Potential of anaerobic bacteria in bioremediation of metal-contaminated marine and estuarine environment

Binish, M.B.; Binu, Prakash; Gopikrishna, V.G.; Mohan, Mahesh

doi:10.1016/b978-0-323-85455-9.00015-1

Cited by 5 publications

(8 citation statements)

References 133 publications

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“…Terefore, to achieve stability for prolong periods the yielded particle needs to be lyophilized [134]. Te minimum inhibitory concentration (MIC) of Ag-NPs for inhibiting bacterial was measured based on transmittance spectra obtained from UV-Vis spectroscopy [84,85,135]. Te turbidity of the broth due to bacterial growth showed a reciprocal relationship with the transmittance value, while transmittance ranges from 100 to 96% utilized as an indicator for bacterial growth inhibition as mentioned elsewhere [136].…”

Section: Discussionmentioning

confidence: 99%

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Facile Synthesis and Application of Ag-NPs for Controlling Antibiotic-ResistantPseudomonas spp. and Bacillus spp. in a Poultry Farm Environment

Rahman

Rasid

Ali

et al. 2023

Journal of Nanotechnology

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This study synthesized silver nanoparticles (Ag-NPs) using silver nitrate (AgNO3) as the ion source and sodium tripolyphosphate (STPP) as reducing as well as capping agents. The synthesized Ag-NPs were confirmed initially using Ag-NPs specific λmax at 410 nm with UV-Vis spectrophotometry and homogenously distributed, 100–300 nm size, and round-shaped particles were realized through atomic force microscopy (AFM) and transmission electron microscopy (TEM) image analysis. The various reaction condition-based studies revealed 0.01 M AgNO3 yields maximum particle after 4 h reduction with 1% STPP. Bacillus spp. (n = 23/90) and Pseudomonas spp. (n = 26/90) were isolated from three different poultry farms for evaluating the antibacterial activity of Ag-NPs. Among the PCR confirmed isolates, 52% (12/23) Bacillus spp. were resistant to ten antibiotics and 65% (17/26) Pseudomonas spp. were resistant to eleven antibiotics. The representative resistant isolates were subjected to antibacterial evaluation of synthesized Ag-NPs following the well diffusion method, revealing the maximum sensitive zone of inhibition 19 ± 0.2 mm against Bacillus spp. and 17 ± 0.38 mm against Pseudomonas spp. The minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) of Ag-NPs were 2.1 μg/ml and 8.4 μg/ml, respectively, for broad-spectrum application. Finally, the biocompatibility was determined by observing the viability of Ag-NP-treated BHK-21 cell through trypan blue-based exclusion assay revealing nonsignificant decreased of cell viability ≤2MIC doses. Thus, the synthesized Ag-NPs were proven as biocompatible and sensitive to both Gram-positive and Gram-negative bacteria of the poultry farm environmental samples.

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Section: Discussionmentioning

confidence: 99%

“…Data obtained from inhibitory zones were used for categorizing levels of sensitivity/resistance according to CLSI 2019. ) compared with control turbidity [85]. Te maximum percentages (100−95%) of transparency with an inhibited bacterial growth were considered as MIC [86].…”

Section: Determination Of Antibacterial Activity and Stability Ofmentioning

confidence: 99%

Facile Synthesis and Application of Ag-NPs for Controlling Antibiotic-ResistantPseudomonas spp. and Bacillus spp. in a Poultry Farm Environment

Rahman

Rasid

Ali

et al. 2023

Journal of Nanotechnology

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“…Some metals originating from different industrial processes are water-soluble, thus being easily transported to soil and groundwater systems, causing their magnification [ 3 ]. High concentrations found in agricultural soils due to application of different chemicals which are used for plant protection lead to deleterious effects on plants and ecosystem biodiversity, and, in cases of long-term exposure, human health is also affected [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Removal of heavy metals with traditional physicochemical remediation techniques is time-consuming and expensive [ 4 ]. In recent years, bioremediation techniques, which include the use of microorganisms or plants for cleaning up the environment, have been exploited, as they are cost-effective and environmentally friendly [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF

