Potential of different Xanthomonas campestris strains for xanthan biosynthesis on waste glycerol from biodiesel production

Rončević, Zorana; Zahović, Ida; Danilović, Nikolina; Dodić, Siniša; Grahovac, Jovana; Dodić, Jelena

doi:10.5937/jpea24-25506

Cited by 5 publications

(9 citation statements)

References 18 publications

(22 reference statements)

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“…After the cultivation, xanthan concentration in the medium was 11.10 g/L. This value is higher compared to the values obtained in previous researches, where xanthan was produced by Xanthomonas strains, isolated from pepper leaves and crucifers on a crude glycerol-based medium, but in smaller volumes (300 mL Erlenmeyer flasks and 2.0 L Woulff bottles), and where xanthan concentration in media varied from around 5.00 g/L to 8.00 g/L (Rončević, Zahović, et al 2020;Zahović et al 2022;. Moreover, xanthan concentration obtained in this study is also higher in comparison with the results obtained when reference strain Xanthomonas campestris ATCC 13951 was cultivated on a medium containing crude glycerol (15.00 g/L).…”

Section: Results and Disscusioncontrasting

confidence: 59%

XANTHAN PRODUCTION ON CRUDE GLYCEROL BY LAB-SCALE BIOREACTOR CULTIVATION OF LOCAL Xanthomonas ISOLATE

Zahović,

Dodić,

Vučurović

et al. 2022

J. eng. process. manag.

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Intensive development of the global biodiesel industry has led to the generation of a large excess of crude glycerol, which is impure, and its disposal into the environment is unacceptable without previous purification. Since purification costs are high, the application of crude glycerol in biotechnological production of valueadded products represents a promising solution for a sustainable utilization of this effluent. The aim of this study was to examine xanthan biosynthesis by the Xanthomonas PL 3 strain on a medium containing crude glycerol from biodiesel production in a laboratory-scale bioreactor in order to further scale up this bioprocess. Xanthan was produced by submerged cultivation in a crude glycerol-based medium (glycerol content of 20 g/L) in a 3 L lab-scale bioreactor (working volume of 2 L), under aerobic conditions for 168 h (0-48 h: 25°C, 1 vvm and 200 rpm; 48-168 h: 30 °C, 2 vvm, and agitation rate adjusted according to the dissolved oxygen concentration which was maintained at values higher than 30%). The bioprocess was monitored by the analysis of cultivation medium samples in predetermined time intervals, and its success was estimated based on the xanthan concentration in the medium, separated biopolymer average molecular weight and degree of nutrient conversion. In the applied experimental conditions, 11.10 g/L of xanthan with the average molecular weight of 2.85·105 g/moL was biosynthesized. At the end of this bioprocess, the degree of total glycerol, nitrogen and phosphorous conversion was 62.82%, 41.54% and 24.80%, respectively. Results obtained in this study suggest that the Xanthomonas PL 3 strain has the ability to produce xanthan of good quality on cultivation media containing crude glycerol from the biodiesel industry.

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Section: Results and Disscusioncontrasting

confidence: 59%

XANTHAN PRODUCTION ON CRUDE GLYCEROL BY LAB-SCALE BIOREACTOR CULTIVATION OF LOCAL Xanthomonas ISOLATE

Zahović,

Dodić,

Vučurović

et al. 2022

J. eng. process. manag.

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“…In that research, both, inoculum I and inoculum II were incubated for 48 h and the value of xanthan concentration in cultivation medium at the end of biosynthesis was in the range from 6.58 g/L to 8.50 g/L. The value of xanthan concentration in production medium at the end of biosynthesis predicted in this study is also higher than values of xanthan content obtained in another research where Xanthomonas strains isolated from different cruciferous plants were cultivated on crude glycerol containing medium (24). Inoculum I and inoculum II used in that study were incubated for 48 h each and xanthan yield obtained under applied experimental conditions was in the range from 5.22 ± 0.21 g/L to 7.67 ± 0.53 g/L.…”

Section: Figure 1 Pareto Chart Of Standardized Effects Of Incubation ...contrasting

confidence: 66%

“…The results obtained in this research suggest that, in order to achieve the highest concentration of xanthan in the production medium at the end of the bioprocess performed under applied experimental conditions, it is necessary to incubate inoculum I for 36 h and inoculum II for 48 h. This indicate that incubation time of inoculum I can be reduced for 25% comparing to the incubation time of inoculum I from previous studies (23,24) with an increase in xanthan yield for more than 20%. Hence, this research is of great importance for reduction of xanthan production costs and minimization of the negative impact of crude glycerol from biodiesel industry on the environment.…”

