Potential of Entomopathogenic Bacteria and Fungi

Sharma, Latika; Bohra, Nitin; Singh, Rupesh Kumar; Marques, Guilhermina

doi:10.1007/978-3-030-23045-6_4

Cited by 6 publications

(9 citation statements)

References 152 publications

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“…Some EPF, such as Beauveria and Lecanicillium W.Gams & Zare (Hypocreales: Cordycipitaceae) are also considered as plant disease (powdery mildew, rusts etc.) antagonists, acting through competition for resources, antibiosis, systemic resistance, or even parasitism against soil fungi [ 62 , 63 , 64 ]. Hence, such interaction with soil micro-organisms should be examined prior to EPF applications.…”

Section: Discussionmentioning

confidence: 99%

Effect of Soil Chemical Properties on the Occurrence and Distribution of Entomopathogenic Fungi in Portuguese Grapevine Fields

et al. 2021

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Entomopathogenic fungi (EPF) contribute to different ecosystem services. However, factors affecting their natural occurrences in soil remain poorly understood. In a previous study, 81 soil samples were subjected to insect baiting using Galleria mellonella and Tenebrio molitor to isolate EPF from Portuguese vine farms. Here, soils yielding any of the four common EPF, i.e., Beauveria bassiana, Purpureocillium lilacinum, Metarhizium robertsii, and Clonostachys rosea f. rosea, were correlated with their chemical properties. Beauveria bassiana was negatively affected by higher available P (p = 0.02), exchangeable K-ions (p = 0.016) and positively affected by higher soil pH_H2O (p = 0.021). High exchangeable K-ions inhibited P. lilacinum (p = 0.011) and promoted C. rosea f. rosea (p = 0.03). Moreover, high available K also suppressed P. lilacinum (p = 0.027). Metarhizium robertsii was inhibited by higher organic matter content (p = 0.009), higher C:N (p = 0.017), total N (p = 0.007), and exchangeable Mg-ions (p = 0.026), and promoted by higher exchangeable Na-ions (p = 0.003). Nonetheless, mean comparisons and principal component analysis suggested that higher soil pH and exchangeable Ca-ions have contrasting effects on EPF occurrences, as they promote B. bassiana and inhibit M. robertsii. Herbicides did not seem to affect EPF presence. Overall, this study is among the first reports on the effects of soil chemistry on EPF other than Metarhizium, and will facilitate biological pest management approaches.

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Section: Discussionmentioning

confidence: 99%

Effect of Soil Chemical Properties on the Occurrence and Distribution of Entomopathogenic Fungi in Portuguese Grapevine Fields

et al. 2021

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“…Moreover, they can also be isolated from soils or phylloplane as they spend a considerable part of their life as saprophytes in soils or as plant endophytes. However, to our knowledge, their survival as soil saprophytes has not been proven yet [ 4 , 5 , 6 , 7 , 8 , 35 , 36 ]. In either case, the material can be cultured directly onto a medium selective for an EPF or the material can be baited with an infection-sensitive insect [ 37 ].…”

Section: Isolation Of Entomopathogenic Fungimentioning

confidence: 99%

“…Entomopathogenic bacteria (EPB) and entomopathogenic fungi (EPF) are the natural enemies of insect-pests. Hence, their importance in agriculture is quite high [ 4 , 5 , 6 , 7 , 8 ]. The majority of the EPB belong to a few bacterial families, such as Bacillaceae, Enterobacteriaceae, Micrococcaceae, Pseudomonadaceae, and Streptococcaceae.…”

Section: Introductionmentioning

confidence: 99%

Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi

et al. 2020

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Entomopathogenic bacteria and fungi are quite frequently found in soils and insect cadavers. The first step in utilizing these microbes as biopesticides is to isolate them, and several culture media and insect baiting procedures have been tested in this direction. In this work, the authors review the current techniques that have been developed so far, in the last five decades, and display brief protocols which can be adopted for the isolations of these entomopathogens. Among bacteria, this review focuses on Serratia spp. and bacteria from the class Bacilli. Among fungi, the review focuses those from the order Hypocreales, for example, genera Beauveria, Clonostachys, Lecanicillium, Metarhizium, and Purpureocillium. The authors chose these groups of entomopathogenic bacteria and fungi based on their importance in the microbial biopesticide market.

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“…Indeed, the soil is a reservoir of numerous microorganisms critical for the sustainable functioning of natural and managed ecosystems (Sharma et al, 2019). Several natural rhizobacteria inhabiting the rhizospheric soil exist, their application reduces the utilization of chemical fertilizers, pesticides and other artificial growth regulators that cause severe health and environmental issues, soil infertility, water pollution and biodiversity losses (Singh et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Multiple species of entomopathogenic bacteria may be isolated from this part of soil, they are the enemies of pest-insects, whose utility in agroecosystems has been studied since decades. These entomopathogens spend significant time period in soil, either as saprotrophs, resting spores or dormant endospores (Oulebsir-Mohandkaci et al, 2016;Sharma et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Phylogeny and Molecular Study of Some Entomopathogenic Rhizobacteria Isolated from Two Regions in Algeria

Oulebsir-Mohandkaci¹,

Farida²,

Khemili³

et al. 2019

Current Research in Bioinformatics

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The use of bacteria in the control of insect pests is a form of biological control whose practice is still not widespread. It is in this context that the present work falls. It concerns the isolation, characterization and identification of local bacterial strains for the purpose of their use in the control of certain pests. Indeed, 20 bacteria were isolated from soil cultivated in the region of Boumerdes (center of Algeria) with a total of 21 bacterial strains isolated from Adrar region (Desert Algerian). After carrying out the efficacy tests against 2 insect pests; Migratory locust (Locusta migratoria) and wax moth (Galleria mellonella), 8 potentially interesting strains were identified based on their genetic traits. Molecular characterization of these strains was performed by isolation of DNA, PCR and sequencing of the 16S rRNA gene, followed by phylogenetic analysis. The rDNA16S sequences of the 8 strains named B1, B2, B3, B4, B5, B6, H1 and H2 were recorded in the EMBL/EBI database and their phylogenetic analysis revealed that they belong to the genera Bacillus, Pseudomonas, Enterobacter and Delftia with a very high percentage of similarity with Bacillus thuringiensis strains (NR_043403) (99%) for isolate B1, Bacillus weihenstephanensis (NR_024697) (99%) for isolate B2, Pseudomonas fragi (JCM5420) (99%) for isolates B3 and B4, Bacillus thuringiensis (CMBLBT-5) (99%) for isolate B4, Enterobacter ludwigii (EN-119) for B5, Bacillus thuringiensis (4916) (99%) for isolate H1 and Delftia lacustris (R-54734) (100%) for isolate H2.

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Potential of Entomopathogenic Bacteria and Fungi

Cited by 6 publications

References 152 publications

Effect of Soil Chemical Properties on the Occurrence and Distribution of Entomopathogenic Fungi in Portuguese Grapevine Fields

Effect of Soil Chemical Properties on the Occurrence and Distribution of Entomopathogenic Fungi in Portuguese Grapevine Fields

Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi

Phylogeny and Molecular Study of Some Entomopathogenic Rhizobacteria Isolated from Two Regions in Algeria

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