et al. 2023

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Soil pollution with heavy metals is a serious threat to the environment. However, soils polluted with heavy metals are considered good sources of native metal-resistant Trichoderma strains. Trichoderma spp. are free-living fungi commonly isolated from different ecosystems, establishing endophytic associations with plants. They have important ecological and biotechnological roles due to their production of a wide range of secondary metabolites, thus regulating plant growth and development or inducing resistance to plant pathogens. In this work we used indigenous Trichoderma strains that were previously isolated from different soil types to determine their tolerance to increased copper and nickel concentrations as well as mechanisms of metal removal. The concentrations of bioavailable metal concentrations were determined after extraction with diethylene-triamine pentaacetate (DTPA)-extractable metals (Cd, Cr, Co, Cu, Pb, Mn, Ni, and Zn) from the soil samples by inductively coupled plasma-optical emission spectrometry (ICP-OES). Two indigenous T. harzianum strains were selected for copper tolerance, and three indigenous T. longibrachiatum strains were selected for nickel tolerance tests. Strains were isolated from the soils with the highest and among the lowest DTPA-extractable metal concentrations to determine whether the adaptation to different concentrations of metals affects the mechanisms of remediation. Mechanisms of metal removal were determined using Fourier-transform infrared spectroscopy (FTIR) and X-ray fluorescence spectroscopy (XRF), non-destructive methods characterized by high measurement speed with little or no need for sample preparation and very low costs. Increased DTPA-extractable metal content for nickel and copper was detected in the soil samples above the target value (TV), and for nickel above the soil remediation intervention values (SRIVs), for total metal concentrations which were previously determined. The SRIV is a threshold of metal concentrations indicating a serious soil contamination, thus confirming the need for soil remediation. The use of FTIR and XRF methods revealed that the presence of both biosorption and accumulation of metals in the Trichoderma cells, providing good bioremediation potential for Ni and Cu.

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“…The strain was able to utilise ammonia, nitrate, and nitrite as a sole nitrogen source [ 14 ]. P. bifermentans has been shown to have heavy metal bioremediation potential; a P. bifermentans strain isolated from an estuary was able to remove 81% (0.6

g g

) of dissolved mercury(II) from solution [ 15 ]. The use of bioremediation has shown promising results, and previous work completed using a consortium obtained from a battery recycling plant in Gauteng, South Africa, has shown removals of circa 49% and 93% of 80 mg L

initial Pb(II) concentration after 3 h and 12 d, respectively.…”

Section: Introductionmentioning

confidence: 99%

Microbial Precipitation of Pb(II) with Wild Strains of Paraclostridium bifermentans and Klebsiella pneumoniae Isolated from an Industrially Obtained Microbial Consortium

Neveling

Ncube

Ngxongo

et al. 2022

IJMS

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The study focused on determining the microbial precipitation abilities of bacterial strains that were isolated from an industrially obtained Pb(II)-resistant microbial consortium. Previous research has demonstrated the effectiveness of the consortium on the bioprecipitation and adsorption of Pb(II) from solution. The bioremediation of Pb(II) using microbial precipitation provides an alternative option for Pb(II) removal from wastewater. Both strains, Klebsiella pneumoniae and Paraclostridium bifermentans, were successfully isolated from the consortium obtained from a battery recycling plant in South Africa. The experiments were conducted over both 30 h and 5 d, providing insight into the short- and long-term precipitation abilities of the bacteria. Various initial concentrations of Pb(II) were investigated, and it was found that P. bifermentans was able to remove 83.8% of Pb(II) from solution with an initial Pb(II) concentration of 80 mg L−1, while K. pneumoniae was able to remove 100% of Pb(II) with the same initial Pb(II) concentration after approximately 5 d. With the same initial Pb(II) concentration, P. bifermentans was able to remove 86.1% of Pb(II) from solution, and K. pneumoniae was able to remove 91.1% of Pb(II) from solution after 30 h. The identities of the precipitates obtained for each strain vary, with PbS and Pb0 being the main species precipitated by P. bifermentans and PbO with either PbCl or Pb3(PO4)2 precipitated by K. pneumoniae. Various factors were investigated in each experiment, such as metabolic activity, nitrate concentration, residual Pb(II) concentration, extracellular and intracellular Pb(II) concentration and the precipitate identity. These factors provide a greater understanding of the mechanisms utilised by the bacteria in the bioprecipitation and adsorption of Pb(II). These results can be used as a step towards applying the process on an industrial scale.

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Potential of anaerobic bacteria in bioremediation of metal-contaminated marine and estuarine environment

Cited by 5 publications

References 133 publications

Facile Synthesis and Application of Ag-NPs for Controlling Antibiotic-ResistantPseudomonas spp. and Bacillus spp. in a Poultry Farm Environment

Facile Synthesis and Application of Ag-NPs for Controlling Antibiotic-ResistantPseudomonas spp. and Bacillus spp. in a Poultry Farm Environment

Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF

Microbial Precipitation of Pb(II) with Wild Strains of Paraclostridium bifermentans and Klebsiella pneumoniae Isolated from an Industrially Obtained Microbial Consortium

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