Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery

Abstract

The aim of this study was to characterize and compare selected Lactobacillus strains originating from different environments (cow milk and hen feces) with respect to their applicative potential to colonize gastrointestinal track of chickens before hatching from an egg. In vitro phenotypic characterization of lactobacilli strains included the investigation of the important prerequisites for persistence in gastrointestinal tract, such as a capability to survive in the presence of bile salts and at low pH, enzymatic and sugar metabolic profiles, adhesion abilities, and resistance to osmolytes, temperature, and antibiotics. Regarding the resistance of lactobacilli to most of the various stress factors tested, the milk isolate Lactobacillus plantarum IBB3036 showed better abilities than the chicken feces isolate Lactobacillus salivarius IBB3154. However, regarding the acidification tolerance and adherence ability, L. salivarius IBB3154 revealed better characteristics. Use of these two selected lactobacilli isolates together with proper prebiotics resulted in the preparation of two S1 and S2 bioformulations, which were injected in ovo into hen Cobb500 FF fertilized eggs. Furthermore, in vivo tests assessing the persistence of L. plantarum IBB3036 and L. salivarius IBB3154 in the chicken gastrointestinal tract was monitored by PCR-based classical and quantitative techniques and revealed the presence of both strains in fecal samples collected 3 days after hatching. Subsequently, the number of L. salivarius IBB3154 increased significantly in the chicken intestine, whereas the presence of L. plantarum IBB3036 was gradually decreased.

Genomics Analysis of Bacillus megaterium 1259 as a Probiotic and Its Effects on Performance in Lactating Dairy Cows

“…They play a vital role in animal production due to their non-residual and non-polluting nature. Previous studies have reported the effects of different probiotics in animal diets, such as enhancing the growth rate, increasing feed intake and feed efficiency, improving carcass yield and quality, increasing nutrient digestibility, and controlling enteric pathogens [ 2 , 3 , 4 , 5 , 6 ]. Bacillus , spore-forming bacteria, are becoming increasingly popular as probiotics used in animal feed.…”

In vitro assessment of potential probiotic characteristics of indigenous Lactococcus lactis and Weissella oryzae isolates from rainbow trout ( Oncorhynchus mykiss Walbaum)

“…The probiotic bacteria should be tolerant to stress factors such as salt and temperature changes, which affect the viability of the bacterial cell. Therefore, the ability to grow in different ranges of salinity and temperature is a positive property in LAB (Aleksandrzak Piekarczyk et al 2018). Hagi et al .…”

“…After incubating at 37°C for 3 h, OD 630 was recorded, and the result was expressed based on growth rate (Hosseini et al 2009; Sharifuzzaman et al 2018). To evaluate the effect of salinity and different temperatures, selected LAB isolates were spotted on MRS agar plates containing 2, 4, 6 and8 % (w/v) sodium chloride and incubation at 4, 15, 25, 37 and 45°C for 24 h respectively (Aleksandrzak Piekarczyk et al 2018).…”

In vitro assessment of safety and functional probiotic properties of Lactobacillus mucosae strains isolated from Iranian native ruminants intestine

“…The effects of different temperatures and sodium chloride concentrations on bacterial survivability were examined according to the procedure described by Mortezaei et al (2020) and Aleksandrzak-Piekarczyk et al (2019), respectively. During temperature treatments, each selected isolate was exposed to different temperatures (37, 45 and 50 C) for 48 h and formation of bacterial colonies was evaluated.…”

“…Based on the selection criteria for probiotic strains, a bacterial strain should be able to withstand low gastric pH and bile salts of the intestine, adhere to the intestinal mucosa and exceed safety criteria, such as the absence of haemolytic activity and sensitivity to antimicrobials of human and veterinary importance (Musikasang et al 2009;Iñiguez-Palomares et al 2011). Besides, resistance to osmolytes and temperature is considered as notable features that lead to the successful performance of probiotic bacteria (Salas-Jara et al 2016;Aleksandrzak-Piekarczyk et al 2019) and those candidates which meet the established criteria for probiotic can be used to produce probiotic supplements.…”