A feeding trial was conducted to study the e¡ect of six iso-energetic diets containing 25, 30, 35, 40, 45 and 50% crude protein (CP) on growth, survival and feed conversion ratio (FCR) as well as the protein requirement of an endangered cyprinid,Tor putitora. Triplicate groups of ¢ngerlings with initial total length of 10.0^11.0 cm and weight of 12.0^12.5 g were reared in earthen ponds and fed diets at 5% of body weight for 120 days. Performance was evaluated on the basis of total length gain, body weight gain, survival rate, feed e⁄ciency, FCR, protein e⁄ciency ratio, speci¢c growth rate, energy retention, gross and net yield in kg ha À1 . Whole-body carcass composition of ¢sh was analysed at the start and the end of the experiment. Growth and FCR were in£uenced signi¢cantly (Po0.05) by dietary CP contents; higher growth and lower FCRs were obtained with increasing dietary protein. Dietary protein also in£uenced the whole-body carcass composition of the ¢sh. Higher protein and ash, and lower moisture and lipid in the whole body were observed with increasing dietary protein. Broken-line regression analysis indicated that the optimum dietary protein level for maximal growth of mahseer lies between 45% and 50% (45.3%). Overall feed utilization and growth performance of the ¢sh is comparable to that of other aquaculture species and the ¢sh o¡ers high potential for commercial aquaculture. Ã Carboxymethyl cellulose (high viscosity).wMinerals (mg g À1 ): zinc 510.0; iron 516.6; copper 51.0; manganese 5 6.6; iodine 5 0.33; cobalt 5 0.033; selenium 5 0.033; phosphorus 5 60.0; ethoxyquine (antioxidant) 5 40.0. zVitamins (in each g of premix): A 5 5000 IU; B 1 55 mg; B 2 58 mg; B 6 5 4 mg; B 12 50.01mg; C 5 25 mg; E 5 50 mg; D 3 5500 IU; K 3 54 mg; niacin 5 40 mg; D-calcium^pentothenate 516.6 mg; folic acid 51.33 mg; D-Biotin 5 0.17 mg; inositol 5 50 mg. ‰NFE, nitrogen-free extract, calculated as 100 À % (CP1crude lipid1ash1crude ¢ber). CP, crude protein. Aquaculture Research, 2004, 35, 1270^1276 Dietary protein requirement for pond-reared mahseer M S Islam & M Tanaka r