Potential of Immobilized Whole-Cell Methylocella tundrae as a Biocatalyst for Methanol Production from Methane

Mardina, Primata; Li, Jinglin; Patel, Sanjay K.S.; Kim, In-Won; Lee, Jung-Kul; Selvaraj, Chandrabose

doi:10.4014/jmb.1602.02074

Cited by 55 publications

(36 citation statements)

References 32 publications

(52 reference statements)

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“…Encapsulation of M. sporium was performed using Na-alginate and silica gel-based systems. For the Na-alginate bead entrapment method, whole cells were prepared using 2% (w/v) alginate and a loading concentration of 3 mg of DCM/ml was used, as described previously [6,17]. Briefly, washed cells were mixed with Naalginate solution, and the resulting mixture was extruded drop wise using a syringe into 200 ml of 1.5 M CaCl 2 solution for the preparation of beads.…”

Section: Whole Cell Encapsulation Of M Sporiummentioning

confidence: 99%

“…One potential strategy for curbing CH 4 emissions is to transform the gas to methanol, which can then be used as fuel or raw material for the production of organic solvents [5,7,42]. Several efforts have been undertaken to increase the cost effectiveness and sustainability of the bioconversion process, attracting the attention of industries [4,8,9,17,28,29,33,44,45]. Methanotrophs, which utilize CH as the carbon source and act by oxidizing the gas to methanol with methane monooxygenase (MMO), which is then converted to formaldehyde by the activity of methanol dehydrogenase (MDH).…”

Section: Introductionmentioning

confidence: 99%

“…One of the distinctive features of M. sporium, compared with other Methylosinus species, is its ability to produce a watersoluble, brown-black-colored pigment [1]. The inhibitors of MDH, such as phosphate buffer, ammonium chloride, ethylenediaminetetraacetic acid, magnesium chloride (MgCl 2 ), and sodium chloride, offer significant advantages to improve methanol production [4,8,17,28,31,40,43]. Additionally, immobilization is widely used to improve the properties of cells [16-18, 20, 24, 25, 30, 37, 39].…”

Section: Introductionmentioning

confidence: 99%

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Production of Methanol from Methane by Encapsulated Methylosinus sporium

Patel¹,

Jeong²,

Mehariya³

et al. 2016

Journal of Microbiology and Biotechnology

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Massive reserves of methane (CH₄) remain unexplored as a feedstock for the production of liquid fuels and chemicals, mainly because of the lack of economically suitable and sustainable strategies for selective oxidation of CH₄ to methanol. The present study demonstrates the bioconversion of CH₄ to methanol mediated by Type I methanotrophs, such as and. Furthermore, immobilization of a Type II methanotroph, , was carried out using different encapsulation methods, employing sodium-alginate (Na-alginate) and silica gel. The encapsulated cells demonstrated higher stability for methanol production. The optimal pH, temperature, and agitation rate were determined to be pH 7.0, 30°C, and 175 rpm, respectively, using inoculum (1.5 mg of dry cell mass/ml) and 20% of CH₄ as a feed. Under these conditions, maximum methanol production (3.43 and 3.73 mM) by the encapsulated cells was recorded. Even after six cycles of reuse, the Na-alginate and silica gel encapsulated cells retained 61.8% and 51.6% of their initial efficiency for methanol production, respectively, in comparison with the efficiency of 11.5% observed in the case of free cells. These results suggest that encapsulation of methanotrophs is a promising approach to improve the stability of methanol production.

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Section: Whole Cell Encapsulation Of M Sporiummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production of Methanol from Methane by Encapsulated Methylosinus sporium

Patel¹,

Jeong²,

Mehariya³

et al. 2016

Journal of Microbiology and Biotechnology

Self Cite

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“…To date, most studies on the use of methanotrophs for methanol production have used NO 3 − mineral salts (NMS) medium [10], allowing the study of single species of type II methanotrophs [11,12]. Thus, previous studies of methanol production were conducted under limited, controlled laboratory conditions using only single methanotrophic species and pure methane.…”

Section: Introductionmentioning

confidence: 99%

Development of a Combined Aerobic–Anoxic and Methane Oxidation Bioreactor System Using Mixed Methanotrophs and Biogas for Wastewater Denitrification

Kim

Lee

Yoo

et al. 2019

Water

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We developed a lab-scale aerobic–methane oxidation bioreactor (MOB)–anoxic system, combining a MOB and the aerobic–anoxic denitrification process, and evaluated its potential for advanced nitrogen treatment in wastewater treatment plants (WWTPs). The MOB used biogas generated from a WWTP and secondary-treated wastewater to support mixed methanotroph cultures, which mediated the simultaneous direct denitrification by methanotrophs and methanol production necessary for denitrifying bacteria in the anoxic chamber for denitrification. Compared to the aerobic–anoxic process, the aerobic–MOB–anoxic system with an influent concentration of 4.8 L·day−1 showed a marked increase in the reduction efficiency for total nitrogen (41.9% vs. 85.9%) and PO4−3-P (41.1% vs. 69.5%). However, the integrated actions of high nitrogen and phosphorus consumption are required for methanotroph growth, as well as the production and supply of methanol as a carbon source for denitrification and methane monooxygenase-mediated oxidation of NH3 into N2O by methanotrophs. After three months of continuous operation using actual wastewater, the total nitrogen removal rate was 76.3%, equivalent to the rate observed in a tertiary-advanced WWTP, while the total phosphorus removal rate reached 83.7%.

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“…Studies of methanol production using methanotrophs have focused on single species of type II methanotrophs (e.g., Methylosinus trichosporium OB3; OB3b; NCIB) using nitrate mineral salts medium (NMS medium) [5][6][7][8][9][10][11][12][13]. However, the preparation of synthetic culture medium and the use of commercially available media are costly, and both generate chemical wastewater.…”

Section: Introductionmentioning

confidence: 99%

Bio-Methanol Production Using Treated Domestic Wastewater with Mixed Methanotroph Species and Anaerobic Digester Biogas

Kim

Yoo

Yoon

et al. 2018

Water

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The development of cost-effective methods, which generate minimal chemical wastewater, for methanol production is an important research goal. In this study, treated wastewater (TWW) was utilized as a culture solution for methanol production by mixed methanotroph species as an alternative to media prepared from commercial or chemical agents, e.g., nitrate mineral salts medium. Furthermore, a realistic alternative for producing methanol in wastewater treatment plants using biogas from anaerobic digestion was proposed. By culturing mixed methanotroph species with nitrate and phosphate-supplemented TWW in municipal wastewater treatment plants, this study demonstrates, for the first time, the application of biogas generated from the sludge digester of municipal wastewater treatment plants. NaCl alone inhibited methanol dehydrogenase and the addition of 40 mM formate as an electron donor increased methanol production to 6.35 mM. These results confirmed that this practical energy production method could enable cost-effective methanol production. As such, methanol produced in wastewater treatment plants can be used as an eco-friendly energy and carbon source for biological denitrification, which can be an alternative to reducing the expenses required for the waste water treatment process.

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Potential of Immobilized Whole-Cell Methylocella tundrae as a Biocatalyst for Methanol Production from Methane

Cited by 55 publications

References 32 publications

Production of Methanol from Methane by Encapsulated Methylosinus sporium

Production of Methanol from Methane by Encapsulated Methylosinus sporium

Development of a Combined Aerobic–Anoxic and Methane Oxidation Bioreactor System Using Mixed Methanotrophs and Biogas for Wastewater Denitrification

Bio-Methanol Production Using Treated Domestic Wastewater with Mixed Methanotroph Species and Anaerobic Digester Biogas

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