Potential of desiccation-tolerant plant growth-promoting rhizobacteria in growth augmentation of wheat (Triticum aestivum L.) under drought stress

Shankar, Ajay; Prasad, V.S.S.

doi:10.3389/fmicb.2023.1017167

Cited by 5 publications

(5 citation statements)

References 77 publications

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“…As functions of symbiosis of mycorrhizal fungi (Chandrasekaran, 2022;Begum et al, 2023;Zou et al, 2023), plant growth-promoting rhizobacteria (PGPR) (Shankar and Prasad, 2023;Tanveer et al, 2023;Zhao et al, 2023), and dark septate endophytes (DSE) (Li et al, 2022;He C. et al, 2022;Liu et al, 2023) during drought stress, root endophytic fungi also play important roles in increasing tolerance to drought stress of their host plants (Azevedo et al, 2021;Manzur et al, 2022;Zuo et al, 2022). S. indica is also a root endophytic fungus, and it improves drought tolerance of their host plants, as described above.…”

Section: Discussionmentioning

confidence: 99%

Colonization of root endophytic fungus Serendipita indica improves drought tolerance of Pinus taeda seedlings by regulating metabolome and proteome

Wu,

Yang,

Wang

et al. 2024

Front. Microbiol.

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Pinus taeda is an important forest tree species for plantations because of its rapid growth and high yield of oleoresins. Although P. taeda plantations distribute in warm and wet southern China, drought, sometime serious and long time, often occurs in the region. To explore drought tolerance of P. taeda and usage of beneficial microorganisms, P. taeda seedlings were planted in pots and were inoculated with root endophytic fungus Serendipita indica and finally were treated with drought stress for 53 d. Metabolome and proteome of their needles were analyzed. The results showed that S. indica inoculation of P. taeda seedlings under drought stress caused great changes in levels of some metabolites in their needles, especially some flavonoids and organic acids. Among them, the levels of eriocitrin, trans-aconitic acid, vitamin C, uric acid, alpha-ketoglutaric acid, vitamin A, stachydrine, coumalic acid, itaconic acid, calceolarioside B, 2-oxoglutaric acid, and citric acid were upregulated more than three times in inoculated seedlings under drought stress, compared to those of non-inoculated seedlings under drought stress. KEGG analysis showed that some pathways were enriched in inoculated seedlings under drought stress, such as flavonoid biosynthesis, ascorbate and aldarate metabolism, C5-branched dibasic acid metabolism. Proteome analysis revealed some specific differential proteins. Two proteins, namely, H9X056 and H9VDW5, only appeared in the needles of inoculated seedlings under drought stress. The protein H9VNE7 was upregulated more than 11.0 times as that of non-inoculated seedlings under drought stress. In addition, S. indica inoculation increased enrichment of water deficient-inducible proteins (such as LP3-1, LP3-2, LP3-3, and dehydrins) and those involved in ribosomal structures (such as A0A385JF23). Meanwhile, under drought stress, the inoculation caused great changes in biosynthesis and metabolism pathways, mainly including phenylpropanoid biosynthesis, cutin, suberine and wax biosynthesis, and 2-oxocarboxylic acid metabolism. In addition, there were positive relationships between accumulation of some metabolites and enrichment of proteins in P. taeda under drought stress. Altogether, our results showed great changes in metabolome and proteome in inoculated seedlings under drought stress and provided a guideline to further study functions of metabolites and proteins, especially those related to drought stress.

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Section: Discussionmentioning

confidence: 99%

Colonization of root endophytic fungus Serendipita indica improves drought tolerance of Pinus taeda seedlings by regulating metabolome and proteome

Wu,

Yang,

Wang

et al. 2024

Front. Microbiol.

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“…Meanwhile, rhizobia isolates explored by Kapembwa et al [35] reported that, 88 % had an entire edge, 10 % undulate and 2 % lobate. The rhizobacterial isolates found by Shankar and Prasad [36] had flat, raised, convex, crateriform, or umbonate elevations. In addition, based on the exploration of Odori et al [37], rhizobia colonies that they found appeared mucoid or glistening.…”

Section: Macroscopic Characterizationmentioning

confidence: 93%

Bioprospecting of Rhizobia as Plant Growth Promoting Rhizobacteria Potential from Root Nodules of Groundnut (Arachis hypogaea L.)

