Potential Interaction of <i>Plasmodium falciparum</i> Hsp60 and Calpain

Abstract

After invasion of red blood cells, malaria matures within the cell by degrading hemoglobin avidly. For enormous protein breakdown in trophozoite stage, many efficient and ordered proteolysis networks have been postulated and exploited. In this study, a potential interaction of a 60-kDa Plasmodium falciparum (Pf)-heat shock protein (Hsp60) and Pf-calpain, a cysteine protease, was explored. Pf-infected RBC was isolated and the endogenous Pf-Hsp60 and Pf-calpain were determined by western blot analysis and similar antigenicity of GroEL and Pf-Hsp60 was determined with anti-Pf-Hsp60. Potential interaction of Pf-calpain and Pf-Hsp60 was determined by immunoprecipitation and immunofluorescence assay. Mizoribine, a well-known inhibitor of Hsp60, attenuated both Pf-calpain enzyme activity as well as P. falciparum growth. The presented data suggest that the Pf-Hsp60 may function on Pf-calpain in a part of networks during malaria growth.

Expression of cysteine proteinases and cystatins in parasites and use of cysteine proteinase inhibitors in parasitic diseases. Part III: Protozoa (2): Plasmodium spp.

“…Using mizoribine as an efficient HSP-60 inhibitor, in higher concentrations it inhibited PfCALP enzymatic activity and malarial growth compared to ALLN, a typical CALP inhibitor. These results suggested the significant interaction of HSP-60 with Pf-CALP enzymatic activity [72] .…”

Development of a Rapid Fluorescent Immunochromatographic Test to Detect Respiratory Syncytial Virus

“…IFA was performed as described previously [ 34 , 35 ]. Briefly, HEP-2 cells infected with RSV at an m.o.i.…”

Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles

“…IFA was performed as described previously 48 . The first day, MDCK cells were coating on glass coverslips positioned in wells of a 6well plate and incubated at 37 °C overnight in an atmosphere of 5% CO 2 .…”