Potential mechanism of GABA secretion in response to the activation of GluK1-containing kainate receptors

Maiorov, S. A.; Зинченко, В. П.; Gaidin, Sergei G.; Kosenkov, Artem M.

doi:10.1016/j.neures.2021.03.009

Cited by 10 publications

(21 citation statements)

References 34 publications

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“…The detailed protocol of rat hippocampal neuroglial cell culture preparation was described previously [ 45 , 52 ]. Briefly, P0-2 Sprague-Dawley pups were euthanized and decapitated.…”

Section: Methodsmentioning

confidence: 99%

“…Immunostaining was performed as described previously [ 45 , 52 , 53 ]. Briefly, after [Ca 2+ ] i measurements, the cultures were washed three times with PBS and fixed with freshly prepared 4% paraformaldehyde solution for 20 min.…”

Section: Methodsmentioning

confidence: 99%

“…To probe the nuclei of cells, we used Hoechst 33,342 (5 μg/mL). The developed technique of matching the images of vital [Ca 2+ ] i measurements and postvital immunostaining was described in detail in our previous works [ 45 , 52 ].…”

Section: Methodsmentioning

confidence: 99%

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Role of L-Type Voltage-Gated Calcium Channels in Epileptiform Activity of Neurons

Laryushkin

Maiorov

Зинченко

et al. 2021

IJMS

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Epileptic discharges manifest in individual neurons as abnormal membrane potential fluctuations called paroxysmal depolarization shift (PDS). PDSs can combine into clusters that are accompanied by synchronous oscillations of the intracellular Ca2+ concentration ([Ca2+]i) in neurons. Here, we investigate the contribution of L-type voltage-gated calcium channels (VGCC) to epileptiform activity induced in cultured hippocampal neurons by GABA(A)R antagonist, bicuculline. Using KCl-induced depolarization, we determined the optimal effective doses of the blockers. Dihydropyridines (nifedipine and isradipine) at concentrations ≤ 10 μM demonstrate greater selectivity than the blockers from other groups (phenylalkylamines and benzothiazepines). However, high doses of dihydropyridines evoke an irreversible increase in [Ca2+]i in neurons and astrocytes. In turn, verapamil and diltiazem selectively block L-type VGCC in the range of 1–10 μM, whereas high doses of these drugs block other types of VGCC. We show that L-type VGCC blockade decreases the half-width and amplitude of bicuculline-induced [Ca2+]i oscillations. We also observe a decrease in the number of PDSs in a cluster and cluster duration. However, the pattern of individual PDSs and the frequency of the cluster occurrence change insignificantly. Thus, our results demonstrate that L-type VGCC contributes to maintaining the required [Ca2+]i level during oscillations, which appears to determine the number of PDSs in the cluster.

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“…The detailed protocol of rat hippocampal neuroglial cell culture preparation was described previously [ 45 , 52 ]. Briefly, P0-2 Sprague-Dawley pups were euthanized and decapitated.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Role of L-Type Voltage-Gated Calcium Channels in Epileptiform Activity of Neurons

Laryushkin

Maiorov

Зинченко

et al. 2021

IJMS

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“…Cell co-cultures of hippocampal neurons and astrocytes isolated from the brain of newborn Sprague-Dawley rats (1-3 days old) were prepared as described previously [35,36]. Newborn rats were euthanized (deep inhaled anaesthesia) and then decapitated.…”

Section: Cell Culturementioning

confidence: 99%

“…The immunocytochemical technique was used to identify GABAergic neurons and evaluate the presence of calcium-binding proteins [36,37,41]. Since [Ca 2+ ] i measurements and the visualization of the bound antibodies were carried out using different microscopes, the marker grid was plotted on the bottom side of the coverslip with the cell culture to match the images.…”

Section: Immunocytochemistrymentioning

confidence: 99%

Properties of GABAergic Neurons Containing Calcium-Permeable Kainate and AMPA-Receptors

Зинченко

Kosenkov

Gaidin

et al. 2021

Life

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Calcium-permeable kainate and AMPA receptors (CP-KARs and CP-AMPARs), as well as NMDARs, play a pivotal role in plasticity and in regulating neurotransmitter release. Here we visualized in the mature hippocampal neuroglial cultures the neurons expressing CP-AMPARs and CP-KARs. These neurons were visualized by a characteristic fast sustained [Ca2+]i increase in response to the agonist of these receptors, domoic acid (DoA), and a selective agonist of GluK1-containing KARs, ATPA. Neurons from both subpopulations are GABAergic. The subpopulation of neurons expressing CP-AMPARs includes a larger percentage of calbindin-positive neurons (39.4 ± 6.0%) than the subpopulation of neurons expressing CP-KARs (14.2 ± 7.5% of CB+ neurons). In addition, we have shown for the first time that NH4Cl-induced depolarization faster induces an [Ca2+]i elevation in GABAergic neurons expressing CP-KARs and CP-AMPARs than in most glutamatergic neurons. CP-AMPARs antagonist, NASPM, increased the amplitude of the DoA-induced Ca2+ response in GABAergic neurons expressing CP-KARs, indicating that neurons expressing CP-AMPARs innervate GABAergic neurons expressing CP-KARs. We assume that CP-KARs in inhibitory neurons are involved in the mechanism of outstripping GABA release upon hyperexcitation.

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Astrocyte Regulation of Neuronal Function and Survival in Stroke Pathophysiology

Boyle,

Berghella,

Blanco-Suarez

2024

Advances in Neurobiology

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Potential mechanism of GABA secretion in response to the activation of GluK1-containing kainate receptors

Cited by 10 publications

References 34 publications

Role of L-Type Voltage-Gated Calcium Channels in Epileptiform Activity of Neurons

Role of L-Type Voltage-Gated Calcium Channels in Epileptiform Activity of Neurons

Properties of GABAergic Neurons Containing Calcium-Permeable Kainate and AMPA-Receptors

Astrocyte Regulation of Neuronal Function and Survival in Stroke Pathophysiology

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