Potential mechanisms underlying embryonic developmental toxicity caused by benzo[a]pyrene in Japanese medaka (Oryzias latipes)

Yamaguchi, Akemi; Uchida, Masaya; Ishibashi, Hiroshi; Hirano, Masashi; Ichikawa, Nobuhiro; Arizono, Koji; Koyama, Jiro; Tominaga, Nobuaki

doi:10.1016/j.chemosphere.2019.125243

Cited by 13 publications

(9 citation statements)

References 40 publications

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“…However, long-term immersion is required in a high-concentration solution, owing to which the window of exposure becomes unclear. Notably, nsPEF treatment is a potent method for incorporation of chemical compounds into medaka eggs (Tominaga et al, 2010(Tominaga et al, , 2019Yamaguchi et al, 2018Yamaguchi et al, , 2020. Using this method, chemical substances that are difficult to introduce via osmosis alone can be easily introduced inside medaka eggs with shells.…”

Section: Resultsmentioning

confidence: 99%

“…Chemical incorporation and subsequent observation were performed as described by Yamaguchi et al (2020). Fertilized medaka eggs from various breeding pairs were collected within 5 hr of fertilization and then randomly selected for treatment.…”

Section: Chemical Incorporation Into Medaka Fertilized Eggs Using Nspef and Morphology Observationsmentioning

confidence: 99%

“…We previously developed an electroporation method using a nanosecond pulsed electric field (nsPEF) to facilitate the introduction of substances into medaka eggs. We attempted to assess teratogenicity and embryonic developmental toxicity; moreover, we determined some endpoints that included not only survival, growth, and developmental abnormalities but also changes in the gene expression levels for the assessment of toxicity of 17β-estradiol, lithium, and benzo(a)pyrene using medaka and nsPEF treatment (Yamaguchi et al, 2018(Yamaguchi et al, , 2020Tominaga et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electroporation of thalidomide to medaka (<i>Oryzias latipes</i>) embryo for evaluation of developmental toxicity

Nishiyama

Uchida

Terada

et al. 2021

Fundam. Toxicol. Sci.

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Section: Resultsmentioning

confidence: 99%

Section: Chemical Incorporation Into Medaka Fertilized Eggs Using Nspef and Morphology Observationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electroporation of thalidomide to medaka (<i>Oryzias latipes</i>) embryo for evaluation of developmental toxicity

Nishiyama

Uchida

Terada

et al. 2021

Fundam. Toxicol. Sci.

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“…Numerous studies have documented that various PAHs can inhibit mechanisms associated with bone formation and enhance bone resorption in fish. In Japanese medaka (Oryzias latipes), it has been proposed that PAHs, such as benzo(a)pyrene, typically cause perturbed chondroblast and osteoblast differentiation due to differential expression of key genes regulating osteoblast-mediated bone formation, resulting in a defective notochord sheath repair, and rendering the vertebral column more vulnerable to physical damage (Yamaguchi et al, 2020). In rockfish (Sebastiscus marmoratus) exposed to benzo(a)pyrene during embryogenesis, He et al (2011) suggested that PAHs act by perturbing the proliferation of chondrocytes and impairing the expression of sonic hedgehog pathway genes, leading to disturbed craniofacial formation.…”

Section: Mechanisms Of Actionmentioning

confidence: 99%

Ontogeny-Specific Skeletal Deformities in Atlantic Haddock Caused by Larval Oil Exposure

et al. 2021

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Bone deformities are one of the main effects of crude oil exposure in marine fish larvae. Craniofacial and jaw deformities, if severe enough, may restrict feeding and ultimately kill the developing larvae. This study aimed to examine the impact of dispersed crude oil on bone development in Atlantic haddock (Melanogrammus aeglefinus) larvae, a fish species spawning in areas approached for oil and gas exploration in the North Atlantic Ocean. Atlantic haddock larvae were exposed to low (60 μg oil/L), high (600 μg oil/L), or pulsed (0–600, average 60 μg oil/L over time) dispersed crude oil from 0 to 18 days post hatch (dph). Endpoints included survival and growth, bone integrity, and transcriptional parameters, which were assessed during (0–18 dph) and after exposure until the fish reached 8 months of age (243 dph). The results showed that the larvae in the high treatment group had reduction in growth at 2–19, 44, 134, and 243 dph. Craniofacial abnormalities were most severe at 8 and 19 dph. These deformities were not present at 44 dph, possibly because the larvae with deformed jaws failed to feed properly and died. Higher prevalence of spinal deformities was observed in haddocks that survived for 243 dph. Three genes encoding proteins critical for osteoblast function, sp7, postn, and col10a1, were downregulated in the high treatment group larvae. We discuss possible mechanisms of action in the developing larvae after oil exposure. In conclusion, this study shows that larval exposure to oil can potentially have long-term effects on growth and bone integrity in Atlantic haddock.

