Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture Test

Kikuchi, Ken; Mari, Montse; Iguchi, Shigekazu; Hiramatsu, Kazumasa; Tega-Ishii, Michiru; Sansaka, Kaori; Negishi, Kenta; Shimada, Kimie; Umemura, Jun; Notake, Shigeyuki; Yanagisawa, Hideji; Takahashi, Hiroshi; Yabusaki, Reiko; Araoka, Hideki; Yoneyama, Akio

doi:10.1155/2017/4896791

Cited by 6 publications

(6 citation statements)

References 39 publications

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“…Most simulated BCB studies are done with human banked blood [ 10 , 12 , 13 – 21 ], because differences in plasma components and red blood cell enzymes between mammal species may affect bacterial growth. In addition, variations in visible signs of growth are possible due to inter-species differences in aggregation characteristics and fragility of red blood cells [ 22 – 24 ].…”

Section: Bcb Performance Evaluation Study: Set-up Considerations and ...mentioning

confidence: 99%

“…This range should be respected in in vitro BCB evaluations. Indeed, most studies using simulated blood cultures add between 5 and 125 CFU per BCB [ 8 , 12 – 16 , 19 – 21 , 29 ]. A low CFU concentration can be achieved by making serial dilutions from a 0.5 McFarland suspension containing approximately 1.5 x 10 8 CFU/mL [ 43 ], at least for Gram-negative bacteria such as E .…”

Section: Bcb Performance Evaluation Study: Set-up Considerations and ...mentioning

confidence: 99%

“…We therefore recommend using a volume of 2 mL. For adult formulations, a volume between 8 and 10 mL per BCB is often used [ 8 , 12 , 13 , 16 , 19 – 21 , 29 ] and is indeed recommended in clinical practice [ 4 ].…”

Section: Bcb Performance Evaluation Study: Set-up Considerations and ...mentioning

confidence: 99%

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Considerations in evaluating equipment-free blood culture bottles: A short protocol for use in low-resource settings

et al. 2022

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Use of equipment-free, “manual” blood cultures is still widespread in low-resource settings, as requirements for implementation of automated systems are often not met. Quality of manual blood culture bottles currently on the market, however, is usually unknown. An acceptable quality in terms of yield and speed of growth can be ensured by evaluating the bottles using simulated blood cultures. In these experiments, bottles from different systems are inoculated in parallel with blood and a known quantity of bacteria. Based on literature review and personal experiences, we propose a short and practical protocol for an efficient evaluation of manual blood culture bottles, aimed at research or reference laboratories in low-resource settings. Recommendations include: (1) practical equivalence of horse blood and human blood; (2) a diverse selection of 10 to 20 micro-organisms to be tested (both slow- and fast-growing reference organisms); (3) evaluation of both adult and pediatric bottle formulations and blood volumes; (4) a minimum sample size of 120 bottles per bottle type; (5) a formal assessment of usability. Different testing scenarios for increasing levels of reliability are provided, along with practical tools such as worksheets and surveys that can be used by laboratories wishing to evaluate manual blood culture bottles.

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Section: Bcb Performance Evaluation Study: Set-up Considerations and ...mentioning

confidence: 99%

Section: Bcb Performance Evaluation Study: Set-up Considerations and ...mentioning

confidence: 99%

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Considerations in evaluating equipment-free blood culture bottles: A short protocol for use in low-resource settings

et al. 2022

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“…No bacteria could be cultured from the debridement tissues (Table 1 ). The blood culture isolates were finally identified as Streptococcus constellatus using superoxide dismutase A sequencing and C. ramosum by 16S ribosomal DNA sequencing [ 7 ]. The minimum inhibitory concentrations (MICs) of various antibiotics were tested using Etest for C. ramosum [ 8 ] and the broth microdilution method [ 9 ] except imipenem and meropenem (Etest) for S. constellatus (Table 2 ).…”

Section: Case Presentationmentioning

confidence: 99%

Fatal Fournier’s gangrene caused by Clostridium ramosum in a patient with central diabetes insipidus and insulin-dependent diabetes mellitus: a case report