Wulandari,

Baskoro,

Mahmuudah

et al. 2024

Trends Sci

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Rhizobia are bacteria that symbiosis with host plant, as shown in the root nodules formation, and provide nitrogen that can be absorbed by plants in greater quantities than rhizobacteria. Available Nitrogen, which absorbed by plants, is the essential requirement for plant growth because its role in increasing yield and quality, hence it is needed in greater quantities than other nutrients. The study aimed to determine the macroscopic and microscopic diversity of rhizobia isolates from the groundnut nodules and their potential as PGPRs, and to identify 16S rRNA isolates with the best potential as PGPRs molecularly. The methods used were isolation from root nodules, screening of PGPR potential, molecular identification based on the 16S rRNA gene, and phylogenetic analysis to determine their kinship. Based on the isolation results, 17 Gram-negative isolates were obtained white to pink or orange color on AG media with various colony characteristics in terms of shape, margin, elevation, and texture. KT 20, which was selected as rhizobia isolate with the best potential as PGPR, has ammonium concentration of 23.12 ppm, synthesizes IAA with a concentration of 10.36 ppm, and phosphates solubilization activity, although its ability to synthesize proteases is low. The results of molecular identification of 16S rRNA showed that KT 20 belongs to the Rhizobium genus with a similarity of 99.48 % and bootstrap value of 96 %. HIGHLIGHTS Isolate KT 20 (identified rhizobia) has excellent ability in fixing nitrogen, which plants needs the most and legumes requires in large number Beside the ability of Isolate KT 20 to fix nitrogen, it also able to synthesize IAA, solubilize phosphate, and synthesize protease The ability of isolate KT 20 as PGPR was equal or better than other rhizobacteria that has been analyzed in other in vitro studies GRAPHICAL ABSTRACT

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“…Also, PGP bacteria increases antioxidant levels, synthesis of ACC deaminase, exopolysaccharides production and ionic homeostasis, which in turn enhances agricultural productivity even in saline-alkaline soil (Mishra et al, 2023). Currently, studies in various parts of the world are reporting improvements in the growth and production of crops such as tomatoes (Fan et al, 2016;Astorga-Elóet al, 2021), maize (Ojuederie and Babalola, 2023), and wheat (Shankar and Prasad, 2023), attribute to the use of PGP bacteria. The utilization of PGP bacteria as inoculants has substantial potential for application in farming, and lowering the rate of chemical fertilizers (Bittencourt et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Mangrove sediments-associated bacterium (Bacillus sp. SW7) with multiple plant growth-promoting traits promotes the growth of tomato (Solanum Lycopersicum)

Afridi,

Sadaiappan,

Nassar

et al. 2024

Front. Mar. Sci.

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Global food production intensification presents a major hurdle to ensuring food security amidst a growing world population. Widespread use of chemical fertilizers in recent decades has risked soil fertility, compounded by the challenges posed by climate change, particularly in arid regions. To address these issues, adopting plant growth-promoting (PGP) bacteria stands out as a promising solution, offering multifaceted benefits to arid agroecosystems. We isolated a bacterial strain, SW7, from mangrove sediment, characterised the entire genome followed by phylogenetic analyses, and evaluated its in-vitro PGP activity. Subsequently, we examined its impact on tomato seed germination and plant growth. The strain SW7 exhibited growth on 11% NaCl, survival at 50°C, and possessed multiple PGP traits such as significant increase in seed germination rate (60.60 ± 38.85%), phosphate (83.3 g L−1) and potassium (39.6 g L−1) solubilization and produced indole acetic acid (3.60 ppm). Additionally, strain SW7 tested positive for ammonia, catalase, and oxidase enzyme production. The strain SW7 genome consists of 5.1 MB with 35.18% G+C content. Through genome-based phylogenetic and orthoANI analyses, the strain was identified as a novel Bacillus species, designated herein as Bacillus sp. SW7. In an eight-week shade-house experiment, inoculation of strain SW7 improved, leaf number, leaf density, leaf area index and mass water of tomatoes. Additional parameters, like chlorophyll a, chlorophyll b and carotenoids were not affected in SW7-inoculated tomatoes. In conclusion, Bacillus sp. SW7 exhibits multiple PGP traits and an adaptive capacity to high temperature and salinity, positioning it as a potential candidate for elevating the productivity of arid agroecosystems.

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Potential of desiccation-tolerant plant growth-promoting rhizobacteria in growth augmentation of wheat (Triticum aestivum L.) under drought stress

Cited by 5 publications

References 77 publications

Colonization of root endophytic fungus Serendipita indica improves drought tolerance of Pinus taeda seedlings by regulating metabolome and proteome

Colonization of root endophytic fungus Serendipita indica improves drought tolerance of Pinus taeda seedlings by regulating metabolome and proteome

Bioprospecting of Rhizobia as Plant Growth Promoting Rhizobacteria Potential from Root Nodules of Groundnut (Arachis hypogaea L.)

Mangrove sediments-associated bacterium (Bacillus sp. SW7) with multiple plant growth-promoting traits promotes the growth of tomato (Solanum Lycopersicum)

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