Potential mechanisms mediating the protective effects of a peptide from walnut (<i>Juglans mandshurica</i>Maxim.) against hydrogen peroxide induced neurotoxicity in PC12 cells

Liu, Chunlei; Guo, Yong; Zhao, Fanrui; Qin, Hanxiong; Lu, Huiqi; Li, Fang; Wang, Ji; Min, Weihong

doi:10.1039/c8fo02557f

Cited by 58 publications

(33 citation statements)

References 41 publications

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“…Immunohistochemistry is a semiquantitative experiment. On the basis of the principle of specific combination of antigen and antibody, the internal antigen of tissues and cells was determined by chemical reaction to make the chromogenic reagent of labeled antibody color (Liu et al., 2019). TNF‐α is a cytokine secreted by T‐lymphocytes and activated macrophages.…”

Section: Resultsmentioning

confidence: 99%

Protective effect againstd‐gal‐induced aging mice and components of polypeptides and polyphenols in defatted walnut kernel during simulated gastrointestinal digestion

Tian

Sun

et al. 2021

Journal of Food Science

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Defatted walnut kernel with pellicle (WKP) is an industrial byproduct during walnut oil extraction, which is rich in protein and polyphenols. WKP was hydrolyzed by simulated gastrointestinal digestion to obtain WKP hydrolysates (WKPHs). Results showed the protein recovery and hydrolysis degree of WKPH were 82.15 and 10.36%. The total phenol contents in WKP and WKPH were 4.90 and 40.70 mg gallic acid equivalent/g, respectively. The antiaging activity of WKPH was evaluated using a d‐gal‐induced aging mouse model. Results showed that WKPHs could recover the activities of SOD and T‐AOC and the content of MDA in tissues and serum of the aging mice. The histological morphology of liver and kidney sections and the immunohistochemistry of TNF‐α, IL‐1β, and IL‐6 in liver were observed. WKPH could effectively protect the tissue structure of the liver and kidney and reduce the inflammatory expression of liver in aging mice. The polypeptides and polyphenols in WKPH were further analyzed. Fifty polypeptides were identified and 12 of these peptides had Leu‐Arg at the C‐terminal. Forty‐two polyphenols were detected, and most phenolic compounds belonged to ellagitannins. This study provided a theoretical basis for the improved processing and high‐value utilization of walnut byproducts. Practical Application Defatted walnut kernel with pellicle was hydrolyzed by simulated gastrointestinal digestion to obtain its hydrolysates. The hydrolysates have good antiaging activity in vivo. Fifty polypeptides were identified and 12 of these peptides had Leu‐Arg at the C‐terminal. Forty‐two polyphenols were detected, and most phenolic compounds belonged to ellagitannins. This study could provide a theoretical basis for high‐value utilization of walnut byproducts.

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Section: Resultsmentioning

confidence: 99%

Protective effect againstd‐gal‐induced aging mice and components of polypeptides and polyphenols in defatted walnut kernel during simulated gastrointestinal digestion

Tian

Sun

et al. 2021

Journal of Food Science

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“…PC12 rat pheochromocytoma cells are frequently used as a model in neuronal research (36). PC12 cells were purchased from Procell Life Science & Technology Co., Ltd. and were cultured at 37°C in a 5% CO 2 incubator in RPMI 1640 medium (Gibco; Thermo Fisher Scientific, Inc.), 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 1% solution penicillin/streptomycin (Thermo Fisher Scientific, Inc.).…”

Section: Methodsmentioning

confidence: 99%

miR‑134‑5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model

Liu

Zhang

et al. 2019

Int J Mol Med

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Forkhead box P2 (Foxp2) is a transcription factor involved in vocal learning. However, the number of previous studies that have investigated the role of Foxp2 in early vascular dementia (Vd) is limited. The aim of the present study was to determine whether microRNA (miR)-134-5p/Foxp2 contributes to cognitive impairment in a chronic ischemia-induced early Vd model. miR-134-5p was found to be significantly increased in the cortex in a rat Vd model. Intracerebroventricular injection of miR-134-5p antagomir into Vd rats prevented the loss of synaptic proteins and the development of cognitive impairment phenotypes. Histopathological analysis revealed that miR-134-5p aggravated cognitive impairment in Vd rats through damage to cortical neurons and loss of synaptic proteins. Bioinformatics analysis predicted that miR-134-5p targets Foxp2 mRNA. dual luciferase analysis and western blotting supported the prediction that miR-134-5p targets Foxp2. Furthermore, the silencing of Foxp2 significantly inhibited the effect of miR-134-5p on synaptic protein loss. chromatin immunoprecipitation-quantitative polymerase chain reaction analysis indicated that Foxp2 binds to the synapsin I (Syn1) promoter at-400/-600 bp upstream of the transcription start site. In conclusion, the miR-134-5p/Foxp2/Syn1 axis was found to contribute to cognitive impairment in a chronic ischemia-induced early Vd model, which may enable the development of new therapeutic strategies for the prevention and treatment of Vd.

