Potential Infectious Hazards of Common Bacteriological Techniques

Anderson, Raymond E.; Stein, Leon; Moss, Marcus L.; Gross, Noel H.

doi:10.1128/jb.64.4.473-481.1952

Cited by 28 publications

(8 citation statements)

References 4 publications

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“…Removal of the cap immediately after operation of the blendor allowed the escape of an aerosol containing an average of 1,500.1 viable particles/ ft3 of air sampled. Similar results were reported by Anderson et al (2). More than 98%o of the particles liberated were smaller than 5 ,u.…”

Section: Resultssupporting

confidence: 91%

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Particle Size Distribution of Serratia marcescens Aerosols Created During Common Laboratory Procedures and Simulated Laboratory Accidents

Kenny

Sabel

1968

Applied Microbiology

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Andersen air samplers were used to determine the particle size distribution of Serratia marcescens aerosols created during several common laboratory procedures and simulated laboratory accidents. Over 1,600 viable particles per cubic foot of air sampled were aerosolized during blending operations. More than 98% of these particles were less than 5 ,u in size. In contrast, 80% of the viable particles aerosolized by handling lyophilized cultures were larger than 5 ,u. Harvesting infected eggs, sonic treatment, centrifugation, mixing cultures, and dropping infectious material produced aerosols composed primarily of particles in the 1.0to 7.5-,u size range. Considerable information has been compiled in recent years concerning the aerosol spread of microorganisms during routine bacteriological and virological procedures. Operations involving withdrawal of material from vaccine bottles (2, 6, 12), inoculation of mice and the inoculation and harvesting of eggs (6, 12), handling of lyophilized cultures (14, 15, 18), pipetting (2, 10, 16), use of the inoculating loop (2, 11), blending (2,

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Section: Resultssupporting

confidence: 91%

“…, centrifuging (24), and opening of screwcapped bottles (2,21) have been of particular interest. Serratia indica and T3 coliphage were used as test organisms in most of the studies.…”

mentioning

confidence: 99%

Particle Size Distribution of Serratia marcescens Aerosols Created During Common Laboratory Procedures and Simulated Laboratory Accidents

Kenny

Sabel

1968

Applied Microbiology

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“…Aerosols may be produced as the result of an accident such as a spill, or they may be produced unintentionally by many microbiological tech niques (192). Photography (76) and air sampling (4,55,130,146,147,193) have shown that removing the lid from a Waring Blendor, removing the closure of a shaken culture, plunging a loop into a flame, and numerous other procedures may release migroorganisms into the environment. Lyo philization, animal inoculation, and egg inoculation and harvesting have also been shown to produce aerosols (143)(144)(145).…”

Section: Potential Hazardsmentioning

confidence: 99%

Laboratory-Associated Infections: Incidence, Fatalities, Causes, and Prevention

Pike¹

1979

Annu. Rev. Microbiol.

200

113

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“…However, when the cultures were shaken and bubbles formed and when the cotton plugs were wet, either by accident or vigorous shaking, organisms were released. When dry cotton plugs were removed from test tubes containing 3-week-old Coccidioides immitis cultures, air samplers arranged around the work area (7) recovered from zero to three arthroconidia (105). The older the culture, the more arthroconidia released.…”

Section: Hepatitis Bmentioning

confidence: 99%

Biological safety cabinetry.

Kruse¹,

Puckett²,

Richardson³

1991

Clinical Microbiology Reviews

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tively, not divine origin, induced disease. In 1546, Fracastorius described the agent of communicable disease as a living contagium vivum that spread by direct contact, by intermediary fomites, or through the air and postulated that these living seeds, passed from one infected animal, produced the same disease in an animal that received them. In 1676, Leeuwenhoek discovered and described bacteria, but bacteriology as a science dates from the middle of the 19th century, a result of the scientific studies of Louis Pasteur. Microbiologists are cognizant of his study disproving the theory of spontaneous generation and his work with cultures and the propagation of bacteria; however, because all of the media were fluid, these methods were not applicable for isolating a single microorganism from mixed cultures. Robert Koch's studies with nutrient gelatin and, later, solid 207

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Potential Infectious Hazards of Common Bacteriological Techniques

Cited by 28 publications

References 4 publications

Particle Size Distribution of Serratia marcescens Aerosols Created During Common Laboratory Procedures and Simulated Laboratory Accidents

Particle Size Distribution of Serratia marcescens Aerosols Created During Common Laboratory Procedures and Simulated Laboratory Accidents

Laboratory-Associated Infections: Incidence, Fatalities, Causes, and Prevention

Biological safety cabinetry.

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