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“…Which has the characteristics of lipophilicity, refractory, bioaccumulation, cytotoxicity, mutagenicity and carcinogenicity. 13,14 Benzo[α]pyrene (BaP) is the most common PAHs, and BaP exposure is closely related to the incidence of lung cancer. 15 As we all know, BaP is a classic exogenous ligand of Aryl hydrocarbon receptor (AhR).…”

Section: Introductionmentioning

confidence: 99%

Study on Molecular Mechanism of Benzo (ɑ) pyrene on CMA by HSP90ɑ and HIF-1ɑ

Zhang

Liu

Chen

et al. 2021

Preprint

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OBJECTIVE: The effects of benzo (α) pyrene (BaP) on molecular chaperone autophagy (CMA) through heat shock protein 90 (HSP90) and hypoxia inducible factor-1 (HIF-1) were studied by RNA interference and subcutaneous tumor formation technique in nude mice. METHODS: 40 nude mice inoculated with the shHSP90ɑ A549 cell line under the armpits of the forelimbs were divided into 4 groups, 1.80 mg/kg/d BaP-corn oil solution was intragastrically administered for 60 days (except the Control group), and the growth of nude mice and transplanted tumors was recorded curve. The size and morphological changes of tumors were observed by small animal imaging technique and HE staining method. qPCR, Western blot and Immunohistochemistry were used to detect the expression of HSP90ɑ, HSC70 and Lamp-2A. A549 cells were treated with 0.1μmol/L, 1μmol/L and 10μmol/L BaP for 24h, EPO, HIF-1ɑ concentration and HIF-1ɑ protein expression were detected by Elisa and Western blot; A549 cells were treated with 10μmol/L BaP and added HIF-1ɑ inhibitor 24h, qPCR, Western blot and Immunofluorescence method were used to detect the expression of HSP90ɑ, HSC70 and Lamp-2A. RESULTS: BaP can reduce silence HSP90ɑ the weight of nude mice and transplanted tumors (P<0.01); BaP can reduce silence HSP90ɑ the expression of HSP90ɑ, HSC70, Lamp-2A mRNA and protein in transplanted tumor tissues (P<0.05); BaP has no significant difference in the organization structure of silence HSP90ɑ and non-silence HSP90ɑ; BaP can reduce the total number of bioluminescence photons of silence HSP90ɑ transplanted tumors (P<0.01). 10μmol/L BaP can increase the concentration of EPO and HIF-1ɑ and the expression of HIF-1ɑ protein in A549 cells (P<0.05); and after adding HIF-1ɑ inhibitors treat A549 cells, HSP90ɑ, HSC70, Lamp-2A mRNA and protein expression were decreased (P<0.05), and the fluorescence intensity of HSP90ɑ was decreased. CONCLUSIONS: BaP can promote the growth of transplanted tumor in nude mice, while the growth of transplanted tumor in nude mice is inhibited shHSP90ɑ. BaP promotes the occurrence of CMA by promoting the expression of HSP90ɑ and HIF-1ɑ, which are important regulatory genes for BaP to activate CMA.

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Potential mechanisms underlying embryonic developmental toxicity caused by benzo[a]pyrene in Japanese medaka (Oryzias latipes)

Cited by 13 publications

References 40 publications

Electroporation of thalidomide to medaka (<i>Oryzias latipes</i>) embryo for evaluation of developmental toxicity

Electroporation of thalidomide to medaka (<i>Oryzias latipes</i>) embryo for evaluation of developmental toxicity

Ontogeny-Specific Skeletal Deformities in Atlantic Haddock Caused by Larval Oil Exposure

Study on Molecular Mechanism of Benzo (ɑ) pyrene on CMA by HSP90ɑ and HIF-1ɑ

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