Takano

Yatabe

et al. 2018

BMC Infect Dis

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BackgroundClostridium ramosum is a generally non-pathogenic enteric anaerobe, and Fournier’s gangrene is a rare necrotizing soft tissue infection with male predisposition affecting the perineum and the genital area. We report, to our knowledge, the first case of Fournier’s gangrene caused by C. ramosum in a female patient with multiple underlying conditions.Case presentationA 44-year-old woman with a 6-year history of insulin-dependent diabetes mellitus after total pancreatectomy and an 11-year history of central diabetes insipidus developed a pain in the genital area after a month of urinary catheter use. The lower abdominal pain worsened gradually over 2 weeks, and the pain, general fatigue, and loss of appetite prompted the patient’s hospital admission. As she had severe edema in her pelvic and bilateral femoral areas, ceftriaxone was started empirically after collecting two sets of blood cultures. On hospital day 2, CT examination revealed the presence of necrotizing faciitis in the genital and pelvic areas, and the antibiotics were changed to a combination of meropenem, vancomycin, and clindamycin. Gram-positive cocci and gram-positive rods were isolated from blood cultures, which were finally identified as Streptococcus constellatus and C. ramosum using superoxide dismutase and 16S rDNA sequencing. An emergent surgery was performed on hospital day 2 to remove the affected tissue. Despite undergoing debridement and receiving combined antimicrobial chemotherapies, the patient’s clinical improvement remained limited. The patient’s condition continued to deteriorate, and she eventually died on hospital day 8. In the present case, the underlying diabetes mellitus, urinary incontinence due to central diabetes insipidus, undernutrition, and edema served as the predisposing conditions.ConclusionsC. ramosum is a potentially opportunistic pathogen among immunosuppressed persons and a rare cause of necrotizing fasciitis.

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“…Several methods and assays have been developed and tested for detection of methicillin resistance in staphylococci, in general with good results, including the StaphSR assay (BD GeneOhm, San Diego, CA; Stamper et al 2007), the BD geneOhm MRSA TM assay (BD GeneOhm, San Diego, CA; Ishikawa et al 2011), the FilmArray V R (FA; Idaho Technology, Salt Lake City, UT; Blaschke et al 2012), the Verigene Gram-Positive Blood Culture Assay (Luminex Corporation, Austin, TX), and the GeneXpert MRSA/SA BC assay (Cepheid, Sunnyvale, CA; Parta et al 2010;Spencer et al 2011;Romero-G omez et al 2013;Clerc et al 2014). Some of these assays can also detect vancomycin resistance determinants in enterococci (Kikuchi et al 2017). Among those described in the literature, the Xpert MRSA/SA BC assay has been reported to yield excellent results in a number of studies (Spencer et al 2011;Romero-G omez et al 2013;Clerc et al 2014).…”

Section: Detection Of Antibiotic-resistance Genes By Pcr-based Assaysmentioning

confidence: 99%

Recent advances in the microbiological diagnosis of bloodstream infections

Florio

Morici

Ghelardi

et al. 2017

Critical Reviews in Microbiology

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Rapid identification (ID) and antimicrobial susceptibility testing (AST) of the causative agent(s) of bloodstream infections (BSIs) are essential for the prompt administration of an effective antimicrobial therapy, which can result in clinical and financial benefits. Immediately after blood sampling, empirical antimicrobial therapy, chosen on clinical and epidemiological data, is administered. When ID and AST results are available, the clinician decides whether to continue or streamline the antimicrobial therapy, based on the results of the in vitro antimicrobial susceptibility profile of the pathogen. The aim of the present study is to review and discuss the experimental data, advantages, and drawbacks of recently developed technological advances of culture-based and molecular methods for the diagnosis of BSI (including mass spectrometry, magnetic resonance, PCR-based methods, direct inoculation methods, and peptide nucleic acid fluorescence in situ hybridization), the understanding of which could provide new perspectives to improve and fasten the diagnosis and treatment of septic patients. Although blood culture remains the gold standard to diagnose BSIs, newly developed methods can significantly shorten the turnaround time of reliable microbial ID and AST, thus substantially improving the diagnostic yield.

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Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture Test

Cited by 6 publications

References 39 publications

Considerations in evaluating equipment-free blood culture bottles: A short protocol for use in low-resource settings

Considerations in evaluating equipment-free blood culture bottles: A short protocol for use in low-resource settings

Fatal Fournier’s gangrene caused by Clostridium ramosum in a patient with central diabetes insipidus and insulin-dependent diabetes mellitus: a case report

Recent advances in the microbiological diagnosis of bloodstream infections

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