo de enfermedad uterina. La mayoría de las vacas pueden eliminar la infección Literature review Agron. Mesoam. 29(2):449-468, mayo-agosto, 2018

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Prebiotic and Probiotic-Based Strategies for the Control of Antimicrobial Resistance

Joseph

Remya

Greeshma

2023

Handbook on Antimicrobial Resistance

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Potential of lactic acid bacteria at regulating Escherichia coli infection and inflammation of bovine endometrium

Cited by 34 publications

References 38 publications

A combination of lactic acid bacteria regulates Escherichia coli infection and inflammation of the bovine endometrium

A combination of lactic acid bacteria regulates Escherichia coli infection and inflammation of the bovine endometrium

Uterine inflammation affects the reproductive performance of dairy cows: A review

Prebiotic and Probiotic-Based Strategies for the Control of Antimicrobial Resistance

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