HistologyAgarose was fixed, dehydrated, processed, embedded in paraffin, and sectioned at 8 µm thickness. Sections were stained with Safranin-O/hematoxylin using standard protocols. Brightfield images were taken at 20X magnification on a VS120 microscope (Olympus).
Live Dead imagingConstructs were stained for 30 minutes using the live/dead dye (ThermoFisher, Calcein-AM and Ethidium homodimer-1). Then the samples were imaged using a confocal microscope with the laser powers that were suggested by ThermoFisher.