Potential implications of chemokines in reproductive function: an attractive idea

Simón, Carlos; Caballero-Campo, Pedro; García-Velasco, Juan A.; Pellicer, A.

doi:10.1016/s0165-0378(98)00031-x

Cited by 55 publications

(35 citation statements)

References 121 publications

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“…Chemokines, a family of small chemotactic cytokines initially recognized for roles in leukocyte chemotaxis, have been implicated in a number of reproductive events, such as ovulation, menstruation, embryo implantation, and parturition and disease conditions, like endometriosis and cancer [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

CX3CL1 and CCL14 Regulate Extracellular Matrix and Adhesion Molecules in the Trophoblast: Potential Roles in Human Embryo Implantation1

Hannan¹,

Salamonsen²

2008

Biology of Reproduction

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Embryo implantation is a complex process involving blastocyst attachment to the endometrial epithelium and subsequent trophoblast invasion of the decidua. We have previously shown that the chemokines CX3CL1 and CCL14 are abundant in endometrial vasculature, epithelial, and decidual cells at this time, and that their receptors, CX3CR1 and CCR1, are present on invading human trophoblasts. CX3CL1 and CCL14 promote trophoblast migration. We hypothesized that these endometrial chemokines promote trophoblast migration by regulating adhesion molecules and extracellular matrix (ECM) components on the trophoblast, similar to mechanisms used in leukocyte trafficking. Trophoblast cells (AC1M-88) used previously showed a marked increase in adhesion to fibronectin following treatment with CX3CL1 and CCL14. Alterations in trophoblast adhesion and ECM following chemokine stimulation were examined using pathway-specific oligo-arrays and quantitative real-time RT-PCR. More than 30 genes were affected by CX3CL1 treatment, and 15 genes were found to be regulated by CCL14 treatment. Real-time RT-PCR quantitation revealed significant changes in the mRNA transcripts of alpha-catenin (CTNNA1), extracellular matrix protein 1 (ECM1), osteopontin (SPP1), integrin alpha 6 (ITGA6), matrix metalloproteinase 12 (MMP12), and integrin beta 5 (ITGB5) following chemokine treatment. Several of these genes have previously been implicated in implantation. Immunohistochemistry confirmed the presence of integrin alpha 6 and SPP1 protein in first-trimester human implantation sites. The temporal and spatial expression of chemokines, their receptors, adhesion, and ECM at the maternal-fetal interface emphasizes an important role in the controlled directional migration of trophoblasts through the maternal decidua. For the first time, this study demonstrates the direct effects of CX3CL1 and CCL14 on trophoblast adhesion molecules and ECM, suggesting mechanisms by which trophoblast cells migrate during early pregnancy.

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Section: Introductionmentioning

confidence: 99%

CX3CL1 and CCL14 Regulate Extracellular Matrix and Adhesion Molecules in the Trophoblast: Potential Roles in Human Embryo Implantation1

Hannan¹,

Salamonsen²

2008

Biology of Reproduction

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“…In reproductive biology, the role of these cytokines has been implicated in ovulation, menstruation, and embryo implantation, and pathological processes such as preterm delivery, and endometriosis6364. The interleukin 1 is a pro-inflammatory cytokine with multiple functions in a range of tissues65.…”

Section: Resultsmentioning

confidence: 99%

Endometrial gene expression profile of pregnant sows with extreme phenotypes for reproductive efficiency

Cordoba

Balcells

Castelló

et al. 2015

Sci Rep

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Prolificacy can directly impact porcine profitability, but large genetic variation and low heritability have been found regarding litter size among porcine breeds. To identify key differences in gene expression associated to swine reproductive efficiency, we performed a transcriptome analysis of sows’ endometrium from an Iberian x Meishan F2 population at day 30–32 of gestation, classified according to their estimated breeding value (EBV) as high (H, EBV > 0) and low (L, EBV < 0) prolificacy phenotypes. For each sample, mRNA and small RNA libraries were RNA-sequenced, identifying 141 genes and 10 miRNAs differentially expressed between H and L groups. We selected four miRNAs based on their role in reproduction, and five genes displaying the highest differences and a positive mapping into known reproductive QTLs for RT-qPCR validation on the whole extreme population. Significant differences were validated for genes: PTGS2 (p = 0.03; H/L ratio = 3.50), PTHLH (p = 0.03; H/L ratio = 3.69), MMP8 (p = 0.01; H/L ratio = 4.41) and SCNN1G (p = 0.04; H/L ratio = 3.42). Although selected miRNAs showed similar expression levels between H and L groups, significant correlation was found between the expression level of ssc-miR-133a (p < 0.01) and ssc-miR-92a (p < 0.01) and validated genes. These results provide a better understanding of the genetic architecture of prolificacy-related traits and embryo implantation failure in pigs.

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“…Not only was documented evidence found for the involvement of these cytokines in processes such as oocyte maturation, ovulation, and uterine receptivity and implantation, the literature also strongly suggests that elevated levels of these cytokines would be beneficial to these processes (11,12,(20)(21)(22)(23)(24)(25)(26)(27).…”

Section: Discussionmentioning

confidence: 99%

A differential cytokine expression profile is induced by highly purified human menopausal gonadotropin and recombinant follicle-stimulating hormone in a pre- and postovulatory mouse follicle culture model

Foster

Segers

Smart

et al. 2010

Fertility and Sterility

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Potential implications of chemokines in reproductive function: an attractive idea

Cited by 55 publications

References 121 publications

CX3CL1 and CCL14 Regulate Extracellular Matrix and Adhesion Molecules in the Trophoblast: Potential Roles in Human Embryo Implantation1

CX3CL1 and CCL14 Regulate Extracellular Matrix and Adhesion Molecules in the Trophoblast: Potential Roles in Human Embryo Implantation1

Endometrial gene expression profile of pregnant sows with extreme phenotypes for reproductive efficiency

A differential cytokine expression profile is induced by highly purified human menopausal gonadotropin and recombinant follicle-stimulating hormone in a pre- and postovulatory mouse follicle culture model

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