Potential of Pseudomonas chlororaphis subsp. aurantiaca Strain Pcho10 as a Biocontrol Agent Against Fusarium graminearum

Abstract

To develop an effective biocontrol strategy for management of Fusarium head blight on wheat caused by Fusarium graminearum, the bacterial biocontrol agent Pcho10 was selected from more than 1,476 wheat-head-associated bacterial strains according to its antagonistic activity in vitro. This strain was subsequently characterized as Pseudomonas chlororaphis subsp. aurantiaca based on 16S ribosomal DNA sequence analysis, assays of the BIOLOG microbial identification system, and unique pigment production. The major antifungal metabolite produced by Pcho10 was further identified as phenazine-1-carboxamide (PCN) on the basis of nuclear magnetic resonance data. The core PCN biosynthesis gene cluster in Pcho10 was cloned and sequenced. PCN showed strong inhibitory activity against F. graminearum conidial germination, mycelial growth, and deoxynivalenol production. Tests both under growth chamber conditions and in field trials showed that Pcho10 well colonized on the wheat head and effectively controlled the disease caused by F. graminearum. Results of this study indicate that P. chlororaphis subsp. aurantiaca Pcho10 has high potential to be developed as a biocontrol agent against F. graminearum. To our knowledge, this is the first report of the use of P. chlororaphis for the management of Fusarium head blight.

Green-Synthesization of Silver Nanoparticles Using Endophytic Bacteria Isolated from Garlic and Its Antifungal Activity against Wheat Fusarium Head Blight Pathogen Fusarium graminearum

“…Furthermore, the formation of AgNPs was confirmed by the results of UV spectrometers, which showed a spectrum of surface plasmon resonance (SPR) at the 422 nm absorption band ( Figure 1). Interestingly, the result of this study is consistent with the spectra contained in the literature regarding silver nanoparticles [20,22,27,28].…”

“…F. graminearum strain PH−1 [20] was kindly provided by Professor Yanni Yin in the Institute of Biotechnology, Zhejiang University, Hangzhou, China. The endophytic bacterium P. poae strain CO was isolated from fresh leaves of a garlic plant grown in Hangzhou, Zhejiang Province, China.…”

“…The functional group of the biosynthesized AgNPs with antifungal activity was determined by Fourier transform infrared spectroscopy (FTIR), which was carried out as described by Hossain et al [20]. In brief, 1 mg of freeze-dried AgNP powders were mixed with KBr (300 mg), and the FTIR was recorded in the spectral range of 500-4000 cm by using an AVATAR 370 FTIR spectrometer (Thermo Nicolet, MA, USA).…”

“…In brief, 1 mg of freeze-dried AgNP powders were mixed with KBr (300 mg), and the FTIR was recorded in the spectral range of 500-4000 cm by using an AVATAR 370 FTIR spectrometer (Thermo Nicolet, MA, USA). Furthermore, the crystalline nature of the biosynthesized AgNPs was analyzed by X-ray diffraction (XRD), as described by Hossain et al [20] by using an XPert PRO diffractometer (Holland) with a detector voltage of 45 kV and a current of 40 mA while using CuKo radiation. In addition, the morphology of the biosynthesized AgNPs was recorded through the observation of both TEM (JEM−1230, JEOL, Tokyo, Japan) and SEM (SEM, TM−1000, Hitachi, Japan), which were performed according to the method of [18].…”

Benzoquinone from Fusarium pigment inhibits the proliferation of estrogen receptor-positive MCF-7 cells through the NF-κB pathway via estrogen receptor signaling

“…Biosynthetic pigments are important natural pigments, not restricted by seasons, materials and other conditions. Microorganisms can undergo continuous fermentation to produce pigments to meet the increasing needs of the community, and the majority of microbial pigments have biological activities (4,5). …”