Potential of Irradiation Technology for Improved Shellfish Sanitation

Mallett, John C.; Beghian, L. E.; Metcalf, Theodore G.

doi:10.1201/9781351074810-24

Cited by 6 publications

(5 citation statements)

References 8 publications

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“…It is also used for pasteurization purpose. D values (1-log reduction) of V. parahaemolyticus have been determined as 0.1 kGy in shrimp (Bandekar et al, 1987) and oysters (Mallett et al, 1991). This D value was similar to the results of the present study ($1-log reduction of V. parahaemolyticus in the oysters and clams using 0.1 kGy).…”

Section: Discussionsupporting

confidence: 89%

Inactivation ofVibrio parahaemolyticusin shucked raw oyster (Grassostrea gigas) and clam (Venerupis phillippinarum) by using a combination of NaClO and gamma irradiation

Park

2017

Food sci. technol. int.

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This study investigated the synergistic effects of sodium hypochlorite (NaClO) and gamma irradiation combination against Vibrio parahaemolyticus in shucked oysters and clams. V. parahaemolyticus decreased to 1.1-5.6 log CFU/g in oysters and 1.1-5.7 logCFU/g in clams by NaClO (20-80 ppm) + γ irradiation (0.1-2.0 kGy) combinations. V. parahaemolyticus was not detected by 60 or 80 ppm NaClO + 2.0 kGy. Synergistic reduction of >1 log was observed by 60 ppm NaClO + 0.3-2 kGy and 80 ppm NaClO + 0.5 or 2 kGy. Specifically, >2 log of the synergistic reduction was obtained by 60 or 80 ppm NaClO + 2 kGy. Furthermore, using the Weibull model, 5D values (5-log reductions) were calculated for 60 or 80 ppm NaClO + 0.5-0.9 kGy. No significant differences were observed for all sensory parameters between samples of 2.0 kGy + 0-80 ppm NaClO. This study suggests that 60 ppm NaClO + 2.0 kGy in reducing 7-log V. parahaemolyticus without any deteriorative changes of sensory qualities could be a potential strategy for post-harvest process in seafood processing and distribution to enhance the microbial safety of molluscan shellfish.

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Section: Discussionsupporting

confidence: 89%

Inactivation ofVibrio parahaemolyticusin shucked raw oyster (Grassostrea gigas) and clam (Venerupis phillippinarum) by using a combination of NaClO and gamma irradiation

Park

2017

Food sci. technol. int.

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“…Previous studies investigating the possible inactivation of RV using γ-rays have focused on food sterilisation. Consequently, the D 10 values reported by these studies were calculated for irradiating RV-contaminated food at room temperature [ 44 ]. These studies did not address the effect of the complex RV genome structure of RV on the inactivation curve of highly concentrated materials.…”

Section: Discussionmentioning

confidence: 99%

Gamma-irradiated rotavirus: A possible whole virus inactivated vaccine

et al. 2018

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Rotavirus (RV) causes significant morbidity and mortality in developing countries, where children and infants are highly susceptible to severe disease symptoms. While live attenuated vaccines are available, reduced vaccine efficacy in developing countries illustrates the need for highly immunogenic alternative vaccines. Here, we studied the possible inactivation of RV using gamma(γ)-irradiation, and assessed the sterility and immunogenicity of γ-irradiated RV (γ-RV) as a novel vaccine candidate. Interestingly, the inactivation curve of RV did not show a log-linear regression following exposure to increased doses of γ-rays, and consequently the radiation dose required to achieve the internationally accepted Sterility Assurance Level could not be calculated. Nonetheless, we performed sterility testing based on serial passages of γ-RV, and our data clearly illustrate the lack of infectivity of γ-RV preparations irradiated with 50 kGy. In addition, we tested the immunogenicity of 50 kGy γ-RV in mice and our data illustrate the induction of strong RV-specific neutralising antibody responses following administration of γ-RV without using adjuvant. Therefore, whilst γ-RV may not constitute a replacement for current RV vaccines, this study represents a proof-of-concept that γ-irradiation can be applied to inactivate RV for vaccine purposes. Further investigation will be required to address whether γ-irradiation can be applied to improve safety and efficacy of existing live attenuated vaccines.

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“…But, there is no report with reference to the effect of EBI on MRSA. In the present study, 1KGy EBI was effectively eliminate this pathogen from shellfish (Mallett et al, 1991).…”

Section: O P ÿmentioning

confidence: 47%