Potential Interaction of Green Tea Extract with Hydrochlorothiazide on Diuretic Activity in Rats

Abstract

Treatment of ischemic hypertensive patients with hydrochlorothiazide can precipitate cardiac arrhythmias. The present study was undertaken to evaluate the diuretic potential of green tea alone and its effects on hydrochlorothiazide in interactive groups. Rats were treated with high (500 mg/kg, p.o.) and low (100 mg/kg, p.o.) dose of green tea extract in alone and interactive groups for 30 days. Standard, high, and low dose interactive groups received hydrochlorothiazide (10 mg/kg, p.o.) on the day of experiment. Effect of different treatments was that assessed by evaluating diuretic action, diuretic activity, percentage of saline load excreted, and sodium and potassium levels in urine. Green tea in both high and low doses showed significant diuretic potential and when it is combined with hydrochlorothiazide resulted in significant improvement in the activity compared to hydrochlorothiazide alone treated group. It can be concluded that green tea extract when combined with hydrochlorothiazide showed significant increase in diuretic activity. Most important observation of the present study is even though the combination increases the diuretic potential, it is responsible for decrease in urinary potassium loss.

Green Tea (Camellia sinensis): A Review of Its Phytochemistry, Pharmacology, and Toxicology

“…Chakraborty et al were the first to study the effects of green tea alone and in combination with hydrochlorothiazide (HCTZ) on diuretic activity. The results showed that green tea extract at doses of 100 and 500 mg/kg showed significant diuretic activity; furthermore, green tea extract also significantly reduced potassium loss in the combination group compared with the HCTZ-treated group alone [115]. Susilowati et al (2019) also reported that green tea extract at a dose of 70 mg/kg had diuretic activity equivalent to furosemide, which may be related to the increase of glomerular filtration rate by increasing blood flow and cardiac output to the kidneys [116].…”

“…In vivo [112] Diuretic activity Green tea extract 100~500 mg/mL In vivo [115] Bone-protective effect green tea polyphenols 0.1% or 0.5% concentration In vivo [117] Anti-protozoal Leishmania effect EGCG and ECG IC 50 = 27.7, 75 µM, respectively In vitro [118] and cardiac output to the kidneys [116].…”

Comparative effectiveness of the ethanol extract and infusion of green tea leaves (Camellia Sinensis L.) as a diuretic in male swiss mice

“…Furthermore, other studies state that flavonoids can increase urine volume as these compounds increase the rate of glomerular velocity (Jouad et al, 2001). Chakraborty et al (2014) prove that the flavonoids contained in green tea are responsible for inhibiting the activity of the angiotensin-converting enzyme (ACE). Green tea can inhibit ACE activity through the mechanism of mixed inhibition, and ACE inhibition is one of the main reasons for diuretic activity and increased Na + concentrations in urine.…”

“…They are also particularly useful in preventing stroke and heart failure in hypertensive patients (Grossman et al, 2011). The green tea leaf extract, when combined with hydrochlorothiazide, has been proven to significantly increase diuretic activity (Chakraborty et al, 2014). Regular consumption of green tea may also provide some protection against hypertension in humans (Negishi et al, 2004).…”

“…Research and development of medicinal plants with diuretic potentials are a priority in the development and utilization of Indonesian medicinal plants owing to the extensive and frequent use of diuretic (Lingga et al, 2014). Green tea has been reported as an effective diuretic (Chakraborty et al, 2014). At the dose of 70 mg/kg BW, the aqueous extract of its leaves possesses a diuretic property equivalent to furosemide (Susilowati, 2019).…”