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“…Antioxidant peptides (APs) isolated from food proteins have drawn great public attention because of their high-level safety and strong ROS scavenging, cytoprotective, and DNA protection capacities [12][13][14]. EVSGPGLSPN from walnut could significantly (p < 0.05) reduce ROS generation, and dose-dependently increase cell viability of H 2 O 2 -induced PC12 cells by enhancing the levels of intracellular antioxidant enzymes and blocking the nuclear factor-κB (NF-κB)/caspase pathway [15]. IYVVDLR and IYVFVR from alcalase-hydrolysate of soybean could strongly scavenge radicals, upregulate total reduced glutathione (GSH) synthesis, enhance activities of intracellular antioxidant enzyme (catalase (CAT) and glutathione reductase (GR), and suppress ROS-mediated inflammatory responses via inhibiting interleukin-8 secretion in Caco-2 cells [16].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2

Wang

Zhao

et al. 2020

Marine Drugs

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In the work, defatted muscle proteins of monkfish (Lophius litulon) were separately hydrolyzed by pepsin, trypsin, and in vitro gastrointestinal (GI) digestion methods, and antioxidant peptides were isolated from proteins hydrolysate of monkfish muscle using ultrafiltration and chromatography processes. The antioxidant activities of isolated peptides were evaluated using radical scavenging and lipid peroxidation assays and H2O2-induced model of HepG2 cells. In which, the cell viability, reactive oxygen species (ROS) content, and antioxidant enzymes and malondialdehyde (MDA) levels were measured for evaluating the protective extent on HepG2 cells damaged by H2O2. The results indicated that the hydrolysate (MPTH) prepared using in vitro GI digestion method showed the highest degree of hydrolysis (27.24 ± 1.57%) and scavenging activity on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (44.54 ± 3.12%) and hydroxyl radical (41.32 ± 2.73%) at the concentration of 5 mg protein/mL among the three hydrolysates. Subsequently, thirteen antioxidant peptides (MMP-1 to MMP-13) were isolated from MPTH. According to their DPPH radical and hydroxyl radical scavenging activity, three peptides with the highest antioxidant activity were selected and identified as EDIVCW (MMP-4), MEPVW (MMP-7), and YWDAW (MMP-12) with molecular weights of 763.82, 660.75, and 739.75 Da, respectively. EDIVCW, MEPVW, and YWDAW showed high scavenging activities on DPPH radical (EC50 0.39, 0.62, and 0.51 mg/mL, respectively), hydroxyl radical (EC50 0.61, 0.38, and 0.32 mg/mL, respectively), and superoxide anion radical (EC50 0.76, 0.94, 0.48 mg/mL, respectively). EDIVCW and YWDAW showed equivalent inhibiting ability on lipid peroxidation with glutathione in the linoleic acid model system. Moreover, EDIVCW, MEPVW, and YWDAW had no cytotoxicity to HepG2 cells at the concentration of 100.0 µM and could concentration-dependently protect HepG2 cells from H2O2-induced oxidative damage through decreasing the levels of reactive oxygen species (ROS) and MDA and activating intracellular antioxidant enzymes of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). These present results indicated that the protein hydrolysate and isolated antioxidant peptides from monkfish muscle, especially YWDAW could serve as powerful antioxidants applied in the treatment of some liver diseases and healthcare products associated with oxidative stress.

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Potential mechanisms mediating the protective effects of a peptide from walnut (Juglans mandshuricaMaxim.) against hydrogen peroxide induced neurotoxicity in PC12 cells

Abstract: A novel neuroprotective peptide EVSGPGLSPN, which was identified from walnut protein hydrolysates, protected PC12 cells by blocking the NF-κB/caspase pathways.

Cited by 58 publications

References 41 publications

Protective effect againstd‐gal‐induced aging mice and components of polypeptides and polyphenols in defatted walnut kernel during simulated gastrointestinal digestion

Protective effect againstd‐gal‐induced aging mice and components of polypeptides and polyphenols in defatted walnut kernel during simulated gastrointestinal digestion

miR‑134‑5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model

Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2

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