Section: Optimization Of Time For Inoculum Preparationmentioning

confidence: 62%

Xanthan production on crude glycerol-based medium by local Xanthomonas isolate: Optimization of inoculum incubation time

2022

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This study is concerned with the optimization of inoculum preparation for xanthan production on crude glycerol-based medium in terms of incubation time of the Xanthomonas strain isolated from infected pepper leaves. Experiments within this research were performed according to 3-level factorial design in order to evaluate the effects of varied parameters, i.e. incubation time of inoculum I (24-48 h) and incubation time of inoculum II (24- 48 h) on xanthan concentration in production medium at the end of bioprocess. Inoculum preparation was performed in two steps (inoculum I and inoculum II) under aerobic conditions, at 25 ?C and 150 rpm. Xanthan biosynthesis was performed at a laboratory level under aerobic conditions at 30 ?C and 150 rpm for 168 h. The quality of the prepared inoculum was assessed indirectly, based on xanthan concentration in the production medium at the end of the bioprocess. Response surface methodology was used to define mathematical model, which describes the individual and interactive effects of examined parameters on the xanthan quantity, while desirability function approach was applied to optimize their values. The obtained results suggest that applied strain is the most productive if the incubation time of inoculum I is between 32 h and 40 h, and the incubation time of inoculum II is in the range from 40 h to 48 h. The developed model predicts that the maximum xanthan concentration of about 10.5 g /L can be achieved if the incubation times of inoculum I and inoculum II are 36 h and 48 h, respectively.

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“…One of economically and environmentally acceptable solution is potential use of crude glycerol as a raw material in biotechnological production [5,6]. Several studies indicate that certain strains of bacteria of the genus Xanthomonas possess the ability to biosynthesize xanthan on a medium containing crude glycerol as the only carbon source [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…This indicates that there is a need for isolation of new Xanthomonas strains able to metabolize glycerol and produce xanthan. Due to this reason, other Xanthomonas strains, rather than reference strain, were used in previous studies focused on xanthan production on glycerol-based media [8,9,15,16].…”

Section: Introductionmentioning

confidence: 99%

Screening of Local Wild Xanthomonas Species for Xanthan Production on Crude Glycerol-based Medium

Zahović

Dodić²,

Grahovac³

et al. 2022

Period. Polytech. Chem. Eng.

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In this study, the effect of cultivation time on xanthan biosynthesis by different Xanthomonas campestris and Xanthomonas euvesicatoria strains, isolated from crucifers and pepper leaves, respectively, was examined. Xanthan was produced by submerged cultivation on crude glycerol-based medium at a laboratory level under aerobic conditions at 30 °C and 150 rpm for 168 h and 240 h. Bioprocess efficacy was estimated based on the xanthan concentration in media at the end of bioprocess and its average molecular weight. According to the obtained results, Xanthomonas strains have statistically significant effect on xanthan concentration in cultivation media when biosynthesis is performed by X. euvesicatoria strains, and cultivation time has significant effect on this parameter only when bioprocess is performed by X.campestris strains. The combination of Xanthomonas strains and cultivation time has a statistically significant effect on xanthan concentration in medium for both groups of isolates. The obtained results show that all applied Xanthomonas strains and cultivation time as well as their combination have statistically significant effect on average molecular weight of xanthan produced in applied experimental conditions. It is found that X. euvesicatoria strains produce higher amount of xanthan in a shorter period of time (168 h) when compared to the X.campestris strains. Xanthan of higher average molecular weight was produced when cultivation of both groups of isolates was performed for 240 h in applied experimental conditions. Results obtained in this research suggest that X. euvesicatoria strains have the greatest potential for application in biotechnological production of xanthan on crude glycerol-based medium.

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Potential of different Xanthomonas campestris strains for xanthan biosynthesis on waste glycerol from biodiesel production

Cited by 5 publications

References 18 publications

XANTHAN PRODUCTION ON CRUDE GLYCEROL BY LAB-SCALE BIOREACTOR CULTIVATION OF LOCAL Xanthomonas ISOLATE

XANTHAN PRODUCTION ON CRUDE GLYCEROL BY LAB-SCALE BIOREACTOR CULTIVATION OF LOCAL Xanthomonas ISOLATE

Xanthan production on crude glycerol-based medium by local Xanthomonas isolate: Optimization of inoculum incubation time

Screening of Local Wild Xanthomonas Species for Xanthan Production on Crude Glycerol-based Medium

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