Potential involvement of Fgf10/Fgfr2 and androgen receptor (AR) in renal fibrosis in adult male rat offspring subjected to prenatal exposure to di- n -butyl phthalate (DBP)

Sun, Wen-Lan; Zhu, Yiping; Ni, Xiushi; Jing, Da-Dao; Yao, Yuting; Ding, Wei; Liu, Zhihong; Ding, Guoxian; Jiang, Junwei

doi:10.1016/j.toxlet.2017.09.009

Cited by 29 publications

(12 citation statements)

References 20 publications

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“…We, for the first time, demonstrated that FGF10 blocked TGF-β induced fibrosis-related protein expression in primary cultured OFs and also found diminished tissue level of FGF10 in GO tissues than in non-GO tissues. Recently, Sun et al [ 15 ] reported that reduced expression of FGF10/FGFR2 was found in fibrotic retinal tissues and NRK52E cells with DBP exposure, and these findings are consistent with our results. However, there are contradictory results regarding FGF10 and fibrosis.…”

Section: Discussionsupporting

confidence: 93%

“…Gupte et al [ 14 ] reported that overexpression of FGF10 during both inflammatory and fibrotic phases attenuates bleomycin-induced pulmonary fibrosis in mice. Sun et al [ 15 ] showed that reduced expression of FGF10/FGFR2 was found in rats with di-n-butyl phthalate-induced renal fibrosis. FGF10 has been detected in the cytoplasm of cultured prostate stromal cells [ 16 ] and the nucleus of urothelial cells [ 17 ], suggesting that different subcellular localization of FGF10 can be possible underlie the specificity of FGF10 signaling in different cell type.…”

Section: Introductionmentioning

confidence: 99%

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Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves’ orbitopathy

et al. 2021

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Purpose The role of fibroblast growth factor (FGF) in orbital fibroblasts (OFs) is rarely known. In this study, we investigated the effect of FGF10 on fibrosis and the inflammation mechanism of Graves′ orbitopathy (GO). Methods Orbital tissue from GO (n = 15) and non-GO (n = 15) was obtained for this study. The mRNA and protein expression levels of FGF10 and FGF receptor 2b (FGFR2b) in orbital tissue were determined by real-time polymerase chain reaction, western blot analysis, and confocal microscopy. The effects of FGF10 on transforming growth factor (TGF)-β1 induced fibrotic proteins and interleukin (IL)-1β- or tumor necrosis factor (TNF)-α- induced inflammatory proteins were investigated using recombinant human (rh) FGF10 and small interfering (si) RNA transfection against FGF10. Results FGF10 and FGFR2b mRNA expression levels were significantly lower in GO orbital tissues than in non-GO orbital tissues (p = 0.009 and 0.005, respectively). Immunostaining of FGF10 in orbital adipose tissues showed differences in FGF10 expression between GO and control samples. Immunostaining of FGF10 was very weak in the orbital tissues of GO patients. TGF-β1-induced fibronectin, collagen Iα, α-smooth muscle actin protein expression in GO OFs was attenuated by rhFGF10 treatment and increased by knockdown of FGF10 via siFGF10 transfection. Similarly, IL-1β- or TNF-α-induced IL-6, IL-8, and cyclooxygenase-2 protein production in GO OFs was either blocked by rhFGF10 treatment or further upregulated by inhibition of FGF10 via siFGF10 transfection. Conclusions Our data demonstrate that FGF10 has beneficial effects on the inflammatory and fibrotic mechanisms of GO in primary cultured OFs, providing new insights into GO pathology and the discovery of FGF10 as a promising novel therapeutic application for the treatment of GO.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves’ orbitopathy

et al. 2021

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“…Recent studies have shown that FGF10 reduces bleomycin-induced lung fibrosis, possibly through its AT2-protective effects (25). Furthermore, FGF10/FGFR2 and androgen receptor were reduced in renal fibrosis in adult male rat offspring subjected to prenatal exposure to di-n-butyl phthalate, but the concrete mechanism remains unclear (26). However, little is known about the role of FGF10 in the pathogenesis of peritoneal fibrosis in PD.…”

Section: Peritoneal Fibrosis Is a Common Complication Of Long-term Pementioning

confidence: 99%

“…FGF10 belongs to the FGF family. The overexpression of FGF10 can significantly attenuate both the inflammatory and fibrotic phases, resulting in lung fibrosis (26). Moreover, it has been reported that TGF-␤1 inhibits FGF10 expression in lung fibroblasts (29); however, little is known about the role of FGF10 in peritoneal fibrosis.…”

Section: Fgf10 Was Suppressed In Both In Vitro and In Vivo Peritonealmentioning

confidence: 99%

MicroRNA-145 promotes the epithelial-mesenchymal transition in peritoneal dialysis-associated fibrosis by suppressing fibroblast growth factor 10

Huang

et al. 2019

Journal of Biological Chemistry

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“…PAEs similar to artificial hormones will harm men's reproductive ability and promote precocious puberty in women . Many studies have reported that some PAEs can cause adverse effects on human organs, such as the liver, kidney, and testicles . Therefore, it is necessary to establish an effective method for the determination of trace PAEs in water.…”

Section: Introductionmentioning

confidence: 99%

Polymer phase transition characteristics coupled with GC‐MS for the determination of phthalate esters

Chen

Xin

et al. 2019

J of Separation Science

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Phthalate esters are easily released from plastics materials and migrate into the soil and water environment, causing serious pollution and posing a great threat to the health of human beings. A novel temperature‐sensitive extractant combined with liquid–liquid microextraction was developed to preconcentrate three phthalates in the water environment. To optimize the extraction efficiency for the three phthalate esters, various parameters, including polymer molecular weight, salt type, salt addition, adsorption time, desorption solvent, desorption volume, and desorption time have been studied. Under optimal conditions, limits of detection and limits of quantification were in the range of 0.007–0.120 and 0.021–0.350 µg/L, respectively. Linearities varied in the range of 5–1000 µg/L, with the correlation coefficients of 0.9867–0.9997. The preconcentration factors were in the range of 25–75. The relative recoveries of the three phthalate esters were in the range of 82.2–105.6% at the spiked levels. The relative standard deviations were in the range of 0.7–9.2% based on triplicate measurements. The results indicate that the temperature‐sensitive material is a good extractant for phthalate esters in water samples.

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Potential involvement of Fgf10/Fgfr2 and androgen receptor (AR) in renal fibrosis in adult male rat offspring subjected to prenatal exposure to di- n -butyl phthalate (DBP)

Cited by 29 publications

References 20 publications

Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves’ orbitopathy

Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves’ orbitopathy

MicroRNA-145 promotes the epithelial-mesenchymal transition in peritoneal dialysis-associated fibrosis by suppressing fibroblast growth factor 10

Polymer phase transition characteristics coupled with GC‐MS for the determination of phthalate esters

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