Potential native Trichoderma strains against Fusarium verticillioides causing post flowering stalk rot in winter maize

Jambhulkar, Prashant P.; Raja, M.; Singh, Bhim; Katoch, Sonali; Kumar, Shailendra; Sharma, Pratibha

doi:10.1016/j.cropro.2021.105838

Cited by 17 publications

(11 citation statements)

References 29 publications

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“…This showed that F. verticillioides is the major pathogen causing PFSR. Our results support the previous finding that F. verticillioides is the dominant Fusarium spp causing PFSR in India (Swamy et al, 2019;Jambhulkar et al, 2022). Among the virulent strain F. verticillioides (Davanagere, Raichur, F13, FUG9, FUR15, F21, and F59), except the FUG9 and FUR 15, remaining strains had purple to dark purple pigmentation.…”

Section: Discussionsupporting

confidence: 91%

“…High temperature and relative humidity of southern Rajasthan than in other Rabi maize-growing states make it a hot spot for PFSR in maize. Thus, such climatic conditions are responsible for causing significant economic loss by PFSR in the eastern side of the Central Plateau and hills, the northern part of the Western plateau and hills, and southern plains and hills as compared to other agroclimatic zones (Czembor et al, 2015;Jambhulkar et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…PFSR-infected maize plants exhibit symptoms of drooping, wilting, and drying of leaves, empty cob development, and an increase in the angle between stalks and cobs in the field. Fusarium verticillioides has been reported as a major fungal species of PFSR (Venturini et al, 2013;Yu et al, 2017;Jambhulkar et al, 2022). The isolates of Fusarium spp.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, being a soilborne pathogen, management of the disease through conventional measures is difficult such as fungicide application which is cost-prohibitive and destructive to ecology. A substantial amount of work has been done to investigate the use of biological control to address the issue of PFSR (Chandra Nayaka et al, 2010;Wu et al, 2015;Lu et al, 2020;Jambhulkar et al, 2022), but breeding resistant germplasm is the ultimate solution to reduce crop loss due to PFSR. Therefore, stable resistant inbred lines against virulent isolates of Fusarium spp.…”

Section: Introductionmentioning

confidence: 99%

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Morphological characterization, pathogenicity screening, and molecular identification of Fusarium spp. isolates causing post-flowering stalk rot in maize

et al. 2023

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Post flowering stalk rot (PFSR) of maize caused by the Fusarium species complex is a serious threat to maize production worldwide. The identification of Fusarium species causing PFSR based on morphology traditionally relies on a small set of phenomic characteristics with only minor morphological variations among distinct Fusarium species. Seventy-one isolates were collected from 40 sites in five agro-climatic zones of India to assess the diversity of Fusarium spp. associated with maize crops showing symptoms of PFSR in the field. To investigate the pathogenicity of Fusarium spp. causing PFSR sixty isolates were toothpick inoculated between the first and second node at 55 days after sowing during the tassel formation stage of the crop in Kharif (Rainy season), and Rabi (Winter season) season field trials. Ten most virulent Fusarium isolates, based on the highest observed disease index, were identified by homology and phylogenetic analyses of partial sequences of the translation elongation factor 1 α (Tef-1α). Based on morphological traits such as mycelial growth patterns and mycelial pigmentation, Fusarium isolates were divided into nine clusters. The isolates were judged to be virulent based on their ability to decrease seedling vigour in in-vivo situations and high disease severity in field experiments. Pathogenicity test during the Kharif season showed 12 isolates with virulent disease symptoms with a mean severity ranging between 50 to 67 percent disease index (PDI) whereas in Rabi season, only five isolates were considered virulent, and the mean severity ranged between 52 to 67 PDI. Based on pathological characterization and molecular identification, 10 strains of Fusarium species namely, Fusarium acutatum (2/10), Fusarium verticillioides (Syn. Gibberella fujikuroi var. moniliformis) (7/10), Fusarium andiyazi (2/10) recorded the highest diseases index. All these species are part of the Fusarium fujikuroi species complex (FFSC). The distribution of virulent isolates is specific to a geographical location with a hot humid climate. Increased knowledge regarding the variability of Fusarium spp. responsible for PFSR of maize occurring across wide geographical locations of India will enable more informed decisions to be made to support the management of the disease, including screening for resistance in maize-inbred lines.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphological characterization, pathogenicity screening, and molecular identification of Fusarium spp. isolates causing post-flowering stalk rot in maize

et al. 2023

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“…Our study indicated 10:3 ratio among T. asperellum and T. asperelloides within the strains isolated in India indicating the dominance of T. aseprellum over T. asperelloides in the isolation region. Both of these species have been used in biocontrol applications [42][43][44] . Longibrachiatum section consisting of two strains, T. longibrachiatum BTlg15 and T. ghanense BTgh8 known producers of cellulose hydrolyzing enzymes (particularly T. reesei 45,46 ), as cause of opportunistic infections of man and animals 47,48 .…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and antagonistic properties of Trichoderma strains from the crop rhizospheres in southern Rajasthan, India

Jambhulkar,

Singh,

Raja

et al. 2024

Sci Rep

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There are fewer studies on Trichoderma diversity in agricultural fields. The rhizosphere of 16 crops was analyzed for Trichoderma species in 7 districts of Rajasthan state of India. Based on DNA sequence of translation elongation factor 1α (tef-1α), and morphological characteristics, 60 isolates were identified as 11 species: Trichoderma brevicompactum, species in Harzianum clade identified as T. afroharzianum, T. inhamatum, T. lentiforme, T. camerunense, T. asperellum, T. asperelloides, T. erinaceum, T. atroviride, T. ghanense, and T. longibrachiatum. T. brevicompactum is the most commonly occurring strain followed by T. afroharzianum. No new species were described in this study. T. lentiforme, showed its first occurrence outside the South American continent. The morphological and cultural characteristics of the major species were observed, described, and illustrated in detail. The isolates were tested for their antagonistic effect against three soilborne plant pathogens fungi: Sclerotium rolfsii, Rhizoctonia solani, and Fusarium verticillioides in plate culture assays. One of the most potent strains was T. afroharzianum BThr29 having a maximum in vitro inhibition of S. rolfsii (76.6%), R. solani (84.8%), and F. verticillioides (85.7%). The potential strain T. afroharzianum BThr29 was also found to be efficient antagonists against soil borne pathogens in in vivo experiment. Such information on crop selectivity, antagonistic properties, and geographic distribution of Trichoderma species will be beneficial for developing efficient Trichoderma-based biocontrol agents.

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Biocontrol of Fusarium spp. root rot in yerba mate (Ilex paraguariensis) by native rhizospheric Trichoderma spp.

Quevedo,

Muniz,

Sarzi

et al. 2024

BioControl

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Potential native Trichoderma strains against Fusarium verticillioides causing post flowering stalk rot in winter maize

Cited by 17 publications

References 29 publications

Morphological characterization, pathogenicity screening, and molecular identification of Fusarium spp. isolates causing post-flowering stalk rot in maize

Morphological characterization, pathogenicity screening, and molecular identification of Fusarium spp. isolates causing post-flowering stalk rot in maize

Genetic diversity and antagonistic properties of Trichoderma strains from the crop rhizospheres in southern Rajasthan, India

Biocontrol of Fusarium spp. root rot in yerba mate (Ilex paraguariensis) by native rhizospheric Trichoderma